2. Immunology/Inflammation Apoptosis
  3. COX Apoptosis
  4. Flurbiprofen

Flurbiprofen  (Synonyms: dl-Flurbiprofen)

Cat. No.: HY-10582 Purity: 99.98%
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Flurbiprofen (dl-Flurbiprofen) is a potent, orally active nonsteroidal anti-inflammatory agent (NSAIA/NSAID), with antipyretic and analgesic activities. Flurbiprofen is commonly used for the research of inflammatory diseases, including osteoarthritis and rheumatoid arthritis. Flurbiprofen is a non-selective cyclooxygenase (COX) inhibitor that can be used for the research of colorectal cancer.

For research use only. We do not sell to patients.

Flurbiprofen Chemical Structure

Flurbiprofen Chemical Structure

CAS No. : 5104-49-4

Size Price Stock Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 73 In-stock
Solution
10 mM * 1 mL in DMSO USD 73 In-stock
Solid
100 mg USD 66 In-stock
500 mg USD 92 In-stock
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Based on 1 publication(s) in Google Scholar

Other Forms of Flurbiprofen:

Flurbiprofen Related Antibodies

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COX COX-1 COX-2 COX-3

  • Biological Activity

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Description

Flurbiprofen (dl-Flurbiprofen) is a potent, orally active nonsteroidal anti-inflammatory agent (NSAIA/NSAID), with antipyretic and analgesic activities. Flurbiprofen is commonly used for the research of inflammatory diseases, including osteoarthritis and rheumatoid arthritis. Flurbiprofen is a non-selective cyclooxygenase (COX) inhibitor that can be used for the research of colorectal cancer[1][2][3].

IC50 & Target[1]

COX

 

COX-1

 

COX-2

 

In Vitro

Flurbiprofen (2-20 nM; 12-48 hours) significantly decreases SW620 cells proliferation in a concentration- and time-dependent manner[1].
Flurbiprofen (10 nM; 24 hours) decreases COX-2 expression[1].
Flurbiprofen (10 nM; 24 hours) inhibits the expression of inflammatory factors by inhibiting COX-2[1].
Flurbiprofen (10 nM; 24 hours) promotes the apoptosis of colorectal cancer cells by inhibiting COX-2[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Flurbiprofen Related Antibodies

Cell Proliferation Assay[1]

Cell Line: SW620 cells
Concentration: 2 nM, 4 nM, 10 nM, 20 nM
Incubation Time: 12 hours, 24 hours, 48 hours
Result: Inhibited colorectal cancer cell proliferation.

Western Blot Analysis[1]

Cell Line: SW620 cells
Concentration: 10 nM
Incubation Time: 24 hours
Result: Significantly decreased the protein and mRNA levels of COX-2.

RT-PCR[1]

Cell Line: SW620 cells
Concentration: 10 nM
Incubation Time: 24 hours
Result: Decreased COX-2 mRNA expression levels

Apoptosis Analysis[1]

Cell Line: SW620 cells
Concentration: 10 nM
Incubation Time: 24 hours
Result: Significantly decreased the expression of Bcl2 and significantly increased the expression of Bax and cleaved-caspase3, with no effect on total caspase-3.
In Vivo

Flurbiprofen (0.3-4.8 mg/kg; p.o.; 4-5 dosages) has acute anti-inflammatory in adrenalectomized rats[2].
Flurbiprofen (10 mg/kg; i.p.; daily; for 6 days) attenuates high-fat diet-induced obesity in mice[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Rat[2]
Dosage: 0.3 mg/kg, 0.6 mg/kg, 1.2 mg/kg, 2.4 mg/kg, 4.8 mg/kg
Administration: Oral administration, 4-5 dosages
Result: Inhibited the acute inflammation.
Clinical Trial
Molecular Weight

244.26

Formula

C15H13FO2
CAS No.
Appearance

Solid

Color

White to light yellow

SMILES

FC1=C(C2=CC=CC=C2)C=CC(C(C)C(O)=O)=C1
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Store at room temperature 3 years

In solvent -80°C 2 years
-20°C 1 year
Solvent & Solubility
In Vitro: 

DMSO : ≥ 100 mg/mL (409.40 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 4.0940 mL 20.4700 mL 40.9400 mL
5 mM 0.8188 mL 4.0940 mL 8.1880 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.5 mg/mL (10.23 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.5 mg/mL (10.23 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation

Purity: 99.98%

COA (246 KB) LCMS (264 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 4.0940 mL 20.4700 mL 40.9400 mL 102.3500 mL
5 mM 0.8188 mL 4.0940 mL 8.1880 mL 20.4700 mL
10 mM 0.4094 mL 2.0470 mL 4.0940 mL 10.2350 mL
15 mM 0.2729 mL 1.3647 mL 2.7293 mL 6.8233 mL
20 mM 0.2047 mL 1.0235 mL 2.0470 mL 5.1175 mL
25 mM 0.1638 mL 0.8188 mL 1.6376 mL 4.0940 mL
30 mM 0.1365 mL 0.6823 mL 1.3647 mL 3.4117 mL
40 mM 0.1023 mL 0.5117 mL 1.0235 mL 2.5587 mL
50 mM 0.0819 mL 0.4094 mL 0.8188 mL 2.0470 mL
60 mM 0.0682 mL 0.3412 mL 0.6823 mL 1.7058 mL
80 mM 0.0512 mL 0.2559 mL 0.5117 mL 1.2794 mL
100 mM 0.0409 mL 0.2047 mL 0.4094 mL 1.0235 mL
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Flurbiprofen Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Flurbiprofen5104-49-4dl-FlurbiprofenCOXApoptosisCyclooxygenasenonsteroidalanti-inflammatoryagent NSAIANSAIDosteoarthritisrheumatoidarthritisInhibitorinhibitorinhibit

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