Amine-PEG3-Biotin

Name: Amine-PEG3-Biotin
Brand: MedChemExpress
SKU: HY-111377
Rating: 4.5 (1 reviews)

Cat. No.: HY-111377 Purity: 98.41%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

Amine-PEG3-Biotin is a signal amplification label containing a biotin group and a terminal primary amine group.

For research use only. We do not sell to patients.

Amine-PEG3-Biotin Chemical Structure

CAS No. : 359860-27-8

Size	Price	Stock	Quantity
1 mg	USD 47	In-stock	Estimated Time of Arrival: December 31
5 mg	USD 60	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 97	In-stock	Estimated Time of Arrival: December 31
25 mg	USD 205	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 350	In-stock	Estimated Time of Arrival: December 31
100 mg	USD 500	In-stock	Estimated Time of Arrival: December 31
200 mg		Get quote
500 mg		Get quote

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Customer Review

Based on 1 publication(s) in Google Scholar

1 Publications Citing Use of MCE Amine-PEG3-Biotin

•Eur J Med Chem. 2020 Dec 1;207:112796. [Abstract]

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Biological Activity
Purity & Documentation
References
Customer Review

Description

Amine-PEG3-Biotin is a signal amplification label containing a biotin group and a terminal primary amine group^[1].

Molecular Weight

418.55

Formula

C₁₈H₃₄N₄O₅S

CAS No.

359860-27-8

Appearance

Solid

Color

White to off-white

SMILES

O=C(NCCOCCOCCOCCN)CCCC[C@@H]1SC[C@]([C@]1([H])N2)([H])NC2=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

-20°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (238.92 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : 100 mg/mL (238.92 mM; Need ultrasonic)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.3892 mL	11.9460 mL	23.8920 mL
5 mM	0.4778 mL	2.3892 mL	4.7784 mL
10 mM	0.2389 mL	1.1946 mL	2.3892 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 1.25 mg/mL (2.99 mM); Clear solution

This protocol yields a clear solution of ≥ 1.25 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (12.5 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 1.25 mg/mL (2.99 mM); Clear solution

This protocol yields a clear solution of ≥ 1.25 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (12.5 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 1.25 mg/mL (2.99 mM); Clear solution

This protocol yields a clear solution of ≥ 1.25 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (12.5 mg/mL) to 900 μL Corn oil, and mix evenly.

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: PBS
Solubility: 50 mg/mL (119.46 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Purity: 98.41%

Select Batch:

Data Sheet (268 KB) SDS (393 KB)

COA (242 KB) HNMR (301 KB) LCMS (88 KB)

Handling Instructions (2659 KB)

References

[1]. Yin H, et al. Electrochemical immunosensor for N6-methyladenosine detection in human cell lines based on biotin-streptavidin system and silver-SiO2 signal amplification. Biosens Bioelectron. 2017 Apr 15;90:494-500. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO / H₂O	1 mM	2.3892 mL	11.9460 mL	23.8920 mL	59.7300 mL
	5 mM	0.4778 mL	2.3892 mL	4.7784 mL	11.9460 mL
	10 mM	0.2389 mL	1.1946 mL	2.3892 mL	5.9730 mL
	15 mM	0.1593 mL	0.7964 mL	1.5928 mL	3.9820 mL
	20 mM	0.1195 mL	0.5973 mL	1.1946 mL	2.9865 mL
	25 mM	0.0956 mL	0.4778 mL	0.9557 mL	2.3892 mL
	30 mM	0.0796 mL	0.3982 mL	0.7964 mL	1.9910 mL
	40 mM	0.0597 mL	0.2987 mL	0.5973 mL	1.4933 mL
	50 mM	0.0478 mL	0.2389 mL	0.4778 mL	1.1946 mL
	60 mM	0.0398 mL	0.1991 mL	0.3982 mL	0.9955 mL
	80 mM	0.0299 mL	0.1493 mL	0.2987 mL	0.7466 mL
	100 mM	0.0239 mL	0.1195 mL	0.2389 mL	0.5973 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.