2. Cytoskeleton Autophagy Apoptosis Metabolic Enzyme/Protease Protein Tyrosine Kinase/RTK Immunology/Inflammation
  3. HIF/HIF Prolyl-Hydroxylase Integrin Autophagy Apoptosis Endogenous Metabolite ROS Kinase COX
  4. Cucurbitacin B

Cucurbitacin B belongs to a class of highly oxidized tetracyclic triterpenoids and is oral active. Cucurbitacin B inhibits tumor cell growth, migration and invasion and cycle arrest, but induces cell apoptosis. Cucurbitacin B has potent anti-inflammatory, antioxidant, antiviral, hypoglycemic, hepatoprotective, neuroprotective activity.

For research use only. We do not sell to patients.

Cucurbitacin B Chemical Structure

Cucurbitacin B Chemical Structure

CAS No. : 6199-67-3

Size Price Stock Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 147 In-stock
Solution
10 mM * 1 mL in DMSO USD 147 In-stock
Solid
1 mg USD 57 In-stock
5 mg USD 120 In-stock
10 mg USD 204 In-stock
25 mg USD 408 In-stock
50 mg USD 720 In-stock
100 mg   Get quote  
200 mg   Get quote  

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 8 publication(s) in Google Scholar

Other Forms of Cucurbitacin B:

Cucurbitacin B Related Antibodies

Top Publications Citing Use of Products

View All HIF/HIF Prolyl-Hydroxylase Isoform Specific Products:

View All Isoforms
HIF-1α HIF

View All Integrin Isoform Specific Products:

View All Isoforms
αvβ1 αvβ3 αvβ5 αvβ6 αvβ8 α5β1 α1β1 α2β1 α4β1 α9β1 αIIbβ3 α4β7 αLβ2

View All COX Isoform Specific Products:

View All Isoforms
COX COX-1 COX-2 COX-3

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

Cucurbitacin B belongs to a class of highly oxidized tetracyclic triterpenoids and is oral active. Cucurbitacin B inhibits tumor cell growth, migration and invasion and cycle arrest, but induces cell apoptosis. Cucurbitacin B has potent anti-inflammatory, antioxidant, antiviral, hypoglycemic, hepatoprotective, neuroprotective activity[1][2][3][4][5].

Cellular Effect
Cell Line Type Value Description References
CHO IC50
1.99 μM
Compound: 2
Cytotoxicity against CHO cells by MTT assay
Cytotoxicity against CHO cells by MTT assay
[PMID: 30108825]
MCF7 IC50
12 μM
Compound: CuB
Cytotoxicity against human MCF7 cells assessed as reduction in cell viability incubated for 48 hrs by MTT assay
Cytotoxicity against human MCF7 cells assessed as reduction in cell viability incubated for 48 hrs by MTT assay
[PMID: 31620225]
MCF7 IC50
9.72 μM
Compound: CuB
Cytotoxicity against human MCF7 cells assessed as reduction in cell viability incubated for 48 hrs in presence of NQO1 inhibitor dicoumarol by MTT assay
Cytotoxicity against human MCF7 cells assessed as reduction in cell viability incubated for 48 hrs in presence of NQO1 inhibitor dicoumarol by MTT assay
[PMID: 31620225]
Vero IC50
0.04 μM
Compound: CuB
Cytotoxicity against African green monkey Vero cells assessed as reduction in cell viability incubated for 48 hrs by MTT assay
Cytotoxicity against African green monkey Vero cells assessed as reduction in cell viability incubated for 48 hrs by MTT assay
[PMID: 31620225]
In Vitro

Cucurbitacin B (up to 40 μM, 12-48 h) inhibits the cell growth and arrests cell cycle progression at the G2/M phase in CCA cell lines [2].
Cucurbitacin B (0.1, 0.3 and 1 μM) increases the level of total superoxide dismutase(T-SOD) and SOD-1, but decrease the reactive oxygen species (ROS) and malondialdehyde (MDA) in BY4741 yeast cells[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cucurbitacin B Related Antibodies

Cell Viability Assay[2]

Cell Line: CCA cell lines
Concentration: 0.1, 1 0.5, 1,5, 10, 20, 40 μM
Incubation Time: 24 and 48 h
Result: Decreased cell viability in a dose-dependent and time-dependent manner with the IC50 values of 13:44 μM for 24 h and 1.55 for 48 h.

Cell Cycle Analysis[2]

Cell Line: CCA cell lines
Concentration: 0.1, 1, 10 μM
Incubation Time: 24 h
Result: Arrested cell cycle progression at the G2/M phase.

