Nutlin-3b

Name: Nutlin-3b
Brand: MedChemExpress
SKU: HY-15335
Rating: 4.5 (1 reviews)

Cat. No.: HY-15335 Purity: 98.78%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

Nutlin-3b, the inactive form of Nutlin-3 (HY-50696), is a p53/MDM2 inhibitor with an IC₅₀ of 13.6 μM. Nutlin-3b is 150 times less potent in binding to MDM2 than Nutlin-3a (HY-10029). Nutlin-3b is promising for research of cancers.

For research use only. We do not sell to patients.

Nutlin-3b Chemical Structure

CAS No. : 675576-97-3

Size	Price	Stock	Quantity

Free Sample (0.1 - 0.5 mg)		Apply Now
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 128	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 128	In-stock	Estimated Time of Arrival: December 31
Solid
5 mg	USD 100	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 170	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 540	In-stock	Estimated Time of Arrival: December 31
100 mg		Get quote
200 mg		Get quote

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Customer Review

Based on 1 publication(s) in Google Scholar

Other Forms of Nutlin-3b:

Nutlin-3a In-stock
Nutlin-3 In-stock

1 Publications Citing Use of MCE Nutlin-3b

•bioRxiv. 2024 Oct 9:2024.10.08.617155. [Abstract]

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Biological Activity
Purity & Documentation
References
Customer Review

Description

IC₅₀ & Target

IC50: 13.6 μM (MDM2/p53)^[1]

Cellular Effect

Cell Line	Type	Value	Description	References
A549	IC₅₀	10 μM Compound: Nutlin-3	Cytotoxicity against human A549 cells assessed as reduction in cell viability incubated for 24 hrs by CCK8 assay Cytotoxicity against human A549 cells assessed as reduction in cell viability incubated for 24 hrs by CCK8 assay	[PMID: 28797774]
A549	IC₅₀	2.4 μM Compound: Nutlin-3	Antiproliferative activity against human A549 cells measured after 24 hrs by CCK8 assay Antiproliferative activity against human A549 cells measured after 24 hrs by CCK8 assay	[PMID: 31047751]
HCT-116	GI₅₀	1.52 μM Compound: Nutlin-3	Antiproliferative activity against human HCT116 cells assessed as growth inhibition after 3 days by SRB assay Antiproliferative activity against human HCT116 cells assessed as growth inhibition after 3 days by SRB assay	[PMID: 28743509]
HCT-116	IC₅₀	28.4 μM Compound: Nutlin-3	Cytotoxicity against human HCT116 cells assessed as reduction in cell viability incubated for 24 hrs by CCK8 assay Cytotoxicity against human HCT116 cells assessed as reduction in cell viability incubated for 24 hrs by CCK8 assay	[PMID: 28797774]
HCT-116	IC₅₀	4 μM Compound: Nutlin-3	Antiproliferative activity against human HCT116 cells measured after 24 hrs by CCK8 assay Antiproliferative activity against human HCT116 cells measured after 24 hrs by CCK8 assay	[PMID: 31047751]
MCF7	IC₅₀	12.1 μM Compound: Nutlin-3	Antiproliferative activity against human MCF7 cells measured after 24 hrs by CCK8 assay Antiproliferative activity against human MCF7 cells measured after 24 hrs by CCK8 assay	[PMID: 31047751]
MCF7	IC₅₀	14.9 μM Compound: Nutlin-3	Cytotoxicity against human MCF7 cells assessed as reduction in cell viability incubated for 24 hrs by CCK8 assay Cytotoxicity against human MCF7 cells assessed as reduction in cell viability incubated for 24 hrs by CCK8 assay	[PMID: 28797774]
SAOS-2	IC₅₀	20.16 μM Compound: Nutlin-3	Cytotoxicity against p53 deficient human Saos2 cells assessed as cell growth inhibition after 24 hrs by MTT assay Cytotoxicity against p53 deficient human Saos2 cells assessed as cell growth inhibition after 24 hrs by MTT assay	[PMID: 28482147]
U2OS	IC₅₀	2.66 μM Compound: Nutlin-3	Cytotoxicity against wild type p53 expressing human U2OS cells assessed as cell growth inhibition after 24 hrs by MTT assay Cytotoxicity against wild type p53 expressing human U2OS cells assessed as cell growth inhibition after 24 hrs by MTT assay	[PMID: 28482147]
U-87MG ATCC	IC₅₀	5.93 μM Compound: Nutlin-3	Cytotoxicity against human U87 cells assessed as reduction in cell viability after 24 hrs by MTT assay Cytotoxicity against human U87 cells assessed as reduction in cell viability after 24 hrs by MTT assay	[PMID: 28916339]

