2. Cell Cycle/DNA Damage Vitamin D Related/Nuclear Receptor Metabolic Enzyme/Protease Apoptosis NF-κB Immunology/Inflammation
  3. PPAR Apoptosis Reactive Oxygen Species
  4. Soyasaponin Ab

Soyasaponin Ab is an orally active soyasaponin. Soyasaponin Ab inhibits PPARγ transcriptional activity. Soyasaponin Ab induces apoptosis in high concentrations. Soyasaponin Ab exerts anti-obesity, anti-oxidation, anti-inflammation, anti-aging effects. Soyasaponin Ab prevents Scopolamine (HY-N0296)-induced memory impairment.

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Soyasaponin Ab Chemical Structure

Soyasaponin Ab Chemical Structure

CAS No. : 118194-13-1

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Soyasaponin Ab Related Antibodies

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1 Publications Citing Use of MCE Soyasaponin Ab

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Description

Soyasaponin Ab is an orally active soyasaponin. Soyasaponin Ab inhibits PPARγ transcriptional activity. Soyasaponin Ab induces apoptosis in high concentrations. Soyasaponin Ab exerts anti-obesity, anti-oxidation, anti-inflammation, anti-aging effects. Soyasaponin Ab prevents Scopolamine (HY-N0296)-induced memory impairment[1][2][3][4].

In Vitro

Soyasaponin Ab (25-100 μM, 8 days ) decreases triglyceride accumulation in a dose-dependent manner in 3T3-L1 adipocytes[1].
Soyasaponin Ab (25-100 μM, 24 h) suppresses the transcriptional activity of peroxisome proliferator-activated receptor γ (PPARγ) in HEK 293T cells[1].
Soyasaponin Ab (50-100 μM, 8 days ) markedly inhibits adipocyte differentiation and expression of various adipogenic marker genes (including adiponectin, ADD1/SREBP1c, aP2, Fas, and resistin) through the downregulation of the adipogenesis-related transcription factors PPARγ and C/EBPα in 3T3-L1 adipocytes[1].
Soyasaponin Ab (1-100 μM, 1 h) shows concentration dependent inhibition of lipid peroxidation in liposomes (IC50 = 14.5 μM)[2].
Soyasaponin Ab (10-50 μM, 48 h) regulates translocation of Nrf2 and protein expressions of the phase II antioxidant enzyme HO-1 and NQO1, through the ERK1/2 signaling pathway in HepG2 cells[2].
Soyasaponin Ab (1-400 μM, 48 h or 10 days) shows little toxicity below 50 μM and reduces the formation of colonies at the dose that above 50 μM and induces apoptosis in HepG2 cells[2].
Soyasaponin Ab (1-10 μM, 16 h or 4 h) significantly reduces Lipopolysaccharides (LPS) (HY-D1056)-stimulated IL-1β, TNF-α and TLR4 expression and PGE2 and NO production in peritoneal macrophages[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Soyasaponin Ab Related Antibodies

Cell Proliferation Assay[2]

Cell Line: HepG2 cells
Concentration: 50, 100, 200, 400 μM
Incubation Time: 10 days
Result: Reduced the formation of colonies in dose-dependent manner, especially in 200 and 400 μM treatment groups.

Cell Viability Assay[2]

Cell Line: HepG2 cells
Concentration: 1, 5, 10, 20, 50, 100, 200, 400 μM
Incubation Time: 48 h
Result: The cytotoxicity represented a slight increase after treated with higher concentrations after 48 h, especially for the cells treated over 200 μM. There were some morphological changes including loss of cellular geometry and reduction in proliferation of cells after a 100 μM or more treatment. No obvious change on the cell morphology of the groups treated less than 50 μM.

Apoptosis Analysis[2]

Cell Line: HepG2 cells
Concentration: 50, 100, 200, 400 μM
Incubation Time: 48 h
Result: Induced some extent of apoptosis in cells in a dose-dependent manner.

RT-PCR[1]

Cell Line: 3T3-L1
Concentration: 50, 100 μM
Incubation Time: 8 days
Result: Effectively suppressed PPARγ and CEBPα mRNA expression. Markedly reduced expression of various adipogenic marker genes, including adiponectin, ADD1/SREBP1c, aP2, Fas, and resistin.

