Spiraeoside (Synonyms: Quercetin 4′-O-glucoside)

Name: Spiraeoside
Brand: MedChemExpress
SKU: HY-N8253
Rating: 4.5 (1 reviews)

Cat. No.: HY-N8253 Purity: 99.46%: FAQs
Data Sheet SDS COA Handling Instructions Technical Support

Spiraeoside, an orally active natural compound, exerts antioxidant activity, inhibits reactive oxygen species (ROS) and malondialdehyde production. Spiraeoside possesses antiallergic, anti-inflammatory and antitumor activities.

For research use only. We do not sell to patients.

Spiraeoside Chemical Structure

CAS No. : 20229-56-5

Size	Stock	Quantity
5 mg	In-stock	Estimated Time of Arrival: December 31
10 mg	Get quote
50 mg	Get quote

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Based on 1 publication(s) in Google Scholar

Other Forms of Spiraeoside:

Spiraeoside (Standard) Get quote

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Biological Activity
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References
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Description

Cellular Effect

Cell Line	Type	Value	Description	References
B16-4A5	IC₅₀	> 100 μM Compound: 8	Inhibition of melanogenesis in theophylline-stimulated mouse B16-4A5 cells after 72 hrs by microplate reader analysis Inhibition of melanogenesis in theophylline-stimulated mouse B16-4A5 cells after 72 hrs by microplate reader analysis	[PMID: 25987378]

In Vitro

Spiraeoside elevates HG stimulation-caused the decrease in the expression levels of p-Akt, nuclear Nrf2, and HO-1 in AC16 cells (the effects of Spiraeoside are reversed by LY294002)^[1].
Spiraeoside protects AC16 cells against HG-induced oxidative stress, cell injury, and apoptosis^[1].
Spiraeoside activates the PI3K/Akt/Nrf2 pathway in AC16 cells on exposure to HG^[1].
Spiraeoside protects AC16 cells against HG-induced apoptosis through the PI3K/Akt/Nrf2 pathway^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[1]

Cell Line:	AC16 cells.
Concentration:	1, 5, 10, or 20 μM.
Incubation Time:	0, 24, or 48 hours.
Result:	Inhibited AC16 cells viability (20 μM).

In Vivo

Spiraeoside (50 mg/kg, p.o.) shows ulcer preventive ability^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Male Wistar rats (6-8 weeks old)^[2].
Dosage:	50 mg/kg.
Administration:	Oral gavage an hour before inducing the lesions.
Result:	Decreased severity of the formed lesions.

Molecular Weight

464.38

Formula

C₂₁H₂₀O₁₂

CAS No.

20229-56-5

Appearance

Solid

Color

Light yellow to yellow

SMILES

O=C1C(C(OC(C(C=C2)=CC(O)=C2O[C@@H]([C@@H]([C@H]3O)O)O[C@@H]([C@H]3O)CO)=C1O)=CC(O)=C4)=C4O

Structure Classification

Initial Source

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvent & Solubility

In Vitro:

Ethanol : 10 mg/mL (21.53 mM; Need ultrasonic)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.1534 mL	10.7670 mL	21.5341 mL
5 mM	0.4307 mL	2.1534 mL	4.3068 mL
10 mM	0.2153 mL	1.0767 mL	2.1534 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% EtOH 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 1 mg/mL (2.15 mM); Clear solution

This protocol yields a clear solution of ≥ 1 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL EtOH stock solution (10.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% EtOH 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 1 mg/mL (2.15 mM); Clear solution

This protocol yields a clear solution of ≥ 1 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL EtOH stock solution (10.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% EtOH 90% Corn Oil
Solubility: ≥ 1 mg/mL (2.15 mM); Clear solution

This protocol yields a clear solution of ≥ 1 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL EtOH stock solution (10.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

Purity & Documentation

Purity: 99.46%

Select Batch:

Data Sheet (276 KB) SDS (393 KB)

COA (250 KB) HNMR (220 KB) CNMR (220 KB) RP-HPLC (151 KB)

Handling Instructions (2659 KB)

References

[1]. Hongyang Liu, et al. Spiraeoside protects human cardiomyocytes against high glucose-induced injury, oxidative stress, and apoptosis by activation of PI3K/Akt/Nrf2 pathway. J Biochem Mol Toxicol. 2020 Oct;34(10):e22548. [Content Brief]

[2]. Stevan Samardžić, et al. Antioxidant, anti-inflammatory and gastroprotective activity of Filipendula ulmaria (L.) Maxim. and Filipendula vulgaris Moench. J Ethnopharmacol. 2018 Mar 1;213:132-137. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

Ethanol	1 mM	2.1534 mL	10.7670 mL	21.5341 mL	53.8352 mL
	5 mM	0.4307 mL	2.1534 mL	4.3068 mL	10.7670 mL
	10 mM	0.2153 mL	1.0767 mL	2.1534 mL	5.3835 mL
	15 mM	0.1436 mL	0.7178 mL	1.4356 mL	3.5890 mL
	20 mM	0.1077 mL	0.5384 mL	1.0767 mL	2.6918 mL

Spiraeoside Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Spiraeoside20229-56-5Quercetin 4′-O-glucosideReactive Oxygen SpeciesPI3KAktNrf2ROSAC16Inhibitorinhibitorinhibit

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Spiraeoside (Synonyms: Quercetin 4′-O-glucoside)

Customer Review

Other Forms of Spiraeoside:

Spiraeoside Related Antibodies

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

Biological Activity

Purity & Documentation

References

Customer Review

Spiraeoside Related Antibodies

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

Spiraeoside Related Classifications

Keywords:

Your Recently Viewed Products:

Customer Review

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SAFETY DATA SHEET (SDS)

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