GW 610 (Synonyms: NSC 721648)

Name: GW 610
Brand: MedChemExpress
SKU: HY-118474
Rating: 4.5 (1 reviews)

Cat. No.: HY-118474 Purity: 99.78%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

GW 610 (NSC 721648), an antitumor agent, shows potent and selective anticancer activity against lung, colon, and breast cancer cell lines.

For research use only. We do not sell to patients.

GW 610 Chemical Structure

CAS No. : 872726-44-8

Size	Price	Stock	Quantity
5 mg	USD 80	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 130	In-stock	Estimated Time of Arrival: December 31
25 mg	USD 280	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 450	In-stock	Estimated Time of Arrival: December 31
100 mg	USD 720	In-stock	Estimated Time of Arrival: December 31
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500 mg		Get quote

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Based on 1 publication(s) in Google Scholar

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Biological Activity
Purity & Documentation
References
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Description

GW 610 (NSC 721648), an antitumor agent, shows potent and selective anticancer activity against lung, colon, and breast cancer cell lines^[1]^[2]^[3].

Cellular Effect

Cell Line	Type	Value	Description	References
A549	IC₅₀	15.88 μM Compound: 1, PMX 610	Cytotoxicity against human A549 cells after 24 hrs by MTT assay Cytotoxicity against human A549 cells after 24 hrs by MTT assay	[PMID: 22858144]
COLO 205	IC₅₀	13.94 μM Compound: 1, PMX 610	Cytotoxicity against human COLO205 cells after 24 hrs by MTT assay Cytotoxicity against human COLO205 cells after 24 hrs by MTT assay	[PMID: 22858144]
MCF-10A	IC₅₀	25.22 μM Compound: 1, PMX 610	Cytotoxicity against human MCF10A cells after 24 hrs by MTT assay Cytotoxicity against human MCF10A cells after 24 hrs by MTT assay	[PMID: 22858144]
MCF7	GI₅₀	< 0.0001 μM Compound: 5, PMX610, NSC-721648	Antiproliferative activity against estrogen receptor positive human MCF7 cells after 72 hrs by MTT assay Antiproliferative activity against estrogen receptor positive human MCF7 cells after 72 hrs by MTT assay	[PMID: 18666770]
MCF7	GI₅₀	0.514 μM Compound: 5, PMX610, NSC-721648	Antiproliferative activity against human estrogen receptor positive MCF7 cells after 72 hrs by MTT assay in presence of aryl hydrocarbon receptor antagonist alpha-naphthoflavone Antiproliferative activity against human estrogen receptor positive MCF7 cells after 72 hrs by MTT assay in presence of aryl hydrocarbon receptor antagonist alpha-naphthoflavone	[PMID: 18666770]
MCF7	IC₅₀	20.22 μM Compound: 1, PMX 610	Cytotoxicity against human MCF7 cells after 24 hrs by MTT assay Cytotoxicity against human MCF7 cells after 24 hrs by MTT assay	[PMID: 22858144]
MDA-MB-468	GI₅₀	< 0.0001 μM Compound: 5, PMX610, NSC-721648	Antiproliferative activity against estrogen receptor deficient human MDA468 cells after 72 hrs by MTT assay Antiproliferative activity against estrogen receptor deficient human MDA468 cells after 72 hrs by MTT assay	[PMID: 18666770]
MDA-MB-468	GI₅₀	0.27 μM Compound: 5, PMX610, NSC-721648	Antiproliferative activity against estrogen receptor deficient human MDA468 cells after 72 hrs by MTT assay in presence of aryl hydrocarbon receptor antagonist alpha-naphthoflavone Antiproliferative activity against estrogen receptor deficient human MDA468 cells after 72 hrs by MTT assay in presence of aryl hydrocarbon receptor antagonist alpha-naphthoflavone	[PMID: 18666770]

