2. PI3K/Akt/mTOR Cytoskeleton Cell Cycle/DNA Damage Metabolic Enzyme/Protease Apoptosis
  3. PI3K Akt Microtubule/Tubulin MMP Apoptosis
  4. PI3K/AKT-IN-2

PI3K/AKT-IN-2 (Compound 12c) is a PI3K and AKT inhibitor. PI3K/AKT-IN-2 blocks the epithelial-mesenchymal transition (EMT) and induces apoptosis. PI3K/AKT-IN-2 inhibits the polymerization of tubulin.

For research use only. We do not sell to patients.

PI3K/AKT-IN-2 Chemical Structure

PI3K/AKT-IN-2 Chemical Structure

CAS No. : 2684412-41-5

Size Price Stock Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 279 In-stock
Solution
10 mM * 1 mL in DMSO USD 279 In-stock
Solid
5 mg USD 195 In-stock
10 mg USD 315 In-stock
25 mg USD 630 In-stock
50 mg USD 1018 In-stock
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Customer Review

Based on 1 publication(s) in Google Scholar

PI3K/AKT-IN-2 Related Antibodies

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  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

PI3K/AKT-IN-2 (Compound 12c) is a PI3K and AKT inhibitor. PI3K/AKT-IN-2 blocks the epithelial-mesenchymal transition (EMT) and induces apoptosis. PI3K/AKT-IN-2 inhibits the polymerization of tubulin[1].

IC50 & Target

MMP-2

 

In Vitro

PI3K/AKT-IN-2 (Compound 12c) shows antiproliferative activity with IC50 values of 14.84 ± 1.53, 4.79 ± 0.71, 14.34 ± 0.57, 0.89 ± 0.023, >100 and 98.75 ± 1.59 µM against H460, MCF-7, A549, HGC-27, 293 T and GES-1 cells, respectively[1].
PI3K/AKT-IN-2 suppresses the expressions/activities of matrix metalloprotease (MMP)− 2, vimentin and up-regulation E-cadherin[1].
PI3K/AKT-IN-2 induces significant up-regulation of CDK1 proteins expression with a down-regulation of cyclin B1 and Cdc25C[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

PI3K/AKT-IN-2 Related Antibodies
Molecular Weight

651.45

Formula

C32H27BrO10
CAS No.
Appearance

Solid

Color

Light yellow to yellow

SMILES

O=C(O[C@@H]1[C@]2([H])[C@@](C(OC2)=O)([H])[C@H](C3=CC(OC)=C(OC)C(OC)=C3)C4=C1C=C5OCOC5=C4)/C=C/C(C6=CC=C(Br)C=C6)=O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (153.50 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.5350 mL 7.6752 mL 15.3504 mL
5 mM 0.3070 mL 1.5350 mL 3.0701 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Volume
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Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

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C2

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V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: 2.5 mg/mL (3.84 mM); Clear solution; Need ultrasonic

    This protocol yields a clear solution of 2.5 mg/mL.

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% Corn Oil

    Solubility: 2.5 mg/mL (3.84 mM); Clear solution; Need ultrasonic

    This protocol yields a clear solution of 2.5 mg/mL. If the continuous dosing period exceeds half a month, please choose this protocol carefully.

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

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(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 1.5350 mL 7.6752 mL 15.3504 mL 38.3759 mL
5 mM 0.3070 mL 1.5350 mL 3.0701 mL 7.6752 mL
10 mM 0.1535 mL 0.7675 mL 1.5350 mL 3.8376 mL
15 mM 0.1023 mL 0.5117 mL 1.0234 mL 2.5584 mL
20 mM 0.0768 mL 0.3838 mL 0.7675 mL 1.9188 mL
25 mM 0.0614 mL 0.3070 mL 0.6140 mL 1.5350 mL
30 mM 0.0512 mL 0.2558 mL 0.5117 mL 1.2792 mL
40 mM 0.0384 mL 0.1919 mL 0.3838 mL 0.9594 mL
50 mM 0.0307 mL 0.1535 mL 0.3070 mL 0.7675 mL
60 mM 0.0256 mL 0.1279 mL 0.2558 mL 0.6396 mL
80 mM 0.0192 mL 0.0959 mL 0.1919 mL 0.4797 mL
100 mM 0.0154 mL 0.0768 mL 0.1535 mL 0.3838 mL
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PI3K/AKT-IN-2 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

PI3K/AKT-IN-22684412-41-5PI3KAktMicrotubule/TubulinMMPApoptosisPhosphoinositide 3-kinasePKBProtein kinase BMatrix metalloproteinasesHGC-27MCF-7H460PTEN/PI3K/Aktgastric cancerInhibitorinhibitorinhibit

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