2. Immunology/Inflammation
  3. Toll-like Receptor (TLR)
  4. Lipopolysaccharides, from P. gingivalis

Lipopolysaccharides, from P. gingivalis  (Synonyms: LPS, from Porphyromonas gingivalis)

Cat. No.: HY-D1056D
Handling Instructions Technical Support

Lipopolysaccharides, from P. gingivalis (LPS, from Porphyromonas gingivalis) are endotoxins and TLR4 activators extracted from Porphyromonas gingivalis (P. gingivalis) and are classified as S (smooth) type LPS. Lipopolysaccharides, from P. gingivalis possess the typical three-part structure: O-antigen, core oligosaccharide, and lipid A. Lipopolysaccharides, from P. gingivalis activate TLR-4 in immune cells and are important virulence factors in the mechanism of periodontal disease. Lipopolysaccharides, from P. gingivalis can be used in research related to periodontitis.

For research use only. We do not sell to patients.

Lipopolysaccharides, from P. gingivalis Chemical Structure

Lipopolysaccharides, from P. gingivalis Chemical Structure

Size Stock
1 mg Check price and availability
5 mg Check price and availability
10 mg Check price and availability

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

Lipopolysaccharides, from P. gingivalis Related Antibodies

Top Publications Citing Use of Products

View All Toll-like Receptor (TLR) Isoform Specific Products:

View All Isoforms
TLR3 TLR8 TLR9 TLR2 TLR4 TLR7 TLR1 TLR6

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

Lipopolysaccharides, from P. gingivalis (LPS, from Porphyromonas gingivalis) are endotoxins and TLR4 activators extracted from Porphyromonas gingivalis (P. gingivalis) and are classified as S (smooth) type LPS. Lipopolysaccharides, from P. gingivalis possess the typical three-part structure: O-antigen, core oligosaccharide, and lipid A. Lipopolysaccharides, from P. gingivalis activate TLR-4 in immune cells and are important virulence factors in the mechanism of periodontal disease. Lipopolysaccharides, from P. gingivalis can be used in research related to periodontitis[1][2][3][4].

IC50 & Target

TLR-4[2]
In Vitro

Note:
To maintain the integrity of LPS, it is recommended to store LPS solution in silanized containers. This is because LPS can adhere to plastics and certain types of glass, particularly at concentrations below 0.1 mg/mL. If the LPS concentration exceeds 1 mg/mL, this adsorption effect is relatively minimal. If using glass containers, ensure that the solution is thoroughly mixed for at least 30 minutes before use to redissolve any LPS that may have adsorbed to the tube walls.

LPS is the major toxic component of Gram-negative bacteria, capable of activating pathogen-associated molecular patterns (PAMP) of the immune system and inducing cellular secretion of migrasomes. LPS can be recognized by TLR4, activating the innate immune system, followed by promoting NF-κB activation and the production of pro-inflammatory cytokines, commonly used in experiments for the stimulation, activation, and differentiation of immune cells.
Different types of bacteria express LPS with varying structures and biological activities. LPS generally comes in two configurations: R (rough) type and S (smooth) type. S-type LPS contains a typical three-part structure: O-antigen (O-antigen) (serum-specific polysaccharides composed of repeating oligosaccharide units), core oligosaccharide (core) (C9-type non-repeating oligosaccharides), and lipid A (Lipid A) (the toxic component of LPS). The R type does not contain an O-antigen and expresses rough-type LPS. The lack of O-antigen can affect how immune cells recognize LPS.
Lipopolysaccharides, from P. gingivalis are classified as S-type LPS and can induce NF-κB activation in human periodontal ligament (PDL) cells[1].
Lipopolysaccharides, from P. gingivalis (pg-LPS) (1 μg/mL; 12-24 h) upregulate iNOS and COX-2 expression in RAW 264.7 cells and increase the levels of pro-inflammatory factors TNF-α, IL-1β, and IL-6[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Lipopolysaccharides, from P. gingivalis Related Antibodies

Western Blot Analysis[2]

Cell Line: RAW 264.7 cells
Concentration: 1 μg/mL
Incubation Time: 12-24 h
Result: Enhanced the expression levels of the pro-inflammatory cytokines TNF-α, IL-1β, and IL-6.
In Vivo

