Indole-3-carbinol (Synonyms: Indole-3-methanol)

Name: Indole-3-carbinol
Brand: MedChemExpress
SKU: HY-N0170
Rating: 5.0 (11 reviews)

Cat. No.: HY-N0170 Purity: 98.87%: FAQs
Data Sheet SDS COA Handling Instructions

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Indole-3-carbinol (I3C) inhibits NF-κB activity and also is an Aryl hydrocarbon receptor (AhR) agonist, and an inhibitor of WWP1 (WW domain-containing ubiquitin E3 ligase 1).

For research use only. We do not sell to patients.

Indole-3-carbinol Chemical Structure

CAS No. : 700-06-1

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10 mM * 1 mL in DMSO ready for reconstitution	USD 33	In-stock	Estimated Time of Arrival: December 31
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Based on 11 publication(s) in Google Scholar

9 Publications Citing Use of MCE Indole-3-carbinol

IHC

RT-PCR

: Indole-3-carbinol purchased from MedChemExpress. Usage Cited in: Molecules. 2018 Oct 11;23(10). pii: E2600. [Abstract]; The cells are cultured with I3C (50 μM) or 3MC (1 μM) in the presence of IPRN (20 μM). The IPRN-induced inhibition of nucleocytoplasmic shuttling of AhR is suppressed by I3C and 3MC.

: Indole-3-carbinol purchased from MedChemExpress. Usage Cited in: Molecules. 2018 Oct 11;23(10). pii: E2600. [Abstract]; The cells are cultured with I3C (50 μM) or 3MC (1 μM) in the presence of IPRN (20 μM). The mRNA expression of CYP1A1 is increased after the cotreatment of I3C and 3MC.

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Biological Activity
Protocol
Purity & Documentation
References
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Description

Indole-3-carbinol (I3C) inhibits NF-κB activity and also is an Aryl hydrocarbon receptor (AhR) agonist, and an inhibitor of WWP1 (WW domain-containing ubiquitin E3 ligase 1).

IC₅₀ & Target^[1]^[2]

NF-κB

AhR

Human Endogenous Metabolite

Cellular Effect

Cell Line	Type	Value	Description	References
U-251	EC₅₀	390 μM Compound: 3; I3C	Cytotoxicity against human U251 cells assessed as decrease in cell proliferation after 72 hrs by SRB assay Cytotoxicity against human U251 cells assessed as decrease in cell proliferation after 72 hrs by SRB assay	[PMID: 28256372]
U-87MG ATCC	EC₅₀	290 μM Compound: 3; I3C	Cytotoxicity against human U87 cells assessed as decrease in cell proliferation after 72 hrs by SRB assay Cytotoxicity against human U87 cells assessed as decrease in cell proliferation after 72 hrs by SRB assay	[PMID: 28256372]
U-87MG ATCC	IC₅₀	526 μM Compound: 1, I3C	Antiproliferative activity against human U87MG cells assessed as inhibition of cell growth after 48 hrs by MTS assay Antiproliferative activity against human U87MG cells assessed as inhibition of cell growth after 48 hrs by MTS assay	[PMID: 23419325]

In Vitro

It is found that Indole-3-carbinol (I3C) inhibits the proliferation of THP-1 cells in a dose- and time dependent manner with minimal toxicity over normal monocytes. The AhR target genes (CYP1A1, IL1β) are overexpressed upon Indole-3-carbinol treatment (p<0.05 to p<0.001). The antiproliferative effects of Indole-3-carbinol are in association with programing cell death. Indole-3-carbinol downregulates BCL2 and upregulates FasR in THP-1 cells (p<0.05 to p<0.001). G1 cell cycle arrest is also observed using flow cytometry. G1-acting cell cycle genes (P21, P27 and P53) are overexpressed (p<0.05 to p<0.001), while CDK2 is downregulated upon Indole-3-carbinol treatment (p<0.01 to p<0.001)^[1].Indole-3-carbinol suppresses NF-κB activity and stimulates the p53 pathway in pre-B acute lymphoblastic leukemia cells^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

147.17

Formula

C₉H₉NO

CAS No.

