Indotecan (Synonyms: LMP-400; NSC-724998)

Name: Indotecan
Brand: MedChemExpress
SKU: HY-18351
Rating: 4.5 (1 reviews)

Cat. No.: HY-18351 Purity: ≥98.0%: FAQs
Data Sheet SDS COA Handling Instructions

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Indotecan (LMP-400), an indenoisoquinoline derivative, is a potent Topoisomerase I inhibitor, with IC₅₀s of 300, 1200, 560 nM for P388, HCT116, MCF-7 cell lines, respectively. Indotecan prevents the relaxation of supercoiled DNA and can be used for the research of visceral leishmaniasis.

For research use only. We do not sell to patients.

Indotecan Chemical Structure

CAS No. : 915303-09-2

Size	Price	Stock	Quantity
1 mg	USD 80	In-stock	Estimated Time of Arrival: December 31
5 mg	USD 160	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 256	In-stock	Estimated Time of Arrival: December 31
25 mg	USD 450	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 720	In-stock	Estimated Time of Arrival: December 31
100 mg	USD 1150	In-stock	Estimated Time of Arrival: December 31
200 mg		Get quote
500 mg		Get quote

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Customer Review

Based on 1 publication(s) in Google Scholar

Other Forms of Indotecan:

Indotecan hydrochloride Get quote

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Topoisomerase Topo I Topo II

Biological Activity
Purity & Documentation
References
Customer Review

Description

IC₅₀ & Target^[1]

Topoisomerase I

Cellular Effect

Cell Line	Type	Value	Description	References
DU-145	GI₅₀	0.155 μM Compound: 6; MP400; NSC 724998	Antiproliferative activity in human DU145 cells assessed as cell growth inhibition after 48 hrs by SRB assay Antiproliferative activity in human DU145 cells assessed as cell growth inhibition after 48 hrs by SRB assay	[PMID: 27097152]
DU-145	GI₅₀	0.155 μM Compound: 6, indotecan	Antiproliferative activity against human DU145 cells by SRB assay Antiproliferative activity against human DU145 cells by SRB assay	[PMID: 21710981]
HCT-116	GI₅₀	1.15 μM Compound: 6; MP400; NSC 724998	Antiproliferative activity in human HCT116 cells assessed as cell growth inhibition after 48 hrs by SRB assay Antiproliferative activity in human HCT116 cells assessed as cell growth inhibition after 48 hrs by SRB assay	[PMID: 27097152]
HCT-116	GI₅₀	1.15 μM Compound: 6, indotecan	Antiproliferative activity against human HCT116 cells by SRB assay Antiproliferative activity against human HCT116 cells by SRB assay	[PMID: 21710981]
HOP-62	GI₅₀	1.78 μM Compound: 6; MP400; NSC 724998	Antiproliferative activity in human HOP62 cells assessed as cell growth inhibition after 48 hrs by SRB assay Antiproliferative activity in human HOP62 cells assessed as cell growth inhibition after 48 hrs by SRB assay	[PMID: 27097152]
HOP-62	GI₅₀	1.78 μM Compound: 6, indotecan	Antiproliferative activity against human HOP62 cells by SRB assay Antiproliferative activity against human HOP62 cells by SRB assay	[PMID: 21710981]
MCF7	GI₅₀	0.37 μM Compound: 6, indotecan	Antiproliferative activity against human MCF7 cells by SRB assay Antiproliferative activity against human MCF7 cells by SRB assay	[PMID: 21710981]
MCF7	GI₅₀	0.374 μM Compound: 6; MP400; NSC 724998	Antiproliferative activity in human MCF7 cells assessed as cell growth inhibition after 48 hrs by SRB assay Antiproliferative activity in human MCF7 cells assessed as cell growth inhibition after 48 hrs by SRB assay	[PMID: 27097152]
OVCAR-3	GI₅₀	74.1 μM Compound: 6, indotecan	Antiproliferative activity against human OVCAR3 cells by SRB assay Antiproliferative activity against human OVCAR3 cells by SRB assay	[PMID: 21710981]
SF-539	GI₅₀	0.04 μM Compound: 6; MP400; NSC 724998	Antiproliferative activity in human SF539 cells assessed as cell growth inhibition after 48 hrs by SRB assay Antiproliferative activity in human SF539 cells assessed as cell growth inhibition after 48 hrs by SRB assay	[PMID: 27097152]
SF-539	GI₅₀	0.04 μM Compound: 6, indotecan	Antiproliferative activity against human SF539 cells by SRB assay Antiproliferative activity against human SF539 cells by SRB assay	[PMID: 21710981]
SN12C	GI₅₀	0.813 μM Compound: 6; MP400; NSC 724998	Antiproliferative activity in human SN12C cells assessed as cell growth inhibition after 48 hrs by SRB assay Antiproliferative activity in human SN12C cells assessed as cell growth inhibition after 48 hrs by SRB assay	[PMID: 27097152]
SN12C	GI₅₀	0.813 μM Compound: 6, indotecan	Antiproliferative activity against human SN12C cells by SRB assay Antiproliferative activity against human SN12C cells by SRB assay	[PMID: 21710981]
UACC-62	GI₅₀	0.03 μM Compound: 6; MP400; NSC 724998	Antiproliferative activity in human UACC62 cells assessed as cell growth inhibition after 48 hrs by SRB assay Antiproliferative activity in human UACC62 cells assessed as cell growth inhibition after 48 hrs by SRB assay	[PMID: 27097152]
UACC-62	GI₅₀	0.03 μM Compound: 6, indotecan	Antiproliferative activity against human UACC62 cells by SRB assay Antiproliferative activity against human UACC62 cells by SRB assay	[PMID: 21710981]

