2. Neuronal Signaling Autophagy Metabolic Enzyme/Protease
  3. PINK1/Parkin Mitochondrial Metabolism
  4. T0467

T0467 activates parkin mitochondrial translocation in a PINK1-dependent manner in vitro. T0467 do not induce mitochondrial accumulation of PINK1in dopaminergic neurons. T0467 is a potential compound for PINK1-Parkin signaling activation, and can be used for parkinson's disease and related disorders research.

For research use only. We do not sell to patients.

T0467 Chemical Structure

T0467 Chemical Structure

CAS No. : 859518-94-8

Size Price Stock Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 363 In-stock
Solution
10 mM * 1 mL in DMSO USD 363 In-stock
Solid
5 mg USD 330 In-stock
10 mg USD 520 In-stock
50 mg USD 1850 In-stock
100 mg USD 2750 In-stock
200 mg   Get quote  
500 mg   Get quote  

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Customer Review

Based on 1 publication(s) in Google Scholar

T0467 Related Antibodies

Top Publications Citing Use of Products

1 Publications Citing Use of MCE T0467

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

T0467 activates parkin mitochondrial translocation in a PINK1-dependent manner in vitro. T0467 do not induce mitochondrial accumulation of PINK1in dopaminergic neurons. T0467 is a potential compound for PINK1-Parkin signaling activation, and can be used for parkinson's disease and related disorders research[1].

In Vitro

T0467 (2.5-20 μM; 3 hours) stimulates the mitochondrial translocation of GFP-Parkinover 12 μM in HeLa/GFP-Parkin cells[1]. When HeLa/GFP-Parkin cells are treated with 20 μM T0467 for 3 h, GTP-Parkin is translocated to the mitochondria in approximately 21% of cells[1].
T0467 does not show obvious toxicity in Drosophila at concentrations <50 μM. All cpds examined mitigated the PINK1 inactivation-mediated larval locomotion defects and mitochondrial morphological defects and reduced ATP production. T0467 and KTP improved the mitochondrial Ca2+ response in Drosophila larval muscles[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

T0467 Related Antibodies
Molecular Weight

441.49

Formula

C24H26F3N5
CAS No.
Appearance

Solid

Color

Off-white to light brown

SMILES

FC1=CC=C(CN2[C@H](CNCC2)C)C=C1C3=NC(NCCC4=CC(F)=CC(F)=C4)=NC=C3
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 33.33 mg/mL (75.49 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.2651 mL 11.3253 mL 22.6506 mL
5 mM 0.4530 mL 2.2651 mL 4.5301 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

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In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.5 mg/mL (5.66 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

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mg/kg

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g

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(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.2651 mL 11.3253 mL 22.6506 mL 56.6264 mL
5 mM 0.4530 mL 2.2651 mL 4.5301 mL 11.3253 mL
10 mM 0.2265 mL 1.1325 mL 2.2651 mL 5.6626 mL
15 mM 0.1510 mL 0.7550 mL 1.5100 mL 3.7751 mL
20 mM 0.1133 mL 0.5663 mL 1.1325 mL 2.8313 mL
25 mM 0.0906 mL 0.4530 mL 0.9060 mL 2.2651 mL
30 mM 0.0755 mL 0.3775 mL 0.7550 mL 1.8875 mL
40 mM 0.0566 mL 0.2831 mL 0.5663 mL 1.4157 mL
50 mM 0.0453 mL 0.2265 mL 0.4530 mL 1.1325 mL
60 mM 0.0378 mL 0.1888 mL 0.3775 mL 0.9438 mL
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T0467 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

T0467859518-94-8T 0467T-0467PINK1/ParkinMitochondrial Metabolismmitochondrial translocationparkindopaminergic neuronParkinson's diseasedisordersInhibitorinhibitorinhibit

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