Pseudolaric Acid B

Name: Pseudolaric Acid B
Brand: MedChemExpress
SKU: HY-N6939
Rating: 4.5 (1 reviews)

Cat. No.: HY-N6939 Purity: 99.31%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

Pseudolaric Acid B is a diterpene isolated from the root of Pseudolarix kaempferi (pinaceae), has anti-cancer, antifungal, and antifertile activities, and shows immunosuppressive activity on T lymphocytes. Pseudolaric Acid B inhibits hepatitis B virus (HBV) secretion through apoptosis and cell cycle arrest. Pseudolaric Acid B induces autophagy.

For research use only. We do not sell to patients.

Pseudolaric Acid B Chemical Structure

CAS No. : 82508-31-4

Size	Price	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 133	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 133	In-stock	Estimated Time of Arrival: December 31
Solid
5 mg	USD 121	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 209	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 627	In-stock	Estimated Time of Arrival: December 31
100 mg	USD 1067	In-stock	Estimated Time of Arrival: December 31
200 mg		Get quote
500 mg		Get quote

or Bulk Inquiry

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 1 publication(s) in Google Scholar

Other Forms of Pseudolaric Acid B:

Pseudolaric Acid B (Standard) Get quote

1 Publications Citing Use of MCE Pseudolaric Acid B

•ACS Infect Dis. 2023 May 4. [Abstract]

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

Biological Activity
Purity & Documentation
References
Customer Review

Description

Pseudolaric Acid B is a diterpene isolated from the root of Pseudolarix kaempferi (pinaceae), has anti-cancer, antifungal, and antifertile activities, and shows immunosuppressive activity on T lymphocytes^[1]^[2]^[3]. Pseudolaric Acid B inhibits hepatitis B virus (HBV) secretion through apoptosis and cell cycle arrest. Pseudolaric Acid B induces autophagy^[4]^[5].

Cellular Effect

Cell Line	Type	Value	Description	References
A549	IC₅₀	0.3 μM Compound: pseudolaricacid B	Cytotoxicity against human A549 cells by MTT assay Cytotoxicity against human A549 cells by MTT assay	[PMID: 18651771]
A549	IC₅₀	0.3 μM Compound: pseudolaric acid B	Cytotoxicity against human A549 cells by SRB method Cytotoxicity against human A549 cells by SRB method	[PMID: 17323995]
A549	IC₅₀	0.3 μM Compound: PAB	Cytotoxicity against human A549 cells by SRB method Cytotoxicity against human A549 cells by SRB method	[PMID: 17253850]
A549	IC₅₀	0.86 μg/mL Compound: PAB	Cytotoxicity against human A549 cells after 24 hrs Cytotoxicity against human A549 cells after 24 hrs	[PMID: 15387644]
A549	IC₅₀	1.6 μM Compound: Pseudolaric acid B	Cytotoxicity against human A549 cells after 72 hrs by SRB assay Cytotoxicity against human A549 cells after 72 hrs by SRB assay	[PMID: 20038159]
Bel-7402	IC₅₀	> 100 μM Compound: 7	Cytotoxicity against human Bel7402 cells after 48 hrs by MTT assay Cytotoxicity against human Bel7402 cells after 48 hrs by MTT assay	[PMID: 17291040]
Bel-7402	IC₅₀	0.704 μM Compound: 1, pseudolaric acid B, PAB	Antiproliferative activity against human BEL-7402 cells by SRB assay Antiproliferative activity against human BEL-7402 cells by SRB assay	[PMID: 18078313]
Bel-7402	IC₅₀	1.3 μM Compound: Pseudolaric acid B	Cytotoxicity against human Bel7402 cells after 72 hrs by SRB assay Cytotoxicity against human Bel7402 cells after 72 hrs by SRB assay	[PMID: 20038159]
BGC-823	IC₅₀	> 100 μM Compound: 7	Cytotoxicity against human BGC823 cells after 48 hrs by MTT assay Cytotoxicity against human BGC823 cells after 48 hrs by MTT assay	[PMID: 17291040]
HCT-116	IC₅₀	0.136 μM Compound: 1, pseudolaric acid B, PAB	Antiproliferative activity against human HCT116 cells by SRB assay Antiproliferative activity against human HCT116 cells by SRB assay	[PMID: 18078313]
HeLa	IC₅₀	0.497 μM Compound: 1, pseudolaric acid B, PAB	Antiproliferative activity against human Hela cells by SRB assay Antiproliferative activity against human Hela cells by SRB assay	[PMID: 18078313]
HL-60	IC₅₀	0.235 μM Compound: 1, pseudolaric acid B, PAB	Antiproliferative activity against human HL60 cells by SRB assay Antiproliferative activity against human HL60 cells by SRB assay	[PMID: 18078313]
HL-60	IC₅₀	0.59 μM Compound: 7	Cytotoxicity against human HL60 cells after 48 hrs by MTT assay Cytotoxicity against human HL60 cells after 48 hrs by MTT assay	[PMID: 17291040]
HL-60	IC₅₀	4.2 μM Compound: Pseudolaric acid B	Cytotoxicity against human HL60 cells after 72 hrs by MTT assay Cytotoxicity against human HL60 cells after 72 hrs by MTT assay	[PMID: 20038159]
HMEC-1	IC₅₀	0.803 μM Compound: 1, pseudolaric acid B, PAB	Antiproliferative activity against HMEC1 cells by SRB assay Antiproliferative activity against HMEC1 cells by SRB assay	[PMID: 18078313]
MDA-MB-468	IC₅₀	0.596 μM Compound: 1, pseudolaric acid B, PAB	Antiproliferative activity against human MB-MDA-468 cells by SRB assay Antiproliferative activity against human MB-MDA-468 cells by SRB assay	[PMID: 18078313]
P388	IC₅₀	0.32 μg/mL Compound: PAB	Cytotoxicity against mouse P388 cells after 24 hrs Cytotoxicity against mouse P388 cells after 24 hrs	[PMID: 15387644]
P388	IC₅₀	0.74 μM Compound: pseudolaric acid B	Cytotoxicity against mouse P388 cells by MTT method Cytotoxicity against mouse P388 cells by MTT method	[PMID: 17323995]
P388	IC₅₀	3.7 μM Compound: PAB	Cytotoxicity against murine P388 cells by MTT method Cytotoxicity against murine P388 cells by MTT method	[PMID: 17253850]

