β-Glucogallin (Synonyms: 1-O-Galloyl-β-D-glucose)

Name: β-Glucogallin
Brand: MedChemExpress
SKU: HY-133708
Rating: 4.5 (1 reviews)

Cat. No.: HY-133708 Purity: 99.82%: FAQs
Data Sheet
SDS

COA
Handling Instructions Technical Support

β-Glucogallin is an orally active and selective aldose reductase (AKR1B1) inhibitor with an IC₅₀ value of 58 μM when using Glyceraldehyde (HY-128748) as AKR1B1 substrate. β-Glucogallin reduces ROS, PDGF, RAGE, and NF-κB. β-Glucogallin increases SOD. β-Glucogallin has antioxidant and hepatoprotective effects. β-Glucogallin can be used in retinal research.

For research use only. We do not sell to patients.

β-Glucogallin Chemical Structure

CAS No. : 13405-60-2

Size	Stock	Quantity
1 mg	In-stock	Estimated Time of Arrival: December 31
5 mg	In-stock	Estimated Time of Arrival: December 31
10 mg	In-stock	Estimated Time of Arrival: December 31
25 mg	In-stock	Estimated Time of Arrival: December 31
50 mg	Get quote
100 mg	Get quote

or Bulk Inquiry

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 1 publication(s) in Google Scholar

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

View All NF-κB Isoform Specific Products:

View All Isoforms

NF-κB NF-κB1/p50 RelA/p65 RelB c-Rel

View All PDGFR Isoform Specific Products:

View All Isoforms

PDGFR PDGFRβ PDGFRα

Biological Activity
Purity & Documentation
References
Customer Review

Description

IC₅₀ & Target

IC50: 58 μM (AKR1B1) when using Glyceraldehyde (HY-128748) as AKR1B1 substrate

Cellular Effect

Cell Line	Type	Value	Description	References
A549	ED₅₀	> 10 μg/mL Compound: 4	Cytotoxicity against human A549 cells by tetrazolium salt-based colorimetric assay Cytotoxicity against human A549 cells by tetrazolium salt-based colorimetric assay	[PMID: 1431932]
HCT-8	ED₅₀	> 10 μg/mL Compound: 4	Cytotoxicity against human HCT8 cells by tetrazolium salt-based colorimetric assay Cytotoxicity against human HCT8 cells by tetrazolium salt-based colorimetric assay	[PMID: 1431932]
KB	ED₅₀	> 10 μg/mL Compound: 4	Cytotoxicity against human KB cells by tetrazolium salt-based colorimetric assay Cytotoxicity against human KB cells by tetrazolium salt-based colorimetric assay	[PMID: 1431932]
TE-671	ED₅₀	> 10 μg/mL Compound: 4	Cytotoxicity against human TE671 cells by tetrazolium salt-based colorimetric assay Cytotoxicity against human TE671 cells by tetrazolium salt-based colorimetric assay	[PMID: 1431932]

In Vitro

β-Glucogallin (100 μM; 2 h) can reduce Methylglyoxal-induced oxidative stress in human retinal epithelial cells ARPE-19, decreasing intracellular ROS levels^[1].
β-Glucoside inhibits AKR1B1 with Glyceraldehyde (HY-128748) as substrate, with an IC₅₀ value of 58 μM^[2].
β-Glucogallin (30 μM; 72 h) can inhibit sorbitol accumulation under hyperglycemic conditions in a transgenic mouse lens organ culture model, with an inhibition rate of 73%^[2].
β-Glucogallin can inhibit the activity of AKR1B1 and prevent Sorbitol (HY-B0400) accumulation in RAW264.7 murine macrophage cells, with an inhibition rate of approximately 50%^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Real Time qPCR^[1]

Cell Line:	ARPE-19 (human retinal epithelial cell line)
Concentration:	100 μM
Incubation Time:	Pre-treated for 2 h
Result:	Decreased the expression levels of AR, αB-crystallin, PDGF, RAGE, and NF-κB.

In Vivo

β-Glucogallin (300-1200 mg/kg; p.o.; once daily; 10 days) improves Carbon tetrachloride (HY-Y0298)-induced hepatotoxicity in female Wistar rats^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Female Wistar rats (weight 60-80 g), Carbon tetrachloride-induced liver injury model^[4]
Dosage:	300 mg/kg, 600 mg/kg, 1200 mg/kg
Administration:	Oral gavage (p.o.), once daily for 10 days
Result:	Significantly increased the levels of hepatic GSH, SOD, catalase, and GPx. Reduced lipid peroxidation as indicated by the decrease in TBARS levels. Improved the levels of serum transaminases AST and ALT. Reduced central zone necrosis, vacuolation of hepatocytes, and inflammatory cell infiltration.

