2. Immunology/Inflammation
  3. Toll-like Receptor (TLR)
  4. Lipopolysaccharides, from E. coli O127:B8

Lipopolysaccharides, from E. coli O127:B8  (Synonyms: LPS, from Escherichia coli (O127:B8))

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Lipopolysaccharides, from E. coli O127:B8 (LPS, from Escherichia coli (O127:B8)) are endotoxins and TLR4 activators extracted from Escherichia coli (E. coli O127:B8) and are classified as S (smooth) type LPS. Lipopolysaccharides, from E. coli O127:B8 possess the typical three-part structure: O-antigen, R3-type core oligosaccharide, and lipid A. Lipopolysaccharides, from E. coli O127:B8 activate TLR-4 in immune cells, can induce inflammatory responses and ileal contractility, and can be used to construct intestinal inflammation models.

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Lipopolysaccharides, from E. coli O127:B8 Chemical Structure

Lipopolysaccharides, from E. coli O127:B8 Chemical Structure

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Lipopolysaccharides, from E. coli O127:B8 Related Antibodies

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Description

Lipopolysaccharides, from E. coli O127:B8 (LPS, from Escherichia coli (O127:B8)) are endotoxins and TLR4 activators extracted from Escherichia coli (E. coli O127:B8) and are classified as S (smooth) type LPS. Lipopolysaccharides, from E. coli O127:B8 possess the typical three-part structure: O-antigen, R3-type core oligosaccharide, and lipid A. Lipopolysaccharides, from E. coli O127:B8 activate TLR-4 in immune cells, can induce inflammatory responses and ileal contractility, and can be used to construct intestinal inflammation models[1][2].

IC50 & Target

TLR-4[2]
In Vitro

Note:
To maintain the integrity of LPS, it is recommended to store LPS solution in silanized containers. This is because LPS can adhere to plastics and certain types of glass, particularly at concentrations below 0.1 mg/mL. If the LPS concentration exceeds 1 mg/mL, this adsorption effect is relatively minimal. If using glass containers, ensure that the solution is thoroughly mixed for at least 30 minutes before use to redissolve any LPS that may have adsorbed to the tube walls.

LPS is the major toxic component of Gram-negative bacteria, capable of activating pathogen-associated molecular patterns (PAMP) of the immune system and inducing cellular secretion of migrasomes. LPS can be recognized by TLR4, activating the innate immune system, followed by promoting NF-κB activation and the production of pro-inflammatory cytokines, commonly used in experiments for the stimulation, activation, and differentiation of immune cells.
Different types of bacteria express LPS with varying structures and biological activities. LPS generally comes in two configurations: R (rough) type and S (smooth) type. S-type LPS contains a typical three-part structure: O-antigen (O-antigen) (serum-specific polysaccharides composed of repeating oligosaccharide units), core oligosaccharide (core) (C9-type non-repeating oligosaccharides), and lipid A (Lipid A) (the toxic component of LPS). The R type does not contain an O-antigen and expresses rough-type LPS. The lack of O-antigen can affect how immune cells recognize LPS.
E. coli expresses four LPS serotypes: O111:B4, O55:B5, O127:B8, O128:B12. The LPS expressed by the O127:B8 strain is a common inflammatory inducer and can be used to construct models of intestinal and neonatal brain inflammation[1][2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Lipopolysaccharides, from E. coli O127:B8 Related Antibodies
In Vivo

Lipopolysaccharides, from E. coli O127:B8 (100 μg/kg; single dose) can induce a reduction in TLR4 mRNA expression in the muscle layer of rabbits but do not affect the mRNA levels in the mucosal layer. They also induce intestinal inflammation and limit spontaneous contractions as well as contractions caused by serotonin, acetylcholine, and KCl[1].
Lipopolysaccharides, from E. coli O127:B8 (20 μg/animal; single dose) cause preterm birth (delivery within 16 h post-injection) in pregnant CD1 mice, inducing downstream contraction and inflammatory signaling in the mouse myometrium and neonatal brain[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Appearance

Solid

Color

White to off-white

SMILES

[Lipopolysaccharides, from E. coli O127:B8]
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Room temperature in continental US; may vary elsewhere.
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4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility
In Vitro: 

H2O : 1 mg/mL (ultrasonic and warming and heat to 60°C)

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Lipopolysaccharides, from E. coli O127:B8 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Lipopolysaccharides, from E. coli O127:B8LPS, from Escherichia coli (O127:B8)Toll-like Receptor (TLR)Inhibitorinhibitorinhibit

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