2. MAPK/ERK Pathway Stem Cell/Wnt
  3. ERK
  4. Tenuifoliside A

Tenuifoliside A is isolated from Polygala tenuifolia, has anti-apoptotic and antidepressant-like effects. Tenuifoliside A exhibits its neneurotrophic effects and promotes cell proliferation through the ERK/CREB/BDNF signal pathway in C6 cells.

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Tenuifoliside A Chemical Structure

Tenuifoliside A Chemical Structure

CAS No. : 139726-35-5

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Based on 1 publication(s) in Google Scholar

Tenuifoliside A Related Antibodies

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Description

Tenuifoliside A is isolated from Polygala tenuifolia, has anti-apoptotic and antidepressant-like effects. Tenuifoliside A exhibits its neneurotrophic effects and promotes cell proliferation through the ERK/CREB/BDNF signal pathway in C6 cells[1].

In Vitro

Tenuifoliside A (6-30 μM; 24 hours) increases the C6 cells viability significantly by concentration dependent manner. But the viability of the 60 μM treatment group is suppressed. Only 60 μM TFSA induced the release of LDH, shows a toxic effect[1].
Tenuifoliside A (10 μM; 2, 5, 7, 10, 15, 30, 45 and 60 mins) elicits a rapid (starting at 2 min) and marked induction of ERK1/2 phosphorylation peaking at 5 min. And,the enhanced levels of phospho-ERK induced by TFSA are partially blocked by ERK inhibitor U0126[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Tenuifoliside A Related Antibodies
Molecular Weight

682.62

Formula

C31H38O17
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

OC[C@@]1([C@H]([C@H](O)[C@@H](CO)O1)OC(/C=C/C2=CC(OC)=C(OC)C(OC)=C2)=O)O[C@@H]3O[C@@H]([C@@H](O)[C@H](O)[C@H]3O)COC(C4=CC=C(O)C=C4)=O
Structure Classification
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

-20°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (146.49 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.4649 mL 7.3247 mL 14.6494 mL
5 mM 0.2930 mL 1.4649 mL 2.9299 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

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In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.5 mg/mL (3.66 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% Corn Oil

    Solubility: ≥ 2.5 mg/mL (3.66 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation

Purity: 98.07%

COA (255 KB) HNMR (272 KB) RP-HPLC (308 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 1.4649 mL 7.3247 mL 14.6494 mL 36.6236 mL
5 mM 0.2930 mL 1.4649 mL 2.9299 mL 7.3247 mL
10 mM 0.1465 mL 0.7325 mL 1.4649 mL 3.6624 mL
15 mM 0.0977 mL 0.4883 mL 0.9766 mL 2.4416 mL
20 mM 0.0732 mL 0.3662 mL 0.7325 mL 1.8312 mL
25 mM 0.0586 mL 0.2930 mL 0.5860 mL 1.4649 mL
30 mM 0.0488 mL 0.2442 mL 0.4883 mL 1.2208 mL
40 mM 0.0366 mL 0.1831 mL 0.3662 mL 0.9156 mL
50 mM 0.0293 mL 0.1465 mL 0.2930 mL 0.7325 mL
60 mM 0.0244 mL 0.1221 mL 0.2442 mL 0.6104 mL
80 mM 0.0183 mL 0.0916 mL 0.1831 mL 0.4578 mL
100 mM 0.0146 mL 0.0732 mL 0.1465 mL 0.3662 mL
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Tenuifoliside A Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Tenuifoliside A139726-35-5ERKExtracellular signal regulated kinasesC6cellsanti-apoptoticantidepressant-like,neneurotrophicInhibitorinhibitorinhibit

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