Molecular Weight

432.46

Formula

C₂₃H₂₈O₈

CAS No.

82508-31-4

Appearance

Solid

Color

White to off-white

SMILES

O=C(C1=CC[C@@]([C@@](CC1)(OC(C)=O)[C@]2([H])[C@@](C)(/C=C/C=C(C(O)=O)\C)O3)(CC2)C3=O)OC

Structure Classification

Initial Source

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility

In Vitro:

DMSO : 50 mg/mL (115.62 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.3124 mL	11.5618 mL	23.1235 mL
5 mM	0.4625 mL	2.3124 mL	4.6247 mL
10 mM	0.2312 mL	1.1562 mL	2.3124 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (5.78 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.08 mg/mL (4.81 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.08 mg/mL (4.81 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 50% PEG300 50% Saline
Solubility: 10 mg/mL (23.12 mM); Suspended solution; Need ultrasonic

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.47%

Select Batch:

Data Sheet (274 KB) SDS (584 KB)

COA (249 KB) HNMR (215 KB) LCMS (120 KB)

Handling Instructions (2659 KB)

References

[1]. Zhou BN, et al. Some progress on the chemistry of natural bioactive terpenoids from Chinese medicinal plants. Mem Inst Oswaldo Cruz. 1991;86 Suppl 2:219-26. [Content Brief]

[2]. Wang WC, et al. [Endocrine activity of pseudolaric acids A and B and their effects on sex hormones, prostaglandins, uteri, and fetuses]. Zhongguo Yao Li Xue Bao. 1991 Mar;12(2):187-90. [Content Brief]

[3]. Wei N, et al. The immunosuppressive activity of pseudolaric acid B on T lymphocytes in vitro. Phytother Res. 2013 Jul;27(7):980-5. [Content Brief]

[4]. Yu J, et al. Pseudolaric acid B inhibits the secretion of hepatitis B virus. Oncol Rep. 2017 Jan;37(1):519-525. [Content Brief]

[5]. Yu J, et al. Pseudolaric acid B activates autophagy in MCF-7 human breast cancer cells to prevent cell death. Oncol Lett. 2016 Mar;11(3):1731-1737. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.3124 mL	11.5618 mL	23.1235 mL	57.8088 mL
	5 mM	0.4625 mL	2.3124 mL	4.6247 mL	11.5618 mL
	10 mM	0.2312 mL	1.1562 mL	2.3124 mL	5.7809 mL
	15 mM	0.1542 mL	0.7708 mL	1.5416 mL	3.8539 mL
	20 mM	0.1156 mL	0.5781 mL	1.1562 mL	2.8904 mL
	25 mM	0.0925 mL	0.4625 mL	0.9249 mL	2.3124 mL
	30 mM	0.0771 mL	0.3854 mL	0.7708 mL	1.9270 mL
	40 mM	0.0578 mL	0.2890 mL	0.5781 mL	1.4452 mL
	50 mM	0.0462 mL	0.2312 mL	0.4625 mL	1.1562 mL
	60 mM	0.0385 mL	0.1927 mL	0.3854 mL	0.9635 mL
	80 mM	0.0289 mL	0.1445 mL	0.2890 mL	0.7226 mL
	100 mM	0.0231 mL	0.1156 mL	0.2312 mL	0.5781 mL