Flurbiprofen (Synonyms: dl-Flurbiprofen)

Name: Flurbiprofen
Brand: MedChemExpress
SKU: HY-10582
Rating: 4.5 (1 reviews)

Cat. No.: HY-10582 Purity: 99.98%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

Flurbiprofen (dl-Flurbiprofen) is a potent, orally active nonsteroidal anti-inflammatory agent (NSAIA/NSAID), with antipyretic and analgesic activities. Flurbiprofen is commonly used for the research of inflammatory diseases, including osteoarthritis and rheumatoid arthritis. Flurbiprofen is a non-selective cyclooxygenase (COX) inhibitor that can be used for the research of colorectal cancer.

For research use only. We do not sell to patients.

Flurbiprofen Chemical Structure

CAS No. : 5104-49-4

Size	Price	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 73	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 73	In-stock	Estimated Time of Arrival: December 31
Solid
100 mg	USD 66	In-stock	Estimated Time of Arrival: December 31
500 mg	USD 92	In-stock	Estimated Time of Arrival: December 31
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5 g		Get quote

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Customer Review

Based on 1 publication(s) in Google Scholar

Other Forms of Flurbiprofen:

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View All COX Isoform Specific Products:

View All Isoforms

COX COX-1 COX-2 COX-3

Biological Activity
Purity & Documentation
References
Customer Review

Description

IC₅₀ & Target^[1]

COX

COX-1

COX-2

Cellular Effect

Cell Line	Type	Value	Description	References
Microglia	IC₅₀	100 nM Compound: Flurbiprofen	Antiinflammatory activity against LPS-induced rat Primary neonatal microglia assessed as inhibition in TXB2 generation preincubated for 20 mins followed by PMA-stimulation and measured after 70 mins by immunoassay Antiinflammatory activity against LPS-induced rat Primary neonatal microglia assessed as inhibition in TXB2 generation preincubated for 20 mins followed by PMA-stimulation and measured after 70 mins by immunoassay	[PMID: 32282204]
Microglia	IC₅₀	100 nM Compound: Flurbiprofen	Antineuroinflammatory activity in LPS-stimulated rat microglia cells assessed as inhibition of PMA-stimulated TXB2 release preincubated for 15 mins measured 70 mins after PMA challenge Antineuroinflammatory activity in LPS-stimulated rat microglia cells assessed as inhibition of PMA-stimulated TXB2 release preincubated for 15 mins measured 70 mins after PMA challenge	[PMID: 22153874]

In Vitro

Flurbiprofen (2-20 nM; 12-48 hours) significantly decreases SW620 cells proliferation in a concentration- and time-dependent manner^[1].
Flurbiprofen (10 nM; 24 hours) decreases COX-2 expression^[1].
Flurbiprofen (10 nM; 24 hours) inhibits the expression of inflammatory factors by inhibiting COX-2^[1].
Flurbiprofen (10 nM; 24 hours) promotes the apoptosis of colorectal cancer cells by inhibiting COX-2^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Proliferation Assay^[1]

Cell Line:	SW620 cells
Concentration:	2 nM, 4 nM, 10 nM, 20 nM
Incubation Time:	12 hours, 24 hours, 48 hours
Result:	Inhibited colorectal cancer cell proliferation.

Western Blot Analysis^[1]

Cell Line:	SW620 cells
Concentration:	10 nM
Incubation Time:	24 hours
Result:	Significantly decreased the protein and mRNA levels of COX-2.

RT-PCR^[1]

Cell Line:	SW620 cells
Concentration:	10 nM
Incubation Time:	24 hours
Result:	Decreased COX-2 mRNA expression levels

Apoptosis Analysis^[1]

Cell Line:	SW620 cells
Concentration:	10 nM
Incubation Time:	24 hours
Result:	Significantly decreased the expression of Bcl2 and significantly increased the expression of Bax and cleaved-caspase3, with no effect on total caspase-3.