In Vitro

Indotecan (48 h) inhibits the proliferation of L. infantum promastigotes, ex vivo-infected splenocytes, and uninfected splenocytes, with IC₅₀s of 0.10 μM, 0.10 μM, and 57.16 μM, respectively^[2].
Indotecan (1.1-90 μM; 30 min) induces TopI-DNA complexes and inhibits DNA synthesis in L. infantum cultures^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Indotecan (2.5 mg/kg; i.p. every 2 d for 15 d) depletes the parasitic burden in the spleen and liver of visceral leishmaniasis mice^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Female BALB/c mice (4-6 weeks) were infected with metacyclic parasites intravenously through the tail vein^[2]
Dosage:	2.5 mg/kg body weight per injection
Administration:	Intraperitoneally every 2 days for 15 days (total, eight doses)
Result:	A drastic reduction of the number of transforming amastigotes recovered from the target organs of drug-treated animals was observed.

Clinical Trial

Molecular Weight

478.49

Formula

C₂₆H₂₆N₂O₇

CAS No.

915303-09-2

Appearance

Solid

Color

White to gray

SMILES

O=C1C2=CC(OC)=C(OC)C=C2C3=C(C(C=C(OCO4)C4=C5)=C5C3=O)N1CCCN6CCOCC6

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, stored under nitrogen

*In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)

Solvent & Solubility

In Vitro:

DMSO : 1.23 mg/mL (2.57 mM; ultrasonic and adjust pH to 6 with 1 M HCL; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.0899 mL	10.4495 mL	20.8991 mL
5 mM	---	---	---
10 mM	---	---	---

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 0.12 mg/mL (0.25 mM); Clear solution

This protocol yields a clear solution of ≥ 0.12 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (1.2 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 0.12 mg/mL (0.25 mM); Clear solution

This protocol yields a clear solution of ≥ 0.12 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (1.2 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: ≥98.0%

Select Batch:

Data Sheet (279 KB) SDS (252 KB)

COA (257 KB) HNMR (264 KB)

Handling Instructions (2659 KB)

References

[1]. Balaña-Fouce R, et, al. Indotecan (LMP400) and AM13-55: two novel indenoisoquinolines show potential for treating visceral leishmaniasis. Antimicrob Agents Chemother. 2012 Oct;56(10):5264-70. [Content Brief]

[2]. Seol Y, et, al. Single-Molecule Supercoil Relaxation Assay as a Screening Tool to Determine the Mechanism and Efficacy of Human Topoisomerase IB Inhibitors. Mol Cancer Ther. 2015 Nov;14(11):2552-9. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO

1 mM

2.0899 mL

10.4495 mL

20.8991 mL

52.2477 mL

Indotecan Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Indotecan915303-09-2LMP-400 NSC-724998LMP400LMP 400NSC724998NSC 724998NSC-724998TopoisomeraseP388HCT116MCF-7upercoiled DNAvisceral leishmaniasisInhibitorinhibitorinhibit

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Indotecan (Synonyms: LMP-400; NSC-724998)

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Other Forms of Indotecan:

Indotecan Related Antibodies

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View All Topoisomerase Isoform Specific Products:

Biological Activity

Purity & Documentation

References

Customer Review

Indotecan Related Antibodies

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Complete Stock Solution Preparation Table

Indotecan Related Classifications

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