2. NF-κB Autophagy
  3. NF-κB Autophagy
  4. Berbamine

Berbamine is a natural compound extracted from traditional Chinese medicine Phellodendron amurense Rupr. with anti-tumor, immunomodulatory and cardiovascular effects. Berbamine is a calcium channel blocker.

For research use only. We do not sell to patients.

Berbamine Chemical Structure

Berbamine Chemical Structure

CAS No. : 478-61-5

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10 mM * 1 mL in DMSO
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10 mg USD 88 In-stock
20 mg USD 154 In-stock
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Customer Review

Based on 9 publication(s) in Google Scholar

Other Forms of Berbamine:

Berbamine Related Antibodies

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NF-κB NF-κB1/p50 RelA/p65 RelB c-Rel

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

Berbamine is a natural compound extracted from traditional Chinese medicine Phellodendron amurense Rupr. with anti-tumor, immunomodulatory and cardiovascular effects. Berbamine is a calcium channel blocker.

IC50 & Target[1]

NF-κB

 

In Vitro

Berbamine (8.17 μg/mL, 24 h) inhibits the growth of KM3 cells with an inhibition of 50%[1].
Berbamine (185.20 μg/mL, 48 h) induces apoptosis of normal hematopoietic cells with an inhibition of 50%[1].
Berbamine (8 μg/mL, 24 h) inhibits the growth of KM3 cells with a percentage of apoptotic cells is 14.32%[1].
Berbamine (8 μg/mL, 24 h) inhibits p65 nuclear translocation and expression of IKKα[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Berbamine Related Antibodies

Cell Viability Assay[1]

Cell Line: KM3 cell
Concentration: 1−32 μg/mL
Incubation Time: 24, 48, or 72 h
Result: Inhibited the growth of KM3 cells in a dose- and time-dependent manner.

Apoptosis Analysis[1]

Cell Line: KM3 cell
Concentration: 4 μg/mL
Incubation Time: 6, 12, or 24 h
Result: Induced apoptosis in a time-dependent manner treated at 8 μg/mL.

Western Blot Analysis[1]

Cell Line: KM3 cell
Concentration: 8 μg/mL
Incubation Time: 0, 6, 12, or 24 h
Result: Inhibited p65 nuclear translocation and expression of IKKα.
In Vivo

Berbamine (100 mg/kg, oral ) suppresses the growth of Huh7 xenografted tumors over time and leads to a tumor reduction of 70% based on the tumor weight[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Huh7 xenograft NOD/SCID mice model[2]
Dosage: 100mg/kg
Administration: Oral gavage (p.o.), twice a day for 5 consecutive days
Result: Suppressed tumor growth and reduced tumor weight by 70%.
Molecular Weight

608.72

Formula

C37H40N2O6
CAS No.
Appearance

Solid

Color

White to yellow

SMILES

COC1=C(OC2=CC([C@]3([H])CC(C=C4)=CC=C4O5)=C(CCN3C)C=C2OC)C([C@@]6([H])CC7=CC=C(O)C5=C7)=C(CCN6C)C=C1OC
Structure Classification
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (164.28 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.6428 mL 8.2140 mL 16.4279 mL
5 mM 0.3286 mL 1.6428 mL 3.2856 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.5 mg/mL (4.11 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% Corn Oil

    Solubility: ≥ 2.5 mg/mL (4.11 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation

Purity: 99.79%

COA (255 KB) HNMR (227 KB) LCMS (113 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 1.6428 mL 8.2140 mL 16.4279 mL 41.0698 mL
5 mM 0.3286 mL 1.6428 mL 3.2856 mL 8.2140 mL
10 mM 0.1643 mL 0.8214 mL 1.6428 mL 4.1070 mL
15 mM 0.1095 mL 0.5476 mL 1.0952 mL 2.7380 mL
20 mM 0.0821 mL 0.4107 mL 0.8214 mL 2.0535 mL
25 mM 0.0657 mL 0.3286 mL 0.6571 mL 1.6428 mL
30 mM 0.0548 mL 0.2738 mL 0.5476 mL 1.3690 mL
40 mM 0.0411 mL 0.2053 mL 0.4107 mL 1.0267 mL
50 mM 0.0329 mL 0.1643 mL 0.3286 mL 0.8214 mL
60 mM 0.0274 mL 0.1369 mL 0.2738 mL 0.6845 mL
80 mM 0.0205 mL 0.1027 mL 0.2053 mL 0.5134 mL
100 mM 0.0164 mL 0.0821 mL 0.1643 mL 0.4107 mL
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Berbamine Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Berbamine478-61-5NF-κBAutophagyNuclear factor-κBNuclear factor-kappaBInhibitorinhibitorinhibit

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