In Vivo

Flurbiprofen (0.3-4.8 mg/kg; p.o.; 4-5 dosages) has acute anti-inflammatory in adrenalectomized rats^[2].
Flurbiprofen (10 mg/kg; i.p.; daily; for 6 days) attenuates high-fat diet-induced obesity in mice^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Rat^[2]
Dosage:	0.3 mg/kg, 0.6 mg/kg, 1.2 mg/kg, 2.4 mg/kg, 4.8 mg/kg
Administration:	Oral administration, 4-5 dosages
Result:	Inhibited the acute inflammation.

Clinical Trial

Molecular Weight

244.26

Formula

C₁₅H₁₃FO₂

CAS No.

5104-49-4

Appearance

Solid

Color

White to light yellow

SMILES

FC1=C(C2=CC=CC=C2)C=CC(C(C)C(O)=O)=C1

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Store at room temperature 3 years

In solvent	-80°C	2 years
	-20°C	1 year

Solvent & Solubility

In Vitro:

DMSO : ≥ 100 mg/mL (409.40 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	4.0940 mL	20.4700 mL	40.9400 mL
5 mM	0.8188 mL	4.0940 mL	8.1880 mL
10 mM	0.4094 mL	2.0470 mL	4.0940 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (10.23 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (10.23 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (10.23 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.98%

Select Batch:

Data Sheet (276 KB) SDS (479 KB)

COA (246 KB) LCMS (264 KB)

Handling Instructions (2659 KB)

References

[1]. Xiaobo Wang, et al. Flurbiprofen suppresses the inflammation, proliferation, invasion and migration of colorectal cancer cells via COX2. Oncol Lett. 2020 Nov; 20(5): 132. [Content Brief]

[2]. E M Glenn, et al. The pharmacology of 2-(2-fluoro-4-biphenylyl)propionic acid (flurbiprofen). A potent non-steroidal anti-inflammatory drug. Agents Actions. 1973 Nov;3(4):210-6. [Content Brief]

[3]. Hosoi, T., et al., Flurbiprofen ameliorated obesity by attenuating leptin resistance induced by endoplasmic reticulum stress. EMBO Mol Med, 2014. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	4.0940 mL	20.4700 mL	40.9400 mL	102.3500 mL
	5 mM	0.8188 mL	4.0940 mL	8.1880 mL	20.4700 mL
	10 mM	0.4094 mL	2.0470 mL	4.0940 mL	10.2350 mL
	15 mM	0.2729 mL	1.3647 mL	2.7293 mL	6.8233 mL
	20 mM	0.2047 mL	1.0235 mL	2.0470 mL	5.1175 mL
	25 mM	0.1638 mL	0.8188 mL	1.6376 mL	4.0940 mL
	30 mM	0.1365 mL	0.6823 mL	1.3647 mL	3.4117 mL
	40 mM	0.1023 mL	0.5117 mL	1.0235 mL	2.5587 mL
	50 mM	0.0819 mL	0.4094 mL	0.8188 mL	2.0470 mL
	60 mM	0.0682 mL	0.3412 mL	0.6823 mL	1.7058 mL
	80 mM	0.0512 mL	0.2559 mL	0.5117 mL	1.2794 mL
	100 mM	0.0409 mL	0.2047 mL	0.4094 mL	1.0235 mL

Flurbiprofen Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Flurbiprofen5104-49-4dl-FlurbiprofenCOXApoptosisCyclooxygenasenonsteroidalanti-inflammatoryagent NSAIANSAIDosteoarthritisrheumatoidarthritisInhibitorinhibitorinhibit

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Flurbiprofen (Synonyms: dl-Flurbiprofen)

Customer Review

Other Forms of Flurbiprofen:

Flurbiprofen Related Antibodies

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View All COX Isoform Specific Products:

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References

Customer Review

Flurbiprofen Related Antibodies

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Complete Stock Solution Preparation Table

Flurbiprofen Related Classifications

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