Western Blot Analysis[2]

Cell Line: CCA cell lines
Concentration: 0.1, 1 0.5, 1,5, 10, 20, 40 μM
Incubation Time: 12 and 24 h
Result: Decreased the expression of Cyclin A, Cyclin D1, Cdc25A, but increased the level of p21.
In Vivo

Cucurbitacin B (5 mg/kg, oral administration 10day) B shows protective effects against carrageenan-induced prostatitis in rats[4].
Cucurbitacin B (20-50 mg/kg for i.p., 28 day) B afford a decline in AD symptoms and protects neurons against STZ-ICV toxicity that improved memory functions in rats[5].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Carrageenan-induced prostatic inflammation in rats[4]
Dosage: 5mg/kg/day for 10day
Administration: Oral administration
Result: Decreased the level of TNF-α, IL-1b, COX-2 and iNOS in prostatic tissues.
Animal Model: STZ-ICV rat prototype of AD-like dementia[5]
Dosage: 20, 50mg/kg/day for 28days
Administration: Intraperitoneal injection (i.p.)
Result: Decreased the TNF-α, IL-1β, MPO, iNOS, acetylcholinesterase, and glutamate levels, but increased gamma-aminobutyric acid.
Increase in viable neuron density in the cortex and hippocampus of rats.
Molecular Weight

558.70

Formula

C32H46O8
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

O[C@H](C1)[C@@]([C@@](C)(O)C(/C=C/C(OC(C)=O)(C)C)=O)([H])[C@](C2)(C)[C@]1(C)[C@]3([H])CC=C4C(C)(C)C([C@@H](O)C[C@@]4([H])[C@]3(C)C2=O)=O
Structure Classification
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : ≥ 100 mg/mL (178.99 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H2O : < 0.1 mg/mL (insoluble)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.7899 mL 8.9493 mL 17.8987 mL
5 mM 0.3580 mL 1.7899 mL 3.5797 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.5 mg/mL (4.47 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% Corn Oil

    Solubility: ≥ 2.5 mg/mL (4.47 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation

Purity: 99.91%

SDS (419 KB)

COA (252 KB) LCMS (93 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 1.7899 mL 8.9493 mL 17.8987 mL 44.7467 mL
5 mM 0.3580 mL 1.7899 mL 3.5797 mL 8.9493 mL
10 mM 0.1790 mL 0.8949 mL 1.7899 mL 4.4747 mL
15 mM 0.1193 mL 0.5966 mL 1.1932 mL 2.9831 mL
20 mM 0.0895 mL 0.4475 mL 0.8949 mL 2.2373 mL
25 mM 0.0716 mL 0.3580 mL 0.7159 mL 1.7899 mL
30 mM 0.0597 mL 0.2983 mL 0.5966 mL 1.4916 mL
40 mM 0.0447 mL 0.2237 mL 0.4475 mL 1.1187 mL
50 mM 0.0358 mL 0.1790 mL 0.3580 mL 0.8949 mL
60 mM 0.0298 mL 0.1492 mL 0.2983 mL 0.7458 mL
80 mM 0.0224 mL 0.1119 mL 0.2237 mL 0.5593 mL
100 mM 0.0179 mL 0.0895 mL 0.1790 mL 0.4475 mL
  • No file chosen (Maximum size is: 1024 Kb)
  • If you have published this work, please enter the PubMed ID.
  • Your name will appear on the site.

Cucurbitacin B Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Cucurbitacin B6199-67-3HIF/HIF Prolyl-HydroxylaseIntegrinAutophagyApoptosisEndogenous MetaboliteROS KinaseCOXHypoxia-inducible factorsHIFsHIF-PHCyclooxygenaseCCA cell linesBY4741 yeast cellsProstatitisAlzheimer's diseaseAntioxidantAntiviralHypoglycemicHepatoprotectiveInhibitorinhibitorinhibit

Your Recently Viewed Products:

Inquiry Online

Your information is safe with us. * Required Fields.

Product Name

  

Salutation

Applicant Name *

 

Email Address *

Phone Number *

 

Organization Name *

Department *

 

Requested quantity *

Country or Region *

      

Remarks

Bulk Inquiry

Inquiry Information

Product Name:
Cucurbitacin B
Cat. No.:
HY-N0416
Quantity:
MCE Japan Authorized Agent:

Customer Review

Thank You for Your Feedback!

Request for HNMR Report

Please fill out this form to request the QC report. We will send it to your Email address shortly.

Your information is safe with us. * Required Fields.

Product Name

  

Lot #

Applicant Name *

 

Email Address *

Phone Number

 

Department *

Organization Name *

 

Country or Region *

Remarks

SAFETY DATA SHEET (SDS)

English - EN (419 KB) Français - FR (419 KB) Deutsch - DE (419 KB) Norwegian - NO (419 KB) Español - ES (419 KB) Swedish - SV (419 KB)

Request for HNMR Report

We have received your request and will respond to you as soon as possible.