Molecular Weight

332.26

Formula

C₁₃H₁₆O₁₀

CAS No.

13405-60-2

Appearance

Solid

Color

White to off-white

SMILES

O[C@H]1[C@H](OC(C2=CC(O)=C(O)C(O)=C2)=O)O[C@H](CO)[C@@H](O)[C@@H]1O

Structure Classification

Initial Source

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (300.97 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : 6.25 mg/mL (18.81 mM; Need ultrasonic)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	3.0097 mL	15.0484 mL	30.0968 mL
5 mM	0.6019 mL	3.0097 mL	6.0194 mL
10 mM	0.3010 mL	1.5048 mL	3.0097 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (7.52 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (7.52 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (7.52 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.82%

Select Batch:

Data Sheet (280 KB) SDS (393 KB)

COA (251 KB) HNMR (228 KB) RP-HPLC (245 KB) MS (69 KB)

Handling Instructions (2659 KB)

References

[1]. Ma Y, Liu F, Xu Y. Protective Effect of β-Glucogallin on Damaged Cataract Against Methylglyoxal Induced Oxidative Stress in Cultured Lens Epithelial Cells. Med Sci Monit. 2019 Dec 7;25:9310-9318. [Content Brief]

[2]. Puppala M, Ponder J, Suryanarayana P, Reddy GB, Petrash JM, LaBarbera DV. The isolation and characterization of β-glucogallin as a novel aldose reductase inhibitor from Emblica officinalis. PLoS One. 2012;7(4):e31399. [Content Brief]

[3]. Li L, Chang KC, Zhou Y, Shieh B, Ponder J, Abraham AD, Ali H, Snow A, Petrash JM, LaBarbera DV. Design of an amide N-glycoside derivative of β-glucogallin: a stable, potent, and specific inhibitor of aldose reductase. J Med Chem. 2014 Jan 9;57(1):71-7. [Content Brief]

[4]. Majeed M, et al. Amelioration of carbon tetrachloride induced hepatotoxicity by Β-glucogallin, a gallic acid derivative of emblica officinalis gaertn.(euphorbiaceae). Science, 2015, 4(7): 696-701.

[5]. Li L, et al. Design of an amide N-glycoside derivative of β-glucogallin: a stable, potent, and specific inhibitor of aldose reductase. J Med Chem. 2014 Jan 9;57(1):71-7. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O / DMSO	1 mM	3.0097 mL	15.0484 mL	30.0968 mL	75.2421 mL
	5 mM	0.6019 mL	3.0097 mL	6.0194 mL	15.0484 mL
	10 mM	0.3010 mL	1.5048 mL	3.0097 mL	7.5242 mL
	15 mM	0.2006 mL	1.0032 mL	2.0065 mL	5.0161 mL
DMSO	20 mM	0.1505 mL	0.7524 mL	1.5048 mL	3.7621 mL
	25 mM	0.1204 mL	0.6019 mL	1.2039 mL	3.0097 mL
	30 mM	0.1003 mL	0.5016 mL	1.0032 mL	2.5081 mL
	40 mM	0.0752 mL	0.3762 mL	0.7524 mL	1.8811 mL
	50 mM	0.0602 mL	0.3010 mL	0.6019 mL	1.5048 mL
	60 mM	0.0502 mL	0.2508 mL	0.5016 mL	1.2540 mL
	80 mM	0.0376 mL	0.1881 mL	0.3762 mL	0.9405 mL
	100 mM	0.0301 mL	0.1505 mL	0.3010 mL	0.7524 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

β-Glucogallin Related Classifications

Neurological Disease Metabolic Disease

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Your Recently Viewed Products:

Product Name

Requested Quantity *

Applicant Name *

Salutation

Email Address *

Phone Number *

Department

Organization Name *

City

State

Country or Region *

Remarks

Product Name

Lot #

Applicant Name *

Email Address *

Phone Number

Department *

Organization Name *

Country or Region *

Remarks

Name
Email *

	Sorry, but the email address you supplied was invalid.

β-Glucogallin (Synonyms: 1-O-Galloyl-β-D-glucose)

Customer Review

β-Glucogallin Related Antibodies

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

View All NF-κB Isoform Specific Products:

View All PDGFR Isoform Specific Products:

Biological Activity

Purity & Documentation

References

Customer Review

β-Glucogallin Related Antibodies

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

β-Glucogallin Related Classifications

Keywords:

Your Recently Viewed Products:

Customer Review

Request for HNMR Report

SAFETY DATA SHEET (SDS)

Request for HNMR Report