2. Cell Cycle/DNA Damage Autophagy Apoptosis
  3. DNA/RNA Synthesis Nucleoside Antimetabolite/Analog Autophagy Apoptosis
  4. Gemcitabine hydrochloride

Gemcitabine hydrochloride  (Synonyms: LY 188011 hydrochloride)

Cat. No.: HY-B0003 Purity: 99.89%
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Gemcitabine Hydrochloride (LY 188011 Hydrochloride) is a pyrimidine nucleoside analog antimetabolite and an antineoplastic agent. Gemcitabine Hydrochloride inhibits DNA synthesis and repair, resulting in autophagyand apoptosis.

For research use only. We do not sell to patients.

Gemcitabine hydrochloride Chemical Structure

Gemcitabine hydrochloride Chemical Structure

CAS No. : 122111-03-9

Size Price Stock Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 73 In-stock
Solution
10 mM * 1 mL in DMSO USD 73 In-stock
Solid
100 mg USD 66 In-stock
200 mg USD 79 In-stock
500 mg USD 112 In-stock
1 g USD 132 In-stock
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10 g   Get quote  

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Based on 149 publication(s) in Google Scholar

Other Forms of Gemcitabine hydrochloride:

Gemcitabine hydrochloride Related Antibodies

Top Publications Citing Use of Products

146 Publications Citing Use of MCE Gemcitabine hydrochloride

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Proliferation Assay

    Gemcitabine hydrochloride purchased from MedChemExpress. Usage Cited in: Naunyn Schmiedebergs Arch Pharmacol. 2019 May;392(5):615-622.  [Abstract]

    The expression level of p-JAK2, p-STAT3, Bcl-XL, and Mcl-1 in PANC-1 cells is detected by western blot analysis and is all down-regulated in the treatment groups.

    Gemcitabine hydrochloride purchased from MedChemExpress. Usage Cited in: Chem Biol Interact. 2018 Jun 25;290:44-51.  [Abstract]

    U2OS and MG-63 cells are treated with Gemcitabine, Licoricidin, or Gemcitabine+Licoricidin for 24 h, followed by the determination of active caspse-3 protein level using western blot. Cells without treatment are used as Control.

    Gemcitabine hydrochloride purchased from MedChemExpress. Usage Cited in: J Biol Chem. 2017 Jun 2;292(22):9136-9149.  [Abstract]

    The plate clone formation of SW480 and SW620 cells with Gemcitabine (8 nM in SW480 and 16 nM in SW620) and/or PX-12 (4 μM in SW480 and 8 μM in SW620).

    • Biological Activity

    • Purity & Documentation

    • References

    • Customer Review

    Description

    Gemcitabine Hydrochloride (LY 188011 Hydrochloride) is a pyrimidine nucleoside analog antimetabolite and an antineoplastic agent. Gemcitabine Hydrochloride inhibits DNA synthesis and repair, resulting in autophagyand apoptosis[1][2].

    IC50 & Target

    DNA synthesis[1]
    In Vitro

    Gemcitabine Hydrochloride (purchased from MedChem Express, 0.003-1 μM; 3 days) kills both mouse and human senescent cells effectively and potently[4].
    Gemcitabine Hydrochloride inhibits the growth of BxPC-3, Mia Paca-2, PANC-1, PL-45 and AsPC-1 cells with IC50s of 37.6, 42.9, 92.7, 89.3 and 131.4 nM, respectively[1].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Gemcitabine hydrochloride Related Antibodies

    Cell Viability Assay[4]

    Cell Line: Non-senescent and replication-induced senescent new born dermal fibroblasts (NBFs)
    Concentration: 0.003, 0.01, 0.03, 0.1, 0.3, 1 μM
    Incubation Time: 3 days
    Result: Killed replication-induced senescent NBFs for 3 days with 11.0% cell viability.
    In Vivo

    Gemcitabine Hydrochloride can be administered via endotracheal spray in rats without marked toxicity with a maximum tolerated dose of 4 mg/kg once a week for 9 weeks. The toxicity of Gemcitabine is lower via lung than oral administration at dosages of 2, 4, and 6 mg/kg[2].
    Treatment of the LSL-KrasG12D/+; LSL-Trp53R172H; Pdx-1-Cre mice with either Gemcitabine (50 mg/kg, i.p.) or the combination DMAPT/Gemcitabine Hydrochloride significantly increases the median survival time by more than 30 days compared to the placebo group (254.5 or 255 days vs. 217.5 days, respectively)[3].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
    Clinical Trial
    Molecular Weight

    299.66

    Formula

    C9H12ClF2N3O4
    CAS No.
    Appearance

    Solid

    Color

    White to off-white

    SMILES

    O[C@H]1C(F)(F)[C@H](N2C(N=C(N)C=C2)=O)O[C@@H]1CO.Cl
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage

    4°C, sealed storage, away from moisture and light

    *In solvent : -80°C, 1 year; -20°C, 6 months (sealed storage, away from moisture and light)

    Solvent & Solubility
    In Vitro: 

    DMSO : 62.5 mg/mL (208.57 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    H2O : 25 mg/mL (83.43 mM; Need ultrasonic)

    DMF : 2.5 mg/mL (8.34 mM; Need ultrasonic)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 3.3371 mL 16.6856 mL 33.3712 mL
    5 mM 0.6674 mL 3.3371 mL 6.6742 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months (sealed storage, away from moisture and light). When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
    =
    Concentration
    ×
    Volume
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    Molecular Weight *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

    C1

    ×
    Volume (start)

    V1

    =
    Concentration (final)

    C2

    ×
    Volume (final)

    V2

    In Vivo:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 2.08 mg/mL (6.94 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

      Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
    • Protocol 2

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

      Solubility: ≥ 2.08 mg/mL (6.94 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

      Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.

    For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  PBS

      Solubility: 25 mg/mL (83.43 mM); Clear solution; Need ultrasonic

    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Calculation results:
    Working solution concentration: mg/mL
    This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
    Purity & Documentation

    Purity: 99.93%

    COA (252 KB) LCMS (472 KB) Elemental Analysis Report (110 KB)

    Handling Instructions (2659 KB)
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months (sealed storage, away from moisture and light). When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMF / H2O / DMSO 1 mM 3.3371 mL 16.6856 mL 33.3712 mL 83.4279 mL
    5 mM 0.6674 mL 3.3371 mL 6.6742 mL 16.6856 mL
    H2O / DMSO 10 mM 0.3337 mL 1.6686 mL 3.3371 mL 8.3428 mL
    15 mM 0.2225 mL 1.1124 mL 2.2247 mL 5.5619 mL
    20 mM 0.1669 mL 0.8343 mL 1.6686 mL 4.1714 mL
    25 mM 0.1335 mL 0.6674 mL 1.3348 mL 3.3371 mL
    30 mM 0.1112 mL 0.5562 mL 1.1124 mL 2.7809 mL
    40 mM 0.0834 mL 0.4171 mL 0.8343 mL 2.0857 mL
    50 mM 0.0667 mL 0.3337 mL 0.6674 mL 1.6686 mL
    60 mM 0.0556 mL 0.2781 mL 0.5562 mL 1.3905 mL
    80 mM 0.0417 mL 0.2086 mL 0.4171 mL 1.0428 mL
    DMSO 100 mM 0.0334 mL 0.1669 mL 0.3337 mL 0.8343 mL

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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    Gemcitabine hydrochloride Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Gemcitabine122111-03-9LY 188011LY188011LY-188011DNA/RNA SynthesisNucleoside Antimetabolite/AnalogAutophagyApoptosisInhibitorinhibitorinhibit

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