In Vitro

GW 610 inhibits the proliferation of MCF-7, MDA 468, KM 12, and HCC 2998 cells, with GI₅₀s of <0.1 nM, <0.1 nM, 290 nM, and 0.25 nM, respectively^[1].
GW 610 (0.1-1 μM) induces CYP1A1 mRNA and protein in MDA-MB-468, MCF-7, KM12, and HCC2998 cells, whereas CYP2S1 and CYP2W1 are induced only in breast cancer cells^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis^[3]

Cell Line:	MDA-MB-468, MCF-7, KM12, and HCC2998 cells
Concentration:	0.1, 1 μM
Incubation Time:	48 hours
Result:	Induced CYP1A1 mRNA and protein in all cell lines including KM12 and HCC2998 CRC cells. Induced CYP2S1 and CYP2W1 mRNA and protein expression in MDA-MB-468 and MCF-7 breast cancer cells.

Molecular Weight

289.32

Formula

C₁₅H₁₂FNO₂S

CAS No.

872726-44-8

Appearance

Solid

Color

Light yellow to brown

SMILES

FC1=CC=C(SC(C2=CC=C(OC)C(OC)=C2)=N3)C3=C1

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility

In Vitro:

DMSO : 25 mg/mL (86.41 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	3.4564 mL	17.2819 mL	34.5638 mL
5 mM	0.6913 mL	3.4564 mL	6.9128 mL
10 mM	0.3456 mL	1.7282 mL	3.4564 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (8.64 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.78%

Select Batch:

Data Sheet (273 KB) SDS (251 KB)

COA (246 KB) HNMR (247 KB) LCMS (265 KB)

Handling Instructions (2659 KB)

References

[1]. Mortimer CG, et, al. Antitumor benzothiazoles. 26.(1) 2-(3,4-dimethoxyphenyl)-5-fluorobenzothiazole (GW 610, NSC 721648), a simple fluorinated 2-arylbenzothiazole, shows potent and selective inhibitory activity against lung, colon, and breast cancer cell [Content Brief]

[2]. Aiello S, et, al. Synthesis and biological properties of benzothiazole, benzoxazole, and chromen-4-one analogues of the potent antitumor agent 2-(3,4-dimethoxyphenyl)-5-fluorobenzothiazole (PMX 610, NSC 721648). J Med Chem. 2008 Aug 28;51(16):5135-9. [Content Brief]

[3]. Tan BS, et, al. CYP2S1 and CYP2W1 mediate 2-(3,4-dimethoxyphenyl)-5-fluorobenzothiazole (GW-610, NSC 721648) sensitivity in breast and colorectal cancer cells. Mol Cancer Ther. 2011 Oct;10(10):1982-92. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	3.4564 mL	17.2819 mL	34.5638 mL	86.4095 mL
	5 mM	0.6913 mL	3.4564 mL	6.9128 mL	17.2819 mL
	10 mM	0.3456 mL	1.7282 mL	3.4564 mL	8.6410 mL
	15 mM	0.2304 mL	1.1521 mL	2.3043 mL	5.7606 mL
	20 mM	0.1728 mL	0.8641 mL	1.7282 mL	4.3205 mL
	25 mM	0.1383 mL	0.6913 mL	1.3826 mL	3.4564 mL
	30 mM	0.1152 mL	0.5761 mL	1.1521 mL	2.8803 mL
	40 mM	0.0864 mL	0.4320 mL	0.8641 mL	2.1602 mL
	50 mM	0.0691 mL	0.3456 mL	0.6913 mL	1.7282 mL
	60 mM	0.0576 mL	0.2880 mL	0.5761 mL	1.4402 mL
	80 mM	0.0432 mL	0.2160 mL	0.4320 mL	1.0801 mL

GW 610 Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

GW 610872726-44-8NSC 721648GW610GW-610NSC721648NSC-721648OthersantitumorlungcolonbreastInhibitorinhibitorinhibit

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GW 610 (Synonyms: NSC 721648)

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