Induction of periodontitis model[2][3][4]
Background
LPS induces a periodontitis model by simulating bacterial infection, activating immune cells to release inflammatory mediators, triggering chronic inflammation, damaging periodontal tissues and affecting bone metabolism.
Specific Modeling Methods
C57BL/6 mice • BALB/c mice • 4-10 μg; 2-4 times • Injection into the oral periodontal tissues
Note
(1) Before inducing an animal model with LPS, relevant references should be consulted based on the experimental purpose, animal type, etc., and preliminary experiments should be conducted to determine the optimal experimental protocol.
(2) After LPS administration, the time points at which the peak levels of different inflammatory factors appear may vary. It is recommended to determine the experimental protocol according to references, and multiple time points should be selected for detection during preliminary experiments.
(3) LPS should be stored away from light and avoid repeated freezing and thawing.
(4) A certain concentration of DMSO can significantly inhibit the inflammatory response induced by LPS. It is recommended to dissolve LPS in PBS or ddH2O.
Modeling Indicators
The expressions of IL-1β, IL-6, TNF-α and so on in the gingival tissues increase.
HE staining of the gingival/alveolar bone tissues: infiltration of inflammatory cells, and the collagen fiber bundles are loosely distributed near the tissue-root interface.
The number of immune cells in the gingival tissues increases.
Correlated Product(s) Lipopolysaccharides, from E. coli O55:B5 (HY-D1056)
Opposite Product(s): Fucoidan (HY-132179)

Lipopolysaccharides, from P. gingivalis induce inflammatory responses in mouse and rat models of periodontitis[2][3].
Lipopolysaccharides, from P. gingivalis (pg-LPS) (1 μg/1 mL; 4 times; oral injection) can elicit tissue inflammatory responses in mice, promote the increase of pro-inflammatory factor levels, and recruit immune cells. The inflammatory response induced by Lipopolysaccharides, from P. gingivalis can be inhibited by Fucoidan (HY-132179) (5 mg/mL, 100 μL; twice daily for 3 days)[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Female BALB/c mice (6-week-old)[2]
Dosage: 1 μg in 1 μL sterile PBS
Administration: 4 times Buccal injection, via a microinjection and Hamilton N731 needle; with or without Fucoidan (HY-132179) (5 mg/mL with 2% carboxymethycellulose in 100 μL PBS), which was administered via oral topical application twice a day for 3 days after the last injection.
Result: Stimulated iNOS gene expression by 5.7-fold, which was significantly inhibited by fucoidan.
Elevated the the serum level of TNF, and resulted inflammation and inflammatory cell recruitment in mouse gingival tissue.
SMILES

[Lipopolysaccharides, from P. gingivalis]
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
References
  • No file chosen (Maximum size is: 1024 Kb)
  • If you have published this work, please enter the PubMed ID.
  • Your name will appear on the site.

Lipopolysaccharides, from P. gingivalis Related Classifications

  • Molarity Calculator

  • Dilution Calculator

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass   Concentration   Volume   Molecular Weight *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Lipopolysaccharides, from P. gingivalisLPS, from Porphyromonas gingivalisToll-like Receptor (TLR)Inhibitorinhibitorinhibit

Your Recently Viewed Products:

Inquiry Online

Your information is safe with us. * Required Fields.

Product Name

  

Requested Quantity *

Applicant Name *

 

Salutation

Email Address *

 

Phone Number *

Department

 

Organization Name *

City

State

Country or Region *

      

Remarks

Bulk Inquiry

Inquiry Information

Product Name:
Lipopolysaccharides, from P. gingivalis
Cat. No.:
HY-D1056D
Quantity:
MCE Japan Authorized Agent:

Customer Review

Thank You for Your Feedback!

Request for HNMR Report

Please fill out this form to request the QC report. We will send it to your Email address shortly.

Your information is safe with us. * Required Fields.

Product Name

  

Lot #

Applicant Name *

 

Email Address *

Phone Number

 

Department *

Organization Name *

 

Country or Region *

Remarks

SAFETY DATA SHEET (SDS)

English - EN (0 KB) Français - FR (0 KB) Deutsch - DE (0 KB) Norwegian - NO (0 KB) Español - ES (0 KB) Swedish - SV (0 KB)

Request for HNMR Report

We have received your request and will respond to you as soon as possible.