2. Biochemical Assay Reagents

Biochemical Assay Reagents

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FAQ

Biochemical Assay Reagents refer to biological materials or organic compounds related to life science research, as well as reagents used in clinical diagnosis and medical research.

MedChemExpress (MCE) Biochemical Assay Reagents are available in a wide range of categories, including co-solvents, enzyme, enzyme substrates, cell assay reagents, indicators, etc. MCE aims to fully meet your experimental needs and help your research forward.

Biochemical Assay Reagents (7200):

Cat. No. Product Name CAS No. Purity Chemical Structure
  • HY-126388
    Trypsin Inhibitor, soybean 9035-81-8
    Trypsin Inhibitor, soybean is a potent and reversible inhibitor of trypsin.
    Trypsin Inhibitor, soybean
  • HY-116285
    n-Octyl β-D-glucopyranoside 29836-26-8 ≥98.0%
    n-Octyl-β-d-glucopyranoside is a non-ionic detergent, it can be widely used in the research of biotechnical, biochemical applications, solubilization and crystallization of membrane proteins. n-Octyl-β-d-glucopyranoside can completely inhibit cavitation-induced cell lysis in vitro.
    n-Octyl β-D-glucopyranoside
  • HY-D0714
    Tetrazolium Red 298-96-4 99.93%
    Tetrazolium Red (2,3,5-Triphenyltetrazolium chloride; TTC) is a not brain-penetrant, colorless, water-soluble dye that is reduced by mitochondrial enzymes to a deep red, water-insoluble compound (formazan) mainly in the mitochondria of living cells. Tetrazolium Red is used to observe the activity of dehydrogenase, and it turns colorless to red when exposed to hydrogen. Tetrazolium Red distinguishes between surviving and infarcted brain tissue after stroke. Tetrazolium Red has been used to stain heart tissue to measure the extent of acute lesions and also used to stain brain tissue to detect the size of the infarcted area. The absorption wavelength of Tetrazolium Red is 570 nm.
    Tetrazolium Red
  • HY-15926
    ONPG 369-07-3 99.91%
    ONPG is a colorimetric and spectrophotometric substrate for detection of β-galactosidase activity.
    ONPG
  • HY-D0719
    Fluorescein Diacetate 596-09-8 99.79%
    Fluorescein diacetate is a cell permeable esterase-substrate. Fluorescein diacetate can be used as a fluorogenic substrate for hGSTP1-1.
    Fluorescein Diacetate
  • HY-B0445A
    NAD sodium 20111-18-6
    NAD (β-Nicotinamide Adenine Dinucleotide) sodium is an analogue of NAD. NAD sodium can be reduced to β-nicotinamide adenine dinucleotide (NADH) during coupling with reactions which oxidize organic substrates. NAD sodium can be converted to β-nicotinamide adenine dinucleotide (NADH) and passes to the inside of mitochondria that indirectly generates ATP.
    NAD sodium
  • HY-15927
    4-Nitrophenyl β-D-glucopyranoside 2492-87-7 99.69%
    4-Nitrophenyl β-D-glucopyranoside is a chromogenic substrate for β-glucosidase. 4-Nitrophenyl β-D-glucopyranoside is converted to a colored product, p-nitrophenol that is easily detected spectrophotometrically at 405 nm when used in a β-glycosidase assay. 4-Nitrophenyl β-D-glucopyranoside is hydrolysed through intramolecular nucleophilic catalysis by the phosphate group in the 2-position. 4-Nitrophenyl β-D-glucopyranoside is promising for research of postmenopausal osteoporosis.
    4-Nitrophenyl β-D-glucopyranoside
  • HY-D0183
    ATP-polyamine-biotin 1800401-93-7 98.06%
    ATP-polyamine-biotin, the first cell-permeable ATP analogue, is an efficient kinase cosubstrate. ATP-polyamine-biotin promotes biotin labeling of kinase substrates in live cells.
    ATP-polyamine-biotin
  • HY-W014449
    4-Nitrophenyl butyrate 2635-84-9 ≥98.0%
    4-Nitrophenyl butyrate consists of butyric acid chains esterified with 4-nitrophenol groups, thus giving it a yellow color. This compound is commonly used as a substrate in enzyme assays to measure esterase and lipase activity. When these enzymes cleave the ester bond, the nitrophenol group is released and the color changes from yellow to orange. Thus, the rate of color change can be used to determine enzyme activity. In addition, 4-Nitrophenyl butyrate can also be used as organic synthesis reagent and dye intermediate.
    4-Nitrophenyl butyrate
  • HY-134426
    DL-β-Hydroxybutyryl coenzyme A lithium 103404-51-9 ≥98.0%
    DL-β-Hydroxybutyryl coenzyme A lithium is an intermediate in the fermentation of butyric acid and the metabolism of lysine and tryptophan, and is produced from β-hydroxybutyric acid by short-chain-CoA synthase.
    DL-β-Hydroxybutyryl coenzyme A lithium
  • HY-W011012
    Adenosine 5'-monophosphate disodium 4578-31-8
