Hydroxytyrosol (Synonyms: DOPET; 3,4-Dihydroxyphenethyl alcohol; 3-Hydroxytyrosol)

Name: Hydroxytyrosol
Brand: MedChemExpress
SKU: HY-N0570
Rating: 5.0 (5 reviews)

Cat. No.: HY-N0570 Purity: 98.78%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

Hydroxytyrosol (DOPET) is a phenolic compound with anti-oxidant, anti-atherogenic, anti-thrombotic, antimicrobial, anti-inflammatory and anti-tumour effects.

For research use only. We do not sell to patients.

Hydroxytyrosol Chemical Structure

CAS No. : 10597-60-1

Size	Price	Stock	Quantity
Solution
10 mM * 1 mL in Ethanol	USD 61	In-stock	Estimated Time of Arrival: December 31
Solid or liquid
5 mg	USD 35	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 55	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 110	In-stock	Estimated Time of Arrival: December 31
100 mg	USD 176	In-stock	Estimated Time of Arrival: December 31
200 mg		Get quote
500 mg		Get quote

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Based on 5 publication(s) in Google Scholar

Other Forms of Hydroxytyrosol:

5 Publications Citing Use of MCE Hydroxytyrosol

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•Related Screening Libraries:

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Biological Activity
Purity & Documentation
References
Customer Review

Description

Hydroxytyrosol (DOPET) is a phenolic compound with anti-oxidant, anti-atherogenic, anti-thrombotic, antimicrobial, anti-inflammatory and anti-tumour effects^[1]^[2].

IC₅₀ & Target

Microbial Metabolite

Cellular Effect

Cell Line	Type	Value	Description	References
DG-75	IC₅₀	58 μg/mL Compound: 2, HT	Cytotoxicity against human DG75 cells after 48 hrs by MTT assay Cytotoxicity against human DG75 cells after 48 hrs by MTT assay	[PMID: 18823782]
HeLa	IC₅₀	70 μg/mL Compound: 2, HT	Cytotoxicity against human HeLa cells after 48 hrs by MTT assay Cytotoxicity against human HeLa cells after 48 hrs by MTT assay	[PMID: 18823782]
MDCK	CC₅₀	> 172.11 μM Compound: 4b	Cytotoxicity against MDCK cells after 72 hrs by MTT assay Cytotoxicity against MDCK cells after 72 hrs by MTT assay	[PMID: 24803363]
MDCK	IC₅₀	17.53 μM Compound: 4b	Antiviral activity against Influenza A virus (A/Vietnam/1194/2004(H5N1)) infected in MDCK cells assessed as inhibition of viral replication after 2 to 3 days by crystal violet staining-based plaque reduction assay Antiviral activity against Influenza A virus (A/Vietnam/1194/2004(H5N1)) infected in MDCK cells assessed as inhibition of viral replication after 2 to 3 days by crystal violet staining-based plaque reduction assay	[PMID: 24803363]
MRC5	IC₅₀	> 50 μM Compound: 2; HT	Cytotoxicity against human MRC5 cells assessed as inhibition of cell proliferation measured after 72 hrs by MTT assay Cytotoxicity against human MRC5 cells assessed as inhibition of cell proliferation measured after 72 hrs by MTT assay	[PMID: 27155468]
Platelet	IC₅₀	27 μM Compound: Htyr; Hydroxytyrosol	Inhibition of ADP-induced platelet aggregation in human platelet rich plasma preincubated for 10 mins Inhibition of ADP-induced platelet aggregation in human platelet rich plasma preincubated for 10 mins	[PMID: 26225717]
Platelet	IC₅₀	67 μM Compound: Htyr; Hydroxytyrosol	Inhibition of collagen-induced platelet aggregation in human platelet rich plasma preincubated for 10 mins Inhibition of collagen-induced platelet aggregation in human platelet rich plasma preincubated for 10 mins	[PMID: 26225717]
SK-BR-3	IC₅₀	201.1 μM Compound: Hydroxytyrosol	Antiproliferative activity against human SKBR3 cells after 72 hrs by MTT reduction assay Antiproliferative activity against human SKBR3 cells after 72 hrs by MTT reduction assay	[PMID: 28551177]