Western Blot Analysis[1]

Cell Line: 3T3-L1
Concentration: 50, 100 μM
Incubation Time: 8 days
Result: Significantly suppressed the expression of PPARγ and CEBPα proteins at 50 and 100 μM.
In Vivo

Soyasaponin Ab (10-20 mg/kg; p.o.; daily for 5 days) shows inhibitory effect in 2,4,6-trinitrobenzene sulfonic acid (TNBS)-induced colitis in male ICR mice[3].
Soyasaponin Ab (5-40 mg/kg; p.o.; one hour before the trial) significantly prevents Scopolamine (HY-N0296)-induced memory impairment in male ICR mice[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Male ICR mice (18-22 g, 5 weeks)[4]
Dosage: 5, 10, 20, 40 mg/kg
Administration: Oral gavage (p.o.); One hour before the trial, and memory impairment was induced by intraperitoneal injection of Scopolamine (0.9 mg/kg) 30 min after oral administrations of test agents.
Result: Restored memory impairment to 86% at a dose of 10 mg/kg of untreated normal control mice in the passive avoidance task. Restored spontaneous alteration, which was lowered by Scopolamine on the Y-maze task. Significantly shortened the escape latencies on the fifth day.
Animal Model: Male institute of cancer research (ICR) mice (20-22 g, 4 weeks)[3]
Dosage: 10, 20 mg/kg
Administration: Oral gavage (p.o.); daily for 5 days
Result: Inhibited TNBS-induced body weight reduction, colon shortening, macroscopic score, and myeloperoxidase activity. Inhibited the expression of pro-inflammatory cytokines (TNF-α, IL-1β, IL-6). Ameliorated changes of TNBS-treated mouse colons which showed increased neutrophils, massive bowel edema, dense infiltration of the superficial layers of the mucosa, and epithelial cell disruption by large ulcerations. Inhibited the expression of TLR4, COX-2, and iNOS and the phosphorylation of IRAK1, IKK-β and p65, although it reversed IRAK-1 expression.
Molecular Weight

1437.52

Formula

C67H104O33
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

C[C@]12[C@]3(C([C@@]4([H])[C@](C)([C@@H]([C@H](O)C(C)(C)C4)O[C@@]5([H])[C@@H]([C@H]([C@@H](O)CO5)O[C@@]6([H])[C@@H]([C@H]([C@H](OC(C)=O)[C@@H](COC(C)=O)O6)OC(C)=O)OC(C)=O)O)CC3)=CC[C@]1([H])[C@@]7([C@@]([C@](C)([C@@H](O[C@@]8([H])[C@@H]([C@H]([C@H](O)[C@@H](C(O)=O)O8)O)O[C@@]9([H])[C@@H]([C@H]([C@@H](O)[C@@H](CO)O9)O)O[C@]%10([H])O[C@@H]([C@@H](O)[C@H](O)[C@H]%10O)CO)CC7)CO)([H])CC2)C)C
Structure Classification
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (69.56 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 0.6956 mL 3.4782 mL 6.9564 mL
5 mM 0.1391 mL 0.6956 mL 1.3913 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

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In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.5 mg/mL (1.74 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.5 mg/mL (1.74 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

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Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
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%
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Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation

Purity: 99.20%

SDS (252 KB)

COA (271 KB) HNMR (221 KB) RP-HPLC (279 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 0.6956 mL 3.4782 mL 6.9564 mL 17.3911 mL
5 mM 0.1391 mL 0.6956 mL 1.3913 mL 3.4782 mL
10 mM 0.0696 mL 0.3478 mL 0.6956 mL 1.7391 mL
15 mM 0.0464 mL 0.2319 mL 0.4638 mL 1.1594 mL
20 mM 0.0348 mL 0.1739 mL 0.3478 mL 0.8696 mL
25 mM 0.0278 mL 0.1391 mL 0.2783 mL 0.6956 mL
30 mM 0.0232 mL 0.1159 mL 0.2319 mL 0.5797 mL
40 mM 0.0174 mL 0.0870 mL 0.1739 mL 0.4348 mL
50 mM 0.0139 mL 0.0696 mL 0.1391 mL 0.3478 mL
60 mM 0.0116 mL 0.0580 mL 0.1159 mL 0.2899 mL
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Soyasaponin Ab Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Soyasaponin Ab118194-13-1PPARApoptosisReactive Oxygen SpeciesPeroxisome proliferator-activated receptorsadipogenesisadipogenic marker genes3T3-L1HepG2memory impairmentinflammatoryInhibitorinhibitorinhibit

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