2. Metabolic Enzyme/Protease Anti-infection
  3. Mitochondrial Metabolism Reverse Transcriptase Aldehyde Dehydrogenase (ALDH)
  4. Daidzin

Daidzin  (Synonyms: Daidzoside; NPI-031D; Daidzein 7-O-glucoside)

Cat. No.: HY-N0018 Purity: 99.77%
SDS COA Handling Instructions

Molarity Calculator

Daidzin is an isoflavone with antioxidant, anticancer, and antiatherosclerotic activities. Daidzin is a potent and selective inhibitor of mitochondrial ALDH-2. Daidzin reduces ethanol consumption.

For research use only. We do not sell to patients.

Daidzin Chemical Structure

Daidzin Chemical Structure

CAS No. : 552-66-9

Size Price Stock Quantity
Free Sample (0.1 - 0.5 mg)   Apply Now  
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 55 In-stock
Solution
10 mM * 1 mL in DMSO USD 55 In-stock
Solid
5 mg USD 32 In-stock
10 mg USD 50 In-stock
50 mg USD 70 In-stock
100 mg USD 90 In-stock
200 mg USD 120 In-stock
500 mg USD 195 In-stock
1 g   Get quote  
5 g   Get quote  

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 5 publication(s) in Google Scholar

Other Forms of Daidzin:

Daidzin Related Antibodies

Top Publications Citing Use of Products

View All Aldehyde Dehydrogenase (ALDH) Isoform Specific Products:

View All Isoforms
ALDH1 ALDH2 ALDH3

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

Daidzin is an isoflavone with antioxidant, anticancer, and antiatherosclerotic activities. Daidzin is a potent and selective inhibitor of mitochondrial ALDH-2. Daidzin reduces ethanol consumption[1].

Cellular Effect
Cell Line Type Value Description References
HepG2 IC50
61.2 μM
Compound: 1
Anticancer activity against human HepG2 cells assessed as cell viability after 24 hrs by MTT assay
Anticancer activity against human HepG2 cells assessed as cell viability after 24 hrs by MTT assay
[PMID: 24974349]
In Vitro

Daidzin inhibits hamster and rat ALDH-2, with Kis of 0.082 and 0.052 μM respectively[1].
Daidzin (500 μM) shows antioxidant activity, measured by its ability to inhibit cytochrome c reduction by Phorbol myristic acetate (PMA)-stimulated neutrophils[3].
Daidzin (0-30 μM, 30 min) inhibits the formation of 5-HIAA (IC50 value of ≈2.7 μM), and increases the amounts of 5-HIAL and 5-HTOL in the incubation media of hamster liver mitochondrial[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Daidzin Related Antibodies
In Vivo

Daidzin (30 mg/kg, intragastrically) decreases blood alcohol levels (induced by 3 g ethanol/kg body weight), and shortens sleep time induced by Ethanol intoxication (7 g/kg by intubation) in fasted rats[3].
Daidzin (70 meq/hamster/day, i.p.) inhibits hamster ethanol intake by 62%[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Clinical Trial
Molecular Weight

416.38

Formula

C21H20O9
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

OC1=CC=C(C2=COC(C=C(O[C@H]3[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O3)C=C4)=C4C2=O)C=C1
Structure Classification
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : ≥ 200 mg/mL (480.33 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.4017 mL 12.0083 mL 24.0165 mL
5 mM 0.4803 mL 2.4017 mL 4.8033 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  0.5% CMC-Na/1% Tween-80 in Saline water

    Solubility: 39.6 mg/mL (95.11 mM); Suspended solution; Need ultrasonic

  • Protocol 2

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.08 mg/mL (5.00 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  15% Cremophor EL    85% Saline

    Solubility: 25 mg/mL (60.04 mM); Suspended solution; Need ultrasonic

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation

Purity: 99.77%

SDS (393 KB)

COA (252 KB) HNMR (191 KB) LCMS (110 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.4017 mL 12.0083 mL 24.0165 mL 60.0413 mL
5 mM 0.4803 mL 2.4017 mL 4.8033 mL 12.0083 mL
10 mM 0.2402 mL 1.2008 mL 2.4017 mL 6.0041 mL
15 mM 0.1601 mL 0.8006 mL 1.6011 mL 4.0028 mL
20 mM 0.1201 mL 0.6004 mL 1.2008 mL 3.0021 mL
25 mM 0.0961 mL 0.4803 mL 0.9607 mL 2.4017 mL
30 mM 0.0801 mL 0.4003 mL 0.8006 mL 2.0014 mL
40 mM 0.0600 mL 0.3002 mL 0.6004 mL 1.5010 mL
50 mM 0.0480 mL 0.2402 mL 0.4803 mL 1.2008 mL
60 mM 0.0400 mL 0.2001 mL 0.4003 mL 1.0007 mL
80 mM 0.0300 mL 0.1501 mL 0.3002 mL 0.7505 mL
100 mM 0.0240 mL 0.1201 mL 0.2402 mL 0.6004 mL
  • No file chosen (Maximum size is: 1024 Kb)
  • If you have published this work, please enter the PubMed ID.
  • Your name will appear on the site.

Daidzin Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Daidzin552-66-9Daidzoside NPI-031D Daidzein 7-O-glucosideMitochondrial MetabolismReverse TranscriptaseAldehyde Dehydrogenase (ALDH)isoflavone with antioxidantanticancerantiatherosclerotichamsterInhibitorinhibitorinhibit

Your Recently Viewed Products:

Inquiry Online

Your information is safe with us. * Required Fields.

Product Name

  

Salutation

Applicant Name *

 

Email Address *

Phone Number *

 

Organization Name *

Department *

 

Requested quantity *

Country or Region *

      

Remarks

Bulk Inquiry

Inquiry Information

Product Name:
Daidzin
Cat. No.:
HY-N0018
Quantity:
MCE Japan Authorized Agent:

Customer Review

Thank You for Your Feedback!

Request for HNMR Report

Please fill out this form to request the QC report. We will send it to your Email address shortly.

Your information is safe with us. * Required Fields.

Product Name

  

Lot #

Applicant Name *

 

Email Address *

Phone Number

 

Department *

Organization Name *

 

Country or Region *

Remarks

SAFETY DATA SHEET (SDS)

English - EN (393 KB) Français - FR (393 KB) Deutsch - DE (393 KB) Norwegian - NO (393 KB) Español - ES (393 KB) Swedish - SV (393 KB)

Request for HNMR Report

We have received your request and will respond to you as soon as possible.