2. Anti-infection Apoptosis Stem Cell/Wnt JAK/STAT Signaling Immunology/Inflammation
  3. Bacterial Parasite Antibiotic Fungal Apoptosis STAT Interleukin Related TNF Receptor
  4. Nifuratel

Nifuratel  (Synonyms: NF 113; SAP 113; Methylmercadone)

Cat. No.: HY-A0059 Purity: 99.77%
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Nifuratel (NF 113) is an orally active broad-spectrum antibiotic with antiprotozoal, antibacterial, anticancer and anti-inflammatory activities, and has good inhibitory effects on Candida and Trichomonas. Nifuratel is also a STAT3 inhibitor, which significantly inhibits the growth and proliferation of human gastric cancer cells and induces apoptosis. In addition, Nifuratel also inhibits mast cell-mediated antigen hypersensitivity reactions and can be used in the study of IgE-mediated allergic diseases.

For research use only. We do not sell to patients.

Nifuratel Chemical Structure

Nifuratel Chemical Structure

CAS No. : 4936-47-4

Size Price Stock Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 66 In-stock
Solution
10 mM * 1 mL in DMSO USD 66 In-stock
Solid
5 mg USD 38 In-stock
10 mg USD 60 In-stock
50 mg USD 96 In-stock
100 mg USD 120 In-stock
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Customer Review

Based on 3 publication(s) in Google Scholar

Other Forms of Nifuratel:

Nifuratel Related Antibodies

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STAT3 STAT5 STAT6 STAT1

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IL-1 IL-2 IL-3 IL-4 IL-5 IL-6 IL-8 IL-10 IL-12 IL-13 IL-15 IL-17 IL-23 IL-7 IL-33 IL-22

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TNFRSF1A/CD120a TNFRSF5/CD40 TNFRSF6/Fas/CD95 TNFRSF8/CD30 TNFRSF9/4-1BB/CD137 TNFRSF16/NGF Receptor/CD271 TNFRSF18/GITR/CD357

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Description

Nifuratel (NF 113) is an orally active broad-spectrum antibiotic with antiprotozoal, antibacterial, anticancer and anti-inflammatory activities, and has good inhibitory effects on Candida and Trichomonas. Nifuratel is also a STAT3 inhibitor, which significantly inhibits the growth and proliferation of human gastric cancer cells and induces apoptosis. In addition, Nifuratel also inhibits mast cell-mediated antigen hypersensitivity reactions and can be used in the study of IgE-mediated allergic diseases[1][2][3][4][5][6][7][8].

In Vitro

Nifuratel kills all Salmonella in RAW264.7 cells at 25 µM. Nifuratel kills all Salmonella in mouse J774.1 cells, avian HD11 cells, and human Hela cells at 50 µM, 100 µM, and 100 µM, respectively[4].
Nifuratel (0-100 µM; 15 h) completely inhibits the growth of SE C50041 strain in broth at a concentration of 100 µM[4].
Nifuratel (10-300 µM; 24 h) dose-dependently reduces colony formation in SGC7901 and BGC-823 cell lines with IC50 values ​​of 169.7 µM and 133.7 µM[5].
Nifuratel (75-300 µM; 24 h) blocks IL-6-induced activation of the STAT3 signaling pathway in SGC-7901 and BGC-823 cell lines[5].
Nifuratel inhibits antigen-induced degranulation in both RBL-2H3 cells and BMMCs in a concentration-dependent manner, with IC50 values ​​of 0.34 µM and 0.9 µM[6].
Nifuratel (0-3 µM; 30 min) dose-dependently inhibits the secretion of histamine from antigen-stimulated mast cells and the release of inflammatory factors TNF-α and IL-4 in antigen-stimulated RBL-2H3 cells, with IC50 values ​​of approximately 0.48 and 0.75 µM[6].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Nifuratel Related Antibodies

Cell Cycle Analysis[5]

Cell Line: SGC7901 and BGC-823 gastric cancer cell lines
Concentration: 75、100 and 300 µM
Incubation Time: 24 h
Result: Induced the arrest of gastric cancer cells in the G2/M phase of the cell cycle.

