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  4. PRMT5:MEP50 PPI

PRMT5:MEP50 PPI is a novel PRMT5:MEP50 protein-protein interaction (PRMT5:MEP50 PPI) inhibitor, shows anti-tumor activity and anti-proliferative activity of lung and prostate cancer cells.

For research use only. We do not sell to patients.

PRMT5:MEP50 PPI Chemical Structure

PRMT5:MEP50 PPI Chemical Structure

CAS No. : 3031536-71-4

Size Price Stock Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 715 In-stock
Solution
10 mM * 1 mL in DMSO USD 715 In-stock
Solid
5 mg USD 650 In-stock
10 mg USD 1050 In-stock
25 mg USD 2150 In-stock
50 mg USD 3450 In-stock
100 mg USD 5400 In-stock
200 mg   Get quote  
500 mg   Get quote  

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Customer Review

Based on 1 publication(s) in Google Scholar

PRMT5:MEP50 PPI Related Antibodies

Top Publications Citing Use of Products

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

PRMT5:MEP50 PPI is a novel PRMT5:MEP50 protein-protein interaction (PRMT5:MEP50 PPI) inhibitor, shows anti-tumor activity and anti-proliferative activity of lung and prostate cancer cells[1].

Cellular Effect
Cell Line Type Value Description References
A549 IC50
447 nM
Compound: 17
Cytotoxicity against human A549 cells after 72 hrs by MTT assay
Cytotoxicity against human A549 cells after 72 hrs by MTT assay
[PMID: 36206451]
LNCaP IC50
430 nM
Compound: 17
Induction of cell death in human LNCaP cells incubated for 72 hrs by MTT assay
Induction of cell death in human LNCaP cells incubated for 72 hrs by MTT assay
[PMID: 36206451]
In Vitro

PRMT5:MEP50 PPI (0-10 μM; 72 h) inhibits the growth of prostate cancer cells[1].
PRMT5:MEP50 PPI (250-1000 nM; 72 h) decreases the expression of global arginine 3 residue of histone H4 (H4R3me2s) level in prostate cancer cells[1].
PRMT5:MEP50 PPI (500 nM; 72 h) de-represseses the PRMT5: MEP50-regulated IVL gene of prostate cancer cells[1].
PRMT5:MEP50 PPI treatment inhibits multiple PRMT5-regulated pathways critical to the survival and proliferation of lung and prostate cancer cells[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

PRMT5:MEP50 PPI Related Antibodies

Cell Viability Assay[1]

Cell Line: LNCaP cells
Concentration: 0-10 μM
Incubation Time: 72 h
Result: Showed IC50 value of 430.2 nM.

Western Blot Analysis[1]

Cell Line: LNCaP cells
Concentration: 250, 500 and 1000 nM
Incubation Time: 72 h
Result: Observed global arginine 3 residue of histone H4 (H4R3me2s) decreased by 65%.

RT-PCR[1]

Cell Line: LNCaP cells
Concentration: 500 nM
Incubation Time: 72 h
Result: De-repressesed the PRMT5: MEP50-regulated IVL gene without significantly altering the expression of the PRMT5: pICln-regulated AR gene.
Molecular Weight

430.46

Formula

C24H22N4O4
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

CCC1=NOC(C)=C1COC2=CC(C(NNC(C3=NC4=C(C=C3)C=CC=C4)=O)=O)=CC=C2
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility
In Vitro: 

DMSO : 200 mg/mL (464.62 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.3231 mL 11.6155 mL 23.2310 mL
5 mM 0.4646 mL 2.3231 mL 4.6462 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
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Concentration
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Volume
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Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

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Volume (start)

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C2

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Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 5 mg/mL (11.62 mM); Clear solution

    This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.3231 mL 11.6155 mL 23.2310 mL 58.0774 mL
5 mM 0.4646 mL 2.3231 mL 4.6462 mL 11.6155 mL
10 mM 0.2323 mL 1.1615 mL 2.3231 mL 5.8077 mL
15 mM 0.1549 mL 0.7744 mL 1.5487 mL 3.8718 mL
20 mM 0.1162 mL 0.5808 mL 1.1615 mL 2.9039 mL
25 mM 0.0929 mL 0.4646 mL 0.9292 mL 2.3231 mL
30 mM 0.0774 mL 0.3872 mL 0.7744 mL 1.9359 mL
40 mM 0.0581 mL 0.2904 mL 0.5808 mL 1.4519 mL
50 mM 0.0465 mL 0.2323 mL 0.4646 mL 1.1615 mL
60 mM 0.0387 mL 0.1936 mL 0.3872 mL 0.9680 mL
80 mM 0.0290 mL 0.1452 mL 0.2904 mL 0.7260 mL
100 mM 0.0232 mL 0.1162 mL 0.2323 mL 0.5808 mL
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PRMT5:MEP50 PPI Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

PRMT5:MEP50 PPI3031536-71-4Othersanti-tumor activityanti-proliferative activitylung cancer cellprostate cancer cellLNCaP cellInhibitorinhibitorinhibit

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