2. Cell Cycle/DNA Damage Vitamin D Related/Nuclear Receptor Metabolic Enzyme/Protease Apoptosis Immunology/Inflammation
  3. PPAR TNF Receptor Interleukin Related Endogenous Metabolite
  4. Ginsenoside Rh1

Ginsenoside Rh1  (Synonyms: Prosapogenin A2; Sanchinoside B2; Sanchinoside Rh1)

Cat. No.: HY-N0604 Purity: ≥98.0%
COA Handling Instructions

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Ginsenoside Rh1 (Prosapogenin A2) inhibits the expression of PPAR-γ, TNF-α, IL-6, and IL-1β.

For research use only. We do not sell to patients.

Ginsenoside Rh1 Chemical Structure

Ginsenoside Rh1 Chemical Structure

CAS No. : 63223-86-9

Size Price Stock Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 56 In-stock
Solution
10 mM * 1 mL in DMSO USD 56 In-stock
Solid
5 mg USD 40 In-stock
10 mg USD 65 In-stock
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Customer Review

Based on 2 publication(s) in Google Scholar

Other Forms of Ginsenoside Rh1:

Ginsenoside Rh1 Related Antibodies

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  • Biological Activity

  • Protocol

  • Purity & Documentation

  • References

  • Customer Review

Description

Ginsenoside Rh1 (Prosapogenin A2) inhibits the expression of PPAR-γ, TNF-α, IL-6, and IL-1β.

IC50 & Target[1]

PPAR-γ

 

IL-6

 

IL-1β

 

In Vitro

The effect of Ginsenoside Rh1 is examined on adipogenesis in 3T3-L1 cells. Ginsenoside Rh1 potently inhibits the adipogenesis, as assessed by Oil-red O staining and lipid contents in 3T3-L1 adipocytes. Ginsenoside Rh1, at concentrations of 50 μM and 100 μM, inhibit the adipogenesis by 50% and 63%, respectively.The expression levels of adipocytespecific genes such as PPAR-γ, C/EBP-α, FAS, aFABP and some genes are examined during early phase of differentiation such as Pref-1, C/EBP-δ and Glucocorticoid receptor (GR). After the treatment with Ginsenoside Rh1 in 3T3-L1 cells, mRNA is extracted on 18 h and 24 h for Pref-1, C/EBP-δ and GR and day 8 for PPAR-γ, C/EBP-α, FAS, aFABP. Then, the expression profiles of adipocyte-specific genes are investigated by RT-PCR. PPAR-γ, C/EBP-α, FAS, and aFABP expressions are significantly increased in DMI-stimulated differentiated adipocyte compared to those of non-stimulated adipocyte cells. However, treatment with DMI in the presence of Ginsenoside Rh1 significantly suppresses the expression levels of PPAR-γ, C/EBP-α, FAS, and aFABP in a dose- dependent manner, whereas the expression levels of Pref-1, C/EBP-δ and GR are not affected[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Ginsenoside Rh1 Related Antibodies
In Vivo

When high-fat diet (HFD) fed mice for 8 weeks, body and epididymal fat weight gains are significantly increased compared to those of low-fat diet (LFD)-fed mice. However, when Ginsenoside Rh1 is treated in HFD-fed mice, body and epididymal fat weight gains are significantly decrease compared with those of the HFD-fed mice. TG, glucose, insulin, total cholesterol, and HDL levels in the blood are significantly increased in HFD-fed mice group compared to LFD-fed mice group. Treatment with Ginsenoside Rh1 in HFD-fed mice significantly lowers TG level alone[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Molecular Weight

638.87

Formula

C36H62O9
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

C[C@@]([C@@]12C)(C[C@H](O[C@]([C@@H]([C@@H](O)[C@@H]3O)O)([H])O[C@@H]3CO)[C@@]4([H])C5(C)C)[C@@](C[C@@H](O)[C@]1([H])[C@]([C@@](C)(O)CC/C=C(C)/C)([H])CC2)([H])[C@]4(CC[C@@H]5O)C
Structure Classification
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 2 years
-20°C 1 year
Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (156.53 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.5653 mL 7.8263 mL 15.6526 mL
5 mM 0.3131 mL 1.5653 mL 3.1305 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

  • Molarity Calculator

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
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Volume
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Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

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Volume (start)

V1

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Concentration (final)

C2

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Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.5 mg/mL (3.91 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.5 mg/mL (3.91 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation

Purity: ≥98.0%

COA (258 KB) HNMR (330 KB)

Handling Instructions (2659 KB)
References
Cell Assay
[1]

Mouse embryo fibroblasts 3T3-L1 cells are incubated in DMEM, containing 10% FBS and 1% AB, at 37°C and 5.6% CO2 atmosphere. To induce differentiation, two days after confluence, preadipocytes (designated day 0) are cultured in the differentiation medium (DM), which is consisted of DMEM, 10% FBS, 1% AB, and DMI (0.28 unit/mL insulin, 0.5 mM Isobutylmethylxanthine and 1 μM Dexamethasone) for 2 d in the presence or absence of 50 μM and 100 μM of Ginsenoside Rh1, and switched to DM containing 10% FBS and 10 μg/mL insulin and then changed to DMEM medium with 10% FBS for every 2 d[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Animal Administration
[1]

Mice[1]
Male C57BL/6J mice are separated into 3 groups, LFD, HFD and HFDRh1. Each group is consisted of ten mice. LFD group fed LFD for 8 weeks. HFD group fed HFD for 8 weeks. HFDRh1 group fed HFD diet for 4 weeks and then simultaneously treated with HFD and 20 mg/kg/d Ginsenoside Rh1, which is orally administrated. Weight and food intake of mice are measured daily. After finishing treatment for 4 weeks, blood and epididymal fats are collected for further analysis.

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 1.5653 mL 7.8263 mL 15.6526 mL 39.1316 mL
5 mM 0.3131 mL 1.5653 mL 3.1305 mL 7.8263 mL
10 mM 0.1565 mL 0.7826 mL 1.5653 mL 3.9132 mL
15 mM 0.1044 mL 0.5218 mL 1.0435 mL 2.6088 mL
20 mM 0.0783 mL 0.3913 mL 0.7826 mL 1.9566 mL
25 mM 0.0626 mL 0.3131 mL 0.6261 mL 1.5653 mL
30 mM 0.0522 mL 0.2609 mL 0.5218 mL 1.3044 mL
40 mM 0.0391 mL 0.1957 mL 0.3913 mL 0.9783 mL
50 mM 0.0313 mL 0.1565 mL 0.3131 mL 0.7826 mL
60 mM 0.0261 mL 0.1304 mL 0.2609 mL 0.6522 mL
80 mM 0.0196 mL 0.0978 mL 0.1957 mL 0.4891 mL
100 mM 0.0157 mL 0.0783 mL 0.1565 mL 0.3913 mL
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Ginsenoside Rh1 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Ginsenoside Rh163223-86-9Prosapogenin A2 Sanchinoside B2 Sanchinoside Rh1Ginsenoside Rh 1Ginsenoside Rh-1Prosapogenin A 2Prosapogenin A-2Sanchinoside B2Sanchinoside B 2Sanchinoside B-2Sanchinoside Rh1Sanchinoside Rh 1Sanchinoside Rh-1PPARTNF ReceptorInterleukin RelatedEndogenous MetabolitePeroxisome proliferator-activated receptorsTumor Necrosis Factor ReceptorTNFRILInhibitorinhibitorinhibit

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