In Vitro

Hydroxytyrosol (DOPET) shows the inhibition of pro-inflammatory cytokines (TNF-α) and reduces the expression of cyclooxygenase-2 and inducible nitric oxide synthase (iNOS) more than 60%^[1].
? Hydroxytyrosol (DOPET) modulates the transcription factor NF-κB^[1].
? Hydroxytyrosol (DOPET) arrests the cell cycle, producing differentiation, apoptosis, or preventing DNA from oxidative stress^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Clinical Trial

Molecular Weight

154.16

Formula

C₈H₁₀O₃

CAS No.

10597-60-1

Appearance

Solid-Liquid Mixture

Color

Light yellow to yellow

SMILES

OC1=CC=C(CCO)C=C1O

Structure Classification

Initial Source

Endogenous metabolite

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Pure form	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 125 mg/mL (810.85 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : 100 mg/mL (648.68 mM; Need ultrasonic)

Ethanol : 50 mg/mL (324.34 mM; Need ultrasonic)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	6.4868 mL	32.4338 mL	64.8677 mL
5 mM	1.2974 mL	6.4868 mL	12.9735 mL
10 mM	0.6487 mL	3.2434 mL	6.4868 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (13.49 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.08 mg/mL (13.49 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.08 mg/mL (13.49 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Purity: 99.60%

Select Batch:

Data Sheet (272 KB) SDS (393 KB)

COA (245 KB) HNMR (202 KB) LCMS (99 KB)

Handling Instructions (2659 KB)

References

[1]. Vilaplana-Pérez C, et al. Hydroxytyrosol and potential uses in cardiovascular diseases, cancer, and AIDS. Front Nutr. 2014 Oct 27;1:18. [Content Brief]

[2]. Martínez L, et al. Hydroxytyrosol: Health Benefits and Use as Functional Ingredient in Meat. Medicines (Basel). 2018 Jan 23;5(1). [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

Ethanol / H₂O / DMSO	1 mM	6.4868 mL	32.4338 mL	64.8677 mL	162.1692 mL
	5 mM	1.2974 mL	6.4868 mL	12.9735 mL	32.4338 mL
	10 mM	0.6487 mL	3.2434 mL	6.4868 mL	16.2169 mL
	15 mM	0.4325 mL	2.1623 mL	4.3245 mL	10.8113 mL
	20 mM	0.3243 mL	1.6217 mL	3.2434 mL	8.1085 mL
	25 mM	0.2595 mL	1.2974 mL	2.5947 mL	6.4868 mL
	30 mM	0.2162 mL	1.0811 mL	2.1623 mL	5.4056 mL
	40 mM	0.1622 mL	0.8108 mL	1.6217 mL	4.0542 mL
	50 mM	0.1297 mL	0.6487 mL	1.2974 mL	3.2434 mL
	60 mM	0.1081 mL	0.5406 mL	1.0811 mL	2.7028 mL
	80 mM	0.0811 mL	0.4054 mL	0.8108 mL	2.0271 mL
	100 mM	0.0649 mL	0.3243 mL	0.6487 mL	1.6217 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Hydroxytyrosol Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Hydroxytyrosol10597-60-1DOPET 3,4-Dihydroxyphenethyl alcohol 3-HydroxytyrosolEndogenous MetaboliteBacterialFungalInhibitorinhibitorinhibit

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Hydroxytyrosol (Synonyms: DOPET; 3,4-Dihydroxyphenethyl alcohol; 3-Hydroxytyrosol)

Customer Review

Other Forms of Hydroxytyrosol:

Hydroxytyrosol Related Antibodies

5 Publications Citing Use of MCE Hydroxytyrosol

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

Biological Activity

Purity & Documentation

References

Customer Review

Hydroxytyrosol Related Antibodies

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

Hydroxytyrosol Related Classifications

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