Murrayafoline A

Cat. No.: HY-W100287 Purity: 99.73%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

Murrayafoline A is a carbazole alkaloid that can be extracted from Murraya tetramera. Murrayafoline A directly targets Specificity protein 1 (Sp1), thereby inhibiting NF-κB and MAPK signaling pathways. Murrayafoline A has anti-neuroinflammatory activity.

For research use only. We do not sell to patients.

Murrayafoline A Chemical Structure

CAS No. : 4532-33-6

Size	Price	Stock	Quantity
25 mg	USD 30	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 50	In-stock	Estimated Time of Arrival: December 31
100 mg		Get quote
200 mg		Get quote

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Biological Activity
Purity & Documentation
References
Customer Review

Description

Molecular Weight

211.26

Formula

C₁₄H₁₃NO

CAS No.

4532-33-6

Appearance

Solid

Color

White to off-white

SMILES

CC1=CC2=C(C(=C1)OC)NC3=CC=CC=C23

Structure Classification

Initial Source

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (473.35 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	4.7335 mL	23.6675 mL	47.3350 mL
5 mM	0.9467 mL	4.7335 mL	9.4670 mL
10 mM	0.4734 mL	2.3668 mL	4.7335 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (11.83 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (11.83 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.73%

Select Batch:

Data Sheet (277 KB) SDS (418 KB)

COA (256 KB) HNMR (242 KB) LCMS (91 KB)

Handling Instructions (2659 KB)

References

[1]. Li CH,et al. Natural carbazole alkaloid murrayafoline A displays potent anti-neuroinflammatory effect by directly targeting transcription factor Sp1 in LPS-induced microglial cells. Bioorg Chem. 2022 Dec;129:106178. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	4.7335 mL	23.6675 mL	47.3350 mL	118.3376 mL
	5 mM	0.9467 mL	4.7335 mL	9.4670 mL	23.6675 mL
	10 mM	0.4734 mL	2.3668 mL	4.7335 mL	11.8338 mL
	15 mM	0.3156 mL	1.5778 mL	3.1557 mL	7.8892 mL
	20 mM	0.2367 mL	1.1834 mL	2.3668 mL	5.9169 mL
	25 mM	0.1893 mL	0.9467 mL	1.8934 mL	4.7335 mL
	30 mM	0.1578 mL	0.7889 mL	1.5778 mL	3.9446 mL
	40 mM	0.1183 mL	0.5917 mL	1.1834 mL	2.9584 mL
	50 mM	0.0947 mL	0.4734 mL	0.9467 mL	2.3668 mL
	60 mM	0.0789 mL	0.3945 mL	0.7889 mL	1.9723 mL
	80 mM	0.0592 mL	0.2958 mL	0.5917 mL	1.4792 mL
	100 mM	0.0473 mL	0.2367 mL	0.4734 mL	1.1834 mL

Murrayafoline A Related Classifications

Neurological Disease Inflammation/Immunology

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Murrayafoline A4532-33-6NF-κBp38 MAPKNuclear factor-κBNuclear factor-kappaBSpecificity protein 1NeuroinflammationMAPKInhibitorinhibitorinhibit

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