2. Metabolic Enzyme/Protease Cell Cycle/DNA Damage NF-κB Immunology/Inflammation Apoptosis Anti-infection
  3. Endogenous Metabolite CDK Reactive Oxygen Species Apoptosis Enterovirus Bacterial
  4. Lacto-N-fucopentaose I

Lacto-N-fucopentaose I (LNFPI) is a human milk oligosaccharide (HMO), possessing antiviral and antibacterial activity. Lacto-N-fucopentaose I can reduce capsid protein VP1 to block virus adsorption, promote CDK2 and reduce cyclin E to recover cell cycle S phase block. Lacto-N-fucopentaose I inhibits ROS production and apoptosis in virus-infected cells. Lacto-N-fucopentaose I can also regulate intestinal microbiota to affect immune system development.

For research use only. We do not sell to patients.

Lacto-N-fucopentaose I Chemical Structure

Lacto-N-fucopentaose I Chemical Structure

CAS No. : 7578-25-8

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Based on 1 publication(s) in Google Scholar

Lacto-N-fucopentaose I Related Antibodies

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Description

Lacto-N-fucopentaose I (LNFPI) is a human milk oligosaccharide (HMO), possessing antiviral and antibacterial activity. Lacto-N-fucopentaose I can reduce capsid protein VP1 to block virus adsorption, promote CDK2 and reduce cyclin E to recover cell cycle S phase block. Lacto-N-fucopentaose I inhibits ROS production and apoptosis in virus-infected cells. Lacto-N-fucopentaose I can also regulate intestinal microbiota to affect immune system development[1].

IC50 & Target

Human Endogenous Metabolite

 

CDK2/cyclinE

 

In Vitro

Lacto-N-fucopentaose I (25-3200 μg/mL;48 h) exhibits certain cytotoxicity at 3200 μg/mL but no toxic reaction below 1600 μg/mL[1].
Lacto-N-fucopentaose I (25-1600 μg/mL; 14-18 h) can protect EV71-infected RD cells from death[1].
Lacto-N-fucopentaose I (100-400 μg/mL; 16 h) decreases markedly mRNA levels of VP1 and ROS production in EV71-infected RD cells at 400 μg/mL; leads to the recovery of EV71-induced S phase arrest in RD cells[1].
Lacto-N-fucopentaose I (100 and 200 μg/mL; 3 days) inhibits cell apoptosis in Caenorhabditis elegans; significantly decreases the levels of Egl-1, Ced-3 and Ced-4[1].
Lacto-N-fucopentaose I can reduce the abundance of Sphingobacterium, Stenotrophomonas and Achromobacter; can increase the abundance of Micromonospora, Vibrio, Acidibacter, Gaiella, Devosia, Steroidobacter, Variibacter, Dactylosporangium, RB41, Pir4_lineage, Pirellula, Haliangium, Roseiflexus, Pedomicrobium, and Bradyrhizobium.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Lacto-N-fucopentaose I Related Antibodies

Cell Viability Assay[1]

Cell Line: RD cells
Concentration: 25, 50, 100, 200, 400, 800, 1600 and 3200 μg/mL
Incubation Time: 48 h
Result: Exhibited certain cytotoxicity at 3200 μg/mL but no toxic reaction below 1600 μg/mL.

RT-PCR[1]

Cell Line: RD cells (infected with EV71)
Concentration: 100, 200 and 400 μg/mL
Incubation Time: 16 h
Result: Decreased markedly mRNA levels of VP1 only at 400 μg/mL.

Apoptosis Analysis[1]

Cell Line: RD cells (infected with EV71)
Concentration: 100, 200 and 400 μg/mL
Incubation Time: 16 h
Result: Decreased the rate of cells in early apoptosis to 10.9% ± 1.26% at 400 μg/mL, while the untreated EV71 group was 27.7% ± 2.13%.
Significantly inhibited the activity of caspase-3, caspase-8 and caspase-9.
Recovered the decreased mRNA expression of PAPR, NF-κB and Bcl-2 and properly regulated Bad and Fas into their normal levels.

Cell Cycle Analysis[1]

Cell Line: RD cells (infected with EV71)
Concentration: 100, 200 and 400 μg/mL
Incubation Time: 12 h
Result: Rescued EV71-induced S phase arrest, which promoted the transition of the G1 phase to the S phase.
Molecular Weight

853.77

Formula

C32H55NO25
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

CC(N[C@@H]1[C@H]([C@@H]([C@@H](CO)O[C@H]1O[C@]2([H])[C@H]([C@@H](O[C@H](CO)[C@@H]2O)O[C@@]([C@H](O)CO)([H])[C@H](O)[C@@H](O)C=O)O)O)O[C@@]3([H])[C@@H]([C@H]([C@@H](O)[C@@H](CO)O3)O)O[C@@]4([H])[C@H]([C@@H]([C@H](O)[C@H](C)O4)O)O)=O
Structure Classification
Initial Source

human milk
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility
In Vitro: 

H2O : 125 mg/mL (146.41 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.1713 mL 5.8564 mL 11.7128 mL
5 mM 0.2343 mL 1.1713 mL 2.3426 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

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V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
Purity & Documentation

Purity: ≥90.0%

COA (262 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
H2O 1 mM 1.1713 mL 5.8564 mL 11.7128 mL 29.2819 mL
5 mM 0.2343 mL 1.1713 mL 2.3426 mL 5.8564 mL
10 mM 0.1171 mL 0.5856 mL 1.1713 mL 2.9282 mL
15 mM 0.0781 mL 0.3904 mL 0.7809 mL 1.9521 mL
20 mM 0.0586 mL 0.2928 mL 0.5856 mL 1.4641 mL
25 mM 0.0469 mL 0.2343 mL 0.4685 mL 1.1713 mL
30 mM 0.0390 mL 0.1952 mL 0.3904 mL 0.9761 mL
40 mM 0.0293 mL 0.1464 mL 0.2928 mL 0.7320 mL
50 mM 0.0234 mL 0.1171 mL 0.2343 mL 0.5856 mL
60 mM 0.0195 mL 0.0976 mL 0.1952 mL 0.4880 mL
80 mM 0.0146 mL 0.0732 mL 0.1464 mL 0.3660 mL
100 mM 0.0117 mL 0.0586 mL 0.1171 mL 0.2928 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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Lacto-N-fucopentaose I Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Lacto-N-fucopentaose I7578-25-8LNFP IEndogenous MetaboliteCDKReactive Oxygen SpeciesApoptosisEnterovirusBacterialCyclin dependent kinaseRhinovirusHRVHRVsHEVHEVsHMOVP1intestinal microbiotaRD cellsanti-inflammatoryInhibitorinhibitorinhibit

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