Western Blot Analysis[5]

Cell Line: SGC7901 and BGC-823 gastric cancer cell lines
Concentration: 75、100 and 300 µM
Incubation Time: 24 h
Result: Increased the expression of pro-apoptotic protein Bax and decreased the level of anti-apoptotic protein Bcl-2 in a dose-dependent manner.
Reduced phosphorylated STAT3 (P-STAT3) protein levels in SGC-7901 and BGC-823 cell lines at a dose of 300 μM.

Western Blot Analysis[6]

Cell Line: Antigen-stimulated RBL-2H3 cells
Concentration: 0、0.3、1、3 µM
Incubation Time: 30 min
Result: Inhibited the phosphorylation of Akt, ERK1/2, JNK and p38 in a concentration-dependent manner.
Almost completely inhibited Syk and Syk-mediated signaling pathways at 3 μM concentration.
In Vivo

Beauvericin (10-100 mg/kg; p.o.; oral administration on the day after IgE injection) inhibits IgE-mediated passive cutaneous anaphylaxis in Balb/c mice with an ED50 value of 22 mg/kg[6].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: IgE-dependent PCA mouse model[6]
Dosage: 10、30 and 100 mg/kg
Administration: Oral gavage (p.o.); IgE (50 ng) was injected subcutaneously into the ear of mice and then orally the next day.
Result: Reduced mast cell degranulation in a dose-dependent manner in the ear tissue of the IgE-dependent PCA mouse model.
Molecular Weight

285.28

Formula

C10H11N3O5S
CAS No.
Appearance

Solid

Color

Light yellow to yellow

SMILES

O=C1OC(CSC)CN1/N=C/C2=CC=C([N+]([O-])=O)O2
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 2 years
-20°C 1 year
Solvent & Solubility
In Vitro: 

DMSO : 50 mg/mL (175.27 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H2O : < 0.1 mg/mL (insoluble)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.5053 mL 17.5266 mL 35.0533 mL
5 mM 0.7011 mL 3.5053 mL 7.0107 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
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Concentration
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Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

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Volume (start)

V1

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C2

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Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.5 mg/mL (8.76 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% Corn Oil

    Solubility: ≥ 2.5 mg/mL (8.76 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 3.5053 mL 17.5266 mL 35.0533 mL 87.6332 mL
5 mM 0.7011 mL 3.5053 mL 7.0107 mL 17.5266 mL
10 mM 0.3505 mL 1.7527 mL 3.5053 mL 8.7633 mL
15 mM 0.2337 mL 1.1684 mL 2.3369 mL 5.8422 mL
20 mM 0.1753 mL 0.8763 mL 1.7527 mL 4.3817 mL
25 mM 0.1402 mL 0.7011 mL 1.4021 mL 3.5053 mL
30 mM 0.1168 mL 0.5842 mL 1.1684 mL 2.9211 mL
40 mM 0.0876 mL 0.4382 mL 0.8763 mL 2.1908 mL
50 mM 0.0701 mL 0.3505 mL 0.7011 mL 1.7527 mL
60 mM 0.0584 mL 0.2921 mL 0.5842 mL 1.4606 mL
80 mM 0.0438 mL 0.2191 mL 0.4382 mL 1.0954 mL
100 mM 0.0351 mL 0.1753 mL 0.3505 mL 0.8763 mL
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Nifuratel Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Nifuratel4936-47-4NF 113 SAP 113 MethylmercadoneNF113NF-113SAP113SAP 113SAP-113BacterialParasiteAntibioticFungalApoptosisSTATInterleukin RelatedTNF ReceptorILTumor Necrosis Factor ReceptorTNFRInhibitorinhibitorinhibit

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