    Adenosine 5'-monophosphate disodium is an orally active purine nucleotide, and participates in ATP metabolism. Adenosine 5'-monophosphate disodium is also a ligand for adenosine 2B receptor. Adenosine 5'-monophosphate disodium can activate AMPK in skeletal muscle, and ameliorates insulin resistance and impaired glucose metabolism. Adenosine 5'-monophosphate disodium can be used for research of diabetes.
    Adenosine 5'-monophosphate disodium
  • HY-116022A
    4-Nitrophenyl phosphate disodium hexahydrate 333338-18-4 ≥98.0%
    4-Nitrophenyl phosphate (p-nitrophenyl phosphate) disodium hexahydrate is widely used as a small molecule phosphotyrosine-like substrate in activity assays for protein tyrosine phosphatases. 4-Nitrophenyl phosphate disodium hexahydrate is a colorless substrate that upon hydrolysis is converted to a yellow 4-nitrophenolate ion that can be monitored by absorbance at 405 nm.
    4-Nitrophenyl phosphate disodium hexahydrate
  • HY-W017386
    3-Methyl-2-oxovaleric acid sodium 3715-31-9 ≥98.0%
    3-Methyl-2-oxovaleric acid sodium is a degradation product from Isoleucine. 3-Methyl-2-oxovaleric acid sodium is a biomarker of mustard airway diseases (MADs) and uric acid stone.
    3-Methyl-2-oxovaleric acid sodium
  • HY-D0904
    Acetylthiocholine iodide 1866-15-5 ≥98.0%
    Acetylthiocholine iodide can be used as a substrate for certain enzymes, such as cholinesterase, etc., and can be used to determine the activity level of these enzymes. In addition, the compound is used in some medical research, for example in the fields of neuroscience and organ physiology.
    Acetylthiocholine iodide
  • HY-137855
    4-Methylumbelliferyl sulfate potassium 15220-11-8
    4-Methylumbelliferyl sulfate (potassium), a fluorescent substrate, is commonly used to detect sulfatase activity in biochemical and biomedical research. It consists of a sulfate group attached to a fluorescent molecule, which can be cleaved by sulfatase enzymes. Upon cleavage, 4-Methylumbelliferyl sulfate releases a highly fluorescent product that can be detected using fluorescence microscopy or spectroscopy. The use of 4-Methylumbelliferyl sulfate as a substrate for sulfatase enzymes allows accurate detection and quantification of these enzymes in a variety of biological samples.
    4-Methylumbelliferyl sulfate potassium
  • HY-128851B
    Coenzyme A sodium 55672-92-9 99.46%
    Coenzyme A (CoASH) sodium is a ubiquitous and essential cofactor, which is an acyl group carrier and carbonyl-activating group for the citric acid cycle and fatty acid metabolism. Coenzyme A plays a central role in the oxidation of pyruvate in the citric acid cycle and the metabolism of carboxylic acids, including short- and long-chain fatty acids.
    Coenzyme A sodium
  • HY-W016412
    Coenzyme Q0 605-94-7 ≥99.0%
    Coenzyme Q0 (CoQ0) is a potent, oral active ubiquinone compound can be derived from Antrodia cinnamomea. Coenzyme Q0 induces apoptosis and autophagy, suppresses of HER-2/AKT/mTOR signaling to potentiate the apoptosis and autophagy mechanisms. Coenzyme Q0 regulates NFκB/AP-1 activation and enhances Nrf2 stabilization in attenuation of inflammation and redox imbalance. Coenzyme Q0 has anti-angiogenic activity through downregulation of MMP-9/NF-κB and upregulation of HO-1 signaling.
    Coenzyme Q0
  • HY-141474
    Glutaryl coenzyme A lithium 103192-48-9 ≥99.0%
    Glutaryl coenzyme A lithium is a Glutaryl coenzyme A derivative. Glutaryl coenzyme A is an important endogenous metabolites. Glutaryl coenzyme A lithium can be used in HMG-CoA or Glutaryl-CoA related experiment.
    Glutaryl coenzyme A lithium
  • HY-W009274
    N-Acetylmuramic acid 10597-89-4 ≥99.0%
    N-acetylmuramic acid is a component of the bacterial cell wall peptidoglycan, essential for maintaining cell shape and integrity. N-acetylmuramic acid inhibits spore germination by inhibiting a coat-associated hexosaminidase and a core enzyme. N-acetylmuramic acid is required by Bacteroides forsythus for proliferation and the maintenance of its cell shape.
    N-Acetylmuramic acid
  • HY-15906
    AMPPD 122341-56-4 99.93%
    AMPPD (Lumi-Phos Plus; Lumigen PPD) is a chemiluminescent substrate for alkaline phosphatase (APase). AMPPD is hydrolyzed by APase to generate an unstable dioxetane intermediate, and the intermediate releases a chemiluminescent signal when it decomposes. The luminescent signal of AMPPD can be detected by highly sensitive equipment, thereby achieving quantitative analysis of the target molecule. AMPPD can be used in ultrasensitive enzyme-linked immunosorbent assays (such as quantitative detection of human tissue kininogen), chemiluminescent detection of proteins and nucleic acids, and other fields.
    AMPPD