Guanabenz hydrochloride

Name: Guanabenz hydrochloride
Brand: MedChemExpress
SKU: HY-12724A
Rating: 5.0 (2 reviews)

Cat. No.: HY-12724A Purity: 99.95%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

Guanabenz hydrochloride is an orally active α-2-adrenoceptor agonist. Guanabenz hydrochloride has antihypertensive effect and antiparasitic activity. Guanabenz hydrochloride interferes ER stress-signalling and has protective effects in cardiac myocytes. Guanabenz hydrochloride also is used for the research of high blood pressure.

For research use only. We do not sell to patients.

Guanabenz hydrochloride Chemical Structure

CAS No. : 23113-43-1

Size	Price	Stock	Quantity
25 mg	USD 43	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 80	In-stock	Estimated Time of Arrival: December 31
100 mg	USD 150	In-stock	Estimated Time of Arrival: December 31
200 mg		Get quote
500 mg		Get quote

or Bulk Inquiry

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 2 publication(s) in Google Scholar

Other Forms of Guanabenz hydrochloride:

Guanabenz Acetate In-stock
(E)-Guanabenz Get quote
Guanabenz Get quote

2 Publications Citing Use of MCE Guanabenz hydrochloride

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

View All Parasite Isoform Specific Products:

View All Isoforms

Amebae Coccidia Leishmania Mite Plasmodium Toxoplasma Trypanosoma Schistosome

View All Adrenergic Receptor Isoform Specific Products:

View All Isoforms

α adrenergic receptor β adrenergic receptor

Biological Activity
Purity & Documentation
References
Customer Review

Description

IC₅₀ & Target

Toxoplasma

In Vitro

Guanabenz hydrochloride (0.5-50 μM, 24 h) is treated with increasing concentrations for 24 hours not affect cell viability^[1].
Guanabenz hydrochloride (0.5-50 μM, 24 h) alone not affects the UPR targets, neither on mRNA or protein level nor the phosphorylation status of eIF2a. Guanabenz also not induces GADD34 or the constitutively active form CReP^[1].
Guanabenz hydrochloride (0.5-50 μM, 24 h) alone not induces ER stress in neonatal rat cardiomyocytes^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[1]

Cell Line:	Neonatal rat cardiac myocytes (NRCM)
Concentration:	0.5-50 μM
Incubation Time:	24 h
Result:	Did not affect cell survival.

Western Blot Analysis^[1]

Cell Line:	Neonatal rat cardiac myocytes (NRCM)
Concentration:	0.5-50 μM
Incubation Time:	24 h
Result:	Increased the levels of low panel concentration-dependent UPR targets proteins.

RT-PCR^[1]

Cell Line:	Neonatal rat cardiac myocytes (NRCM)
Concentration:	0.5-50 μM
Incubation Time:	24 h
Result:	Did not affect levels of UPR targets.

In Vivo

Guanabenz hydrochloride (5 mg/kg/day; i.p.; for 3 weeks) can reproducibly reduce brain cyst burden^[2].
Guanabenz hydrochloride (5 mg /kg/d, i.p., oral; 10 mg/kg/d, gavage; for 3 weeks) reverses Toxoplasma-induced hyperactivity in latently infected mice^[2].
Guanabenz hydrochloride (100 and 320 μg/kg and 1 mg/kg, i.v., over a period of 5 min at intervals of 40 min) reduces sympathetic outflow, heart rate and blood pressure in debuffered cats^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	BALB/cJ mice^[2]
Dosage:	5 mg/kg
Administration:	5 mg/kg/day; i.p. ; for 3 weeks
Result:	Reduced the latent brain cysts in both male and female BALB/cJ mice.

Animal Model:	BALB/cJ mice^[2]
Dosage:	5 mg/kg; 10 mg/kg
Administration:	5 mg /kg/d, i.p., oral; 10 mg/kg/d, gavage; for 3 weeks
Result:	Reversed parasite-induced hyperactivity to near-baseline levels.

Animal Model:	Cats^[3]
Dosage:	100 and 320 μg/kg and 1 mg/kg
Administration:	100 and 320 μg/kg and 1 mg/kg, i.v., over a period of 5 min at intervals of 40 min
Result:	Declined markedly blood pressure and nerve activity.

Clinical Trial

Molecular Weight

267.55

Formula

C₈H₉Cl₃N₄

CAS No.

23113-43-1

Appearance

Solid

Color

White to off-white

SMILES

ClC1=C(/C=N/NC(N)=N)C(Cl)=CC=C1.Cl

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (373.77 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	3.7377 mL	18.6883 mL	37.3766 mL
5 mM	0.7475 mL	3.7377 mL	7.4753 mL
10 mM	0.3738 mL	1.8688 mL	3.7377 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (9.34 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (9.34 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (9.34 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.95%

Select Batch:

Data Sheet (280 KB) SDS (252 KB)

COA (249 KB) HNMR (252 KB) LCMS (94 KB)

Handling Instructions (2659 KB)

References

[1]. Christiane Neuber, et al. Guanabenz interferes with ER stress and exerts protective effects in cardiac myocytes. PLoS One. 2014 Jun 3;9(6):e98893. [Content Brief]

[2]. Jennifer Martynowicz, et al. Guanabenz Reverses a Key Behavioral Change Caused by Latent Toxoplasmosis in Mice by Reducing Neuroinflammation. mBio. 2019 Apr 30;10(2):e00381-19. [Content Brief]

[3]. T Baum, et al. Studies on the centrally mediated hypotensive activity of guanabenz. Eur J Pharmacol. 1976 May;37(1):31-44. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	3.7377 mL	18.6883 mL	37.3766 mL	93.4415 mL
	5 mM	0.7475 mL	3.7377 mL	7.4753 mL	18.6883 mL
	10 mM	0.3738 mL	1.8688 mL	3.7377 mL	9.3442 mL
	15 mM	0.2492 mL	1.2459 mL	2.4918 mL	6.2294 mL
	20 mM	0.1869 mL	0.9344 mL	1.8688 mL	4.6721 mL
	25 mM	0.1495 mL	0.7475 mL	1.4951 mL	3.7377 mL
	30 mM	0.1246 mL	0.6229 mL	1.2459 mL	3.1147 mL
	40 mM	0.0934 mL	0.4672 mL	0.9344 mL	2.3360 mL
	50 mM	0.0748 mL	0.3738 mL	0.7475 mL	1.8688 mL
	60 mM	0.0623 mL	0.3115 mL	0.6229 mL	1.5574 mL
	80 mM	0.0467 mL	0.2336 mL	0.4672 mL	1.1680 mL
	100 mM	0.0374 mL	0.1869 mL	0.3738 mL	0.9344 mL