2. Metabolic Enzyme/Protease NF-κB
  3. Endogenous Metabolite NF-κB
  4. Nervonic acid

Nervonic acid  (Synonyms: Selacholeic acid; cis-15-Tetracosenoic acid)

Cat. No.: HY-N2526 Purity: 99.95%
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Nervonic acid is a monounsaturated fatty acid with oral activity. Nervonic acid exerts anti-inflammatory activity by inhibiting NF-κB signaling. Nervonic acid can be used in the study of neurodegenerative diseases.

For research use only. We do not sell to patients.

Nervonic acid Chemical Structure

Nervonic acid Chemical Structure

CAS No. : 506-37-6

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10 mM * 1 mL in DMSO
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Based on 3 publication(s) in Google Scholar

Other Forms of Nervonic acid:

Nervonic acid Related Antibodies

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NF-κB NF-κB1/p50 RelA/p65 RelB c-Rel

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

Nervonic acid is a monounsaturated fatty acid with oral activity. Nervonic acid exerts anti-inflammatory activity by inhibiting NF-κB signaling. Nervonic acid can be used in the study of neurodegenerative diseases[1][2][3].

IC50 & Target

Human Endogenous Metabolite

 

In Vitro

Nervonic acid (0.01 μM, 48 h) can improve the activity of PC-12 cells and play a role as a neuroprotective mediator in the brain[1].
Nervonic acid (12.5, 25, 50 μM, 24 h) can decrease the inflammatory response of RAW264.7 cells induced by LPS and inhibit the activation of key signal pathways related to inflammation[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Nervonic acid Related Antibodies

Cell Viability Assay[1]

Cell Line: PC-12
Concentration: 0.001, 0.01, 0.1, 1. 10 μM
Incubation Time: 48 h
Result: Increased cell viability pretreated with a very low concentration.

Western Blot Analysis[3]

Cell Line: RAW264.7
Concentration: 12.5, 25, 50 μM
Incubation Time: 24 h
Result: Decreased the expression of TLR4, JNK, P38 and NF-κB.
In Vivo

Nervonic acid (20, 40, 60 mg/kg, gavage for 7 days) protects the motor system against motor disorders in a PD mouse model[2].
Nervonic acid (5, 50, 100 mg/kg, dissolved in corn oil for 7 days) improves colonic inflammation by inhibiting the NF-κB signaling pathway in DSS-induced colitis mice[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: MPTP-induced PD mice model[2]
Dosage: 20, 40, 60 mg/kg
Administration: i.g. for 7 days
Result: Reversed behavioral deficits inhibited the α-synuclein expression and regulated oxidative stress response.
Animal Model: DSS-induced colitis mice model[3]
Dosage: 5, 50, 100 mg/kg
Administration: i.g. for 7 days
Result: Improved the symptoms of diarrhea, weight loss and fecal occidental blood.
Reduced the levels of COX-2 and iNOS.
Molecular Weight

366.62

Formula

C24H46O2
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

CCCCCCCC/C=C\CCCCCCCCCCCCCC(O)=O
Structure Classification
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (272.76 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.7276 mL 13.6381 mL 27.2762 mL
5 mM 0.5455 mL 2.7276 mL 5.4552 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 1.67 mg/mL (4.56 mM); Clear solution

    This protocol yields a clear solution of ≥ 1.67 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (16.7 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 1.67 mg/mL (4.56 mM); Clear solution

    This protocol yields a clear solution of ≥ 1.67 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (16.7 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation

Purity: 99.95%

SDS (394 KB)

COA (237 KB) HNMR (269 KB) RP-HPLC (241 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.7276 mL 13.6381 mL 27.2762 mL 68.1905 mL
5 mM 0.5455 mL 2.7276 mL 5.4552 mL 13.6381 mL
10 mM 0.2728 mL 1.3638 mL 2.7276 mL 6.8190 mL
15 mM 0.1818 mL 0.9092 mL 1.8184 mL 4.5460 mL
20 mM 0.1364 mL 0.6819 mL 1.3638 mL 3.4095 mL
25 mM 0.1091 mL 0.5455 mL 1.0910 mL 2.7276 mL
30 mM 0.0909 mL 0.4546 mL 0.9092 mL 2.2730 mL
40 mM 0.0682 mL 0.3410 mL 0.6819 mL 1.7048 mL
50 mM 0.0546 mL 0.2728 mL 0.5455 mL 1.3638 mL
60 mM 0.0455 mL 0.2273 mL 0.4546 mL 1.1365 mL
80 mM 0.0341 mL 0.1705 mL 0.3410 mL 0.8524 mL
100 mM 0.0273 mL 0.1364 mL 0.2728 mL 0.6819 mL
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Nervonic acid Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Nervonic acid506-37-6Selacholeic acid cis-15-Tetracosenoic acidEndogenous MetaboliteNF-κBNuclear factor-κBNuclear factor-kappaBAnti-inflammatoryPDPC-12RAW264.7DSS-induced colitisInhibitorinhibitorinhibit

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