2. MAPK/ERK Pathway
  3. JNK
  4. JNK-IN-7

JNK-IN-7  (Synonyms: JNK inhibitor)

Cat. No.: HY-15617 Purity: 98.92%
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JNK-IN-7 is a potent JNK inhibitor with IC50 of 1.5, 2 and 0.7 nM for JNK1, JNK2 and JNK3, respectively.

For research use only. We do not sell to patients.

JNK-IN-7 Chemical Structure

JNK-IN-7 Chemical Structure

CAS No. : 1408064-71-0

Size Price Stock Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 409 In-stock
Solution
10 mM * 1 mL in DMSO USD 409 In-stock
Solid
1 mg USD 179 In-stock
5 mg USD 377 In-stock
10 mg USD 487 In-stock
50 mg USD 1315 In-stock
100 mg USD 2097 In-stock
200 mg   Get quote  
500 mg   Get quote  

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Based on 4 publication(s) in Google Scholar

JNK-IN-7 Related Antibodies

Top Publications Citing Use of Products

View All JNK Isoform Specific Products:

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JNK1 JNK2 JNK3 JNK

  • Biological Activity

  • Protocol

  • Purity & Documentation

  • References

  • Customer Review

Description

JNK-IN-7 is a potent JNK inhibitor with IC50 of 1.5, 2 and 0.7 nM for JNK1, JNK2 and JNK3, respectively.

IC50 & Target[1]

JNK3

0.7 nM (IC50)

JNK1

1.5 nM (IC50)

JNK2

2 nM (IC50)

Cellular Effect
Cell Line Type Value Description References
A-375 IC50
0.244 μM
Compound: 71
Inhibition of JNK3-mediated c-jun phosphorylation in human A375 cells after 1 hr incubation
Inhibition of JNK3-mediated c-jun phosphorylation in human A375 cells after 1 hr incubation
[PMID: 25415535]
HeLa IC50
0.13 μM
Compound: 71
Inhibition of JNK3-mediated c-jun phosphorylation in human HeLa cells after 1 hr incubation
Inhibition of JNK3-mediated c-jun phosphorylation in human HeLa cells after 1 hr incubation
[PMID: 25415535]
In Vitro

JNK-IN-7 is a relatively selective JNK inhibitor in cells. In addition to JNK 1, 2, 3, JNK-IN-7 also binds to IRAK1(IC50=14.1 nM), YSK4 (IC50=4.8 nM), ERK3 (IC50=22 nM), PIK3C3, PIP5K3 and PIP4K2C[1]. Expression of divalent metal-ion transporter 1 (DMT1) in HCT116 is demonstrated to be markedly decreased under stimulation with TNF for 24 and 48 h, while JNK-IN-7 can significantly reverse the decrease. TNF can down-regulate DMT1 expression, while JNK-IN-7 can markedly suppress this function[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

JNK-IN-7 Related Antibodies
Molecular Weight

493.56

Formula

C28H27N7O2
CAS No.
Appearance

Solid

Color

White to gray

SMILES

O=C(C1=CC(NC(/C=C/CN(C)C)=O)=CC=C1)NC(C=C2)=CC=C2NC3=NC=CC(C4=CN=CC=C4)=N3
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 2 years
-20°C 1 year
Solvent & Solubility
In Vitro: 

DMSO : 33.33 mg/mL (67.53 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.0261 mL 10.1305 mL 20.2610 mL
5 mM 0.4052 mL 2.0261 mL 4.0522 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.75 mg/mL (5.57 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.75 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (27.5 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: 2.75 mg/mL (5.57 mM); Suspended solution; Need ultrasonic

    This protocol yields a suspended solution of 2.75 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (27.5 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
References
Cell Assay
[2]

Intestinal epithelial cell line (HCT116) is cultured in DMEM medium. To determine the mechanisms of TNF involved in regulating DMT1 expression, JNK-IN-7 (1 μM), NF-κB inhibitor (BAY 11-7082, 1 μM), and caspase-3/8 inhibitor (Z-DEVD-FMK, 50 μM) are also added into the culture medium. After 48 h of culture, cells are then collected to detect the expression of DMT1 by qRT-PCR[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.0261 mL 10.1305 mL 20.2610 mL 50.6524 mL
5 mM 0.4052 mL 2.0261 mL 4.0522 mL 10.1305 mL
10 mM 0.2026 mL 1.0130 mL 2.0261 mL 5.0652 mL
15 mM 0.1351 mL 0.6754 mL 1.3507 mL 3.3768 mL
20 mM 0.1013 mL 0.5065 mL 1.0130 mL 2.5326 mL
25 mM 0.0810 mL 0.4052 mL 0.8104 mL 2.0261 mL
30 mM 0.0675 mL 0.3377 mL 0.6754 mL 1.6884 mL
40 mM 0.0507 mL 0.2533 mL 0.5065 mL 1.2663 mL
50 mM 0.0405 mL 0.2026 mL 0.4052 mL 1.0130 mL
60 mM 0.0338 mL 0.1688 mL 0.3377 mL 0.8442 mL
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JNK-IN-7 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

JNK-IN-71408064-71-0JNK inhibitorJNKc-Jun N-terminal kinaseInhibitorinhibitorinhibit

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