In Vitro

Nutlin-3b (0-8 μM, 8 h) shows a potent antiproliferative activity in MDA-MB-435 and SW480 cells harboring mutant p53^[1].
Nutlin-3b (10 μM, 24 h and 48 h) has no influence on cell proliferation and apoptosis in wild-type p53 (HCT116)^[1].
Nutlin-3b (10 μM, 4, 24 and 48 h) slightly decreases p53 mRNA levels in sensitive liposarcoma cells^[2].
Nutlin-3b (20 μM, 60 min) interfere with P-gp-mediated drug efflux in UKF-NB-3rVCR10 neuroblastoma cells^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

581.49

Formula

C₃₀H₃₀Cl₂N₄O₄

CAS No.

675576-97-3

Appearance

Solid

Color

Off-white to yellow

SMILES

O=C1NCCN(C(N2[C@@H](C3=CC=C(Cl)C=C3)[C@@H](C4=CC=C(Cl)C=C4)N=C2C5=CC=C(OC)C=C5OC(C)C)=O)C1

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

Solvent & Solubility

In Vitro:

DMSO : ≥ 100 mg/mL (171.97 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.7197 mL	8.5986 mL	17.1972 mL
5 mM	0.3439 mL	1.7197 mL	3.4394 mL
10 mM	0.1720 mL	0.8599 mL	1.7197 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (4.30 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (4.30 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 98.78%

Select Batch:

Data Sheet (282 KB) SDS (762 KB)

COA (262 KB) LCMS (95 KB)

Handling Instructions (2659 KB)

References

[1]. Vassilev LT, et al. In vivo activation of the p53 pathway by small-molecule antagonists of MDM2. Science. 2004 Feb 6;303(5659):844-8. [Content Brief]

[2]. Müller CR, et al. Potential for treatment of liposarcomas with the MDM2 antagonist Nutlin-3A. Int J Cancer. 2007 Jul 1;121(1):199-205. [Content Brief]

[3]. Michaelis M, et al. Reversal of P-glycoprotein-mediated multidrug resistance by the murine double minute 2 antagonist nutlin-3. Cancer Res. 2009 Jan 15;69(2):416-21. [Content Brief]

[4]. Zhang F, et al. MDM2 antagonist nutlin-3a reverses mitoxantrone resistance by inhibiting breast cancer resistance protein mediated drug transport. Biochem Pharmacol. 2011 Jul 1;82(1):24-34. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	1.7197 mL	8.5986 mL	17.1972 mL	42.9930 mL
	5 mM	0.3439 mL	1.7197 mL	3.4394 mL	8.5986 mL
	10 mM	0.1720 mL	0.8599 mL	1.7197 mL	4.2993 mL
	15 mM	0.1146 mL	0.5732 mL	1.1465 mL	2.8662 mL
	20 mM	0.0860 mL	0.4299 mL	0.8599 mL	2.1497 mL
	25 mM	0.0688 mL	0.3439 mL	0.6879 mL	1.7197 mL
	30 mM	0.0573 mL	0.2866 mL	0.5732 mL	1.4331 mL
	40 mM	0.0430 mL	0.2150 mL	0.4299 mL	1.0748 mL
	50 mM	0.0344 mL	0.1720 mL	0.3439 mL	0.8599 mL
	60 mM	0.0287 mL	0.1433 mL	0.2866 mL	0.7166 mL
	80 mM	0.0215 mL	0.1075 mL	0.2150 mL	0.5374 mL
	100 mM	0.0172 mL	0.0860 mL	0.1720 mL	0.4299 mL

Nutlin-3b Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Nutlin-3b675576-97-3MDM-2/p53E1/E2/E3 EnzymeE1 activating enzymeE2 conjugating enzymeE3 ligating enzymeUbiquitin activating enzymeUbiquitin conjugating enzymeUbiquitin ligaseinactive formp53MDM2cancersInhibitorinhibitorinhibit

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Nutlin-3b

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Other Forms of Nutlin-3b:

Nutlin-3b Related Antibodies

1 Publications Citing Use of MCE Nutlin-3b

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•Related Small Molecules:

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Biological Activity

Purity & Documentation

References

Customer Review

Nutlin-3b Related Antibodies

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Complete Stock Solution Preparation Table

Nutlin-3b Related Classifications

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