700-06-1

Appearance

Solid

Color

White to off-white

SMILES

OCC1=CNC2=C1C=CC=C2

Structure Classification

Initial Source

Endogenous metabolite

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (679.49 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	6.7949 mL	33.9743 mL	67.9486 mL
5 mM	1.3590 mL	6.7949 mL	13.5897 mL
10 mM	0.6795 mL	3.3974 mL	6.7949 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (16.99 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (16.99 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (16.99 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 98.87%

Select Batch:

Data Sheet (279 KB) SDS (393 KB)

COA (251 KB) RP-HPLC (194 KB)

Handling Instructions (2659 KB)

References

[1]. Mohammadi S, et al. Indole-3-carbinol induces G1 cell cycle arrest and apoptosis through aryl hydrocarbon receptor in THP-1 monocytic cell line.J Recept Signal Transduct Res. 2017 Oct;37(5):506-514. [Content Brief]

[2]. Safa M, et al. Indole-3-carbinol suppresses NF-κB activity and stimulates the p53 pathway in pre-B acute lymphoblastic leukemia cells. Tumour Biol. 2015 May;36(5):3919-30. [Content Brief]

[3]. Lee YR, et al. Reactivation of PTEN tumor suppressor for cancer treatment through inhibition of a MYC-WWP1 inhibitory pathway. Science. 2019 May 17;364(6441). pii: eaau0159. [Content Brief]

Cell Assay
^[1]

THP-1 cells are cultured in RPMI 1640 supplemented with 10% FBS, 100 U/mL penicillin, 100 mg/mL streptomycin and 2 mM Glutamax in a fully humidified atmosphere with 5% CO₂. Cells (2-5×10⁵ mL^-1) are seeded in six well plates followed by resuspension in complete growth media. THP-1 monocyte cells are then treated with 10 ng/mL phorbol 12-myristate 13-acetate as a tumor promoter to induce stable differentiation into attaching macrophage-like cells and overexpression of AhR. The cells are then treated with varying concentrations of Indole-3-carbinol (1, 10 and 100 μM, and 1 mM). THP-1 cells and enriching normal monocytes are seeded at 5×10⁴ cells/well in 24-well plate with different concentrations of Indole-3-carbinol and observed for 24 and 48 h followed by MTT assay. The cells are incubated in triplicates in a final volume of 200 mL of Phenol Red free RPMI 1640 for 20 h . An aliquot of 20 mL of MTT solution (5 mg/mL) is added to each well and incubated for 4 h. Formazan crystals are formed. An amount of 300 mL DMSO is then added to each well as a cell lysis solution. Percentage of cell viability is assessed by spectrophotometry at 570 nm^[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References

[1]. Mohammadi S, et al. Indole-3-carbinol induces G1 cell cycle arrest and apoptosis through aryl hydrocarbon receptor in THP-1 monocytic cell line.J Recept Signal Transduct Res. 2017 Oct;37(5):506-514. [Content Brief]

[2]. Safa M, et al. Indole-3-carbinol suppresses NF-κB activity and stimulates the p53 pathway in pre-B acute lymphoblastic leukemia cells. Tumour Biol. 2015 May;36(5):3919-30. [Content Brief]

[3]. Lee YR, et al. Reactivation of PTEN tumor suppressor for cancer treatment through inhibition of a MYC-WWP1 inhibitory pathway. Science. 2019 May 17;364(6441). pii: eaau0159. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	6.7949 mL	33.9743 mL	67.9486 mL	169.8716 mL
	5 mM	1.3590 mL	6.7949 mL	13.5897 mL	33.9743 mL
	10 mM	0.6795 mL	3.3974 mL	6.7949 mL	16.9872 mL
	15 mM	0.4530 mL	2.2650 mL	4.5299 mL	11.3248 mL
	20 mM	0.3397 mL	1.6987 mL	3.3974 mL	8.4936 mL
	25 mM	0.2718 mL	1.3590 mL	2.7179 mL	6.7949 mL
	30 mM	0.2265 mL	1.1325 mL	2.2650 mL	5.6624 mL
	40 mM	0.1699 mL	0.8494 mL	1.6987 mL	4.2468 mL
	50 mM	0.1359 mL	0.6795 mL	1.3590 mL	3.3974 mL
	60 mM	0.1132 mL	0.5662 mL	1.1325 mL	2.8312 mL
	80 mM	0.0849 mL	0.4247 mL	0.8494 mL	2.1234 mL
	100 mM	0.0679 mL	0.3397 mL	0.6795 mL	1.6987 mL

Indole-3-carbinol Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Indole-3-carbinol700-06-1Indole-3-methanolNF-κBAryl Hydrocarbon ReceptorE1/E2/E3 EnzymeEndogenous MetaboliteNuclear factor-κBNuclear factor-kappaBAhRE1 activating enzymeE2 conjugating enzymeE3 ligating enzymeUbiquitin activating enzymeUbiquitin conjugating enzymeUbiquitin ligaseInhibitorinhibitorinhibit

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Indole-3-carbinol (Synonyms: Indole-3-methanol)

Customer Review

Indole-3-carbinol Related Antibodies

9 Publications Citing Use of MCE Indole-3-carbinol

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View All NF-κB Isoform Specific Products:

Biological Activity

Protocol

Purity & Documentation

References

Customer Review

Indole-3-carbinol Related Antibodies

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

Indole-3-carbinol Related Classifications

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