2. Immunology/Inflammation Vitamin D Related/Nuclear Receptor Metabolic Enzyme/Protease Membrane Transporter/Ion Channel Neuronal Signaling
  3. Glucocorticoid Receptor Endogenous Metabolite iGluR
  4. Corticosterone

Corticosterone  (Synonyms: 17-Deoxycortisol; 11β,21-Dihydroxyprogesterone; Kendall's compound B)

Cat. No.: HY-B1618 Purity: 99.72%
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Corticosterone (17-Deoxycortisol) is an orally active and adrenal cortex-produced glucocorticoid, which plays an important role in regulating neuronal functions of the limbic system (including hippocampus, prefrontal cortex, and amygdala). Corticosterone increases the Rab-mediated AMPAR membrane traffic via SGK-induced phosphorylation of GDI. Corticosterone also interferes with the maturation of dendritic cells and shows a good immunosuppressive effect.

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Corticosterone Chemical Structure

Corticosterone Chemical Structure

CAS No. : 50-22-6

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Based on 30 publication(s) in Google Scholar

Other Forms of Corticosterone:

Corticosterone Related Antibodies

Top Publications Citing Use of Products

    Corticosterone purchased from MedChemExpress. Usage Cited in: Sci Adv. 2022 Nov 11;8(45):eadd7063.  [Abstract]

    Corticosterone (CORT) (10 µM, 24 h) is used to produce a cell stress model on the primary culture hippocampal neurons from rats. The decreased NHE1 expression levels in primary cell culture induced by CORT treatment are not affected by chloroquine (Fig. D) but significantly reversed with MG132 pretreatment (Fig. E).

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    Description

    Corticosterone (17-Deoxycortisol) is an orally active and adrenal cortex-produced glucocorticoid, which plays an important role in regulating neuronal functions of the limbic system (including hippocampus, prefrontal cortex, and amygdala). Corticosterone increases the Rab-mediated AMPAR membrane traffic via SGK-induced phosphorylation of GDI. Corticosterone also interferes with the maturation of dendritic cells and shows a good immunosuppressive effect[1][2][3][4].

    IC50 & Target

    Human Endogenous Metabolite

     

    In Vitro

    Corticosterone (100 nM; 30 min) via SGK phosphorylation of GDI at Ser-213, increases the formation of GDI-Rab4 complex, facilitating the functional cycle of Rab4 and Rab4-mediated recycling of AMPARs to the synaptic membrane[1].
    Corticosterone (CORT) (1 µM; 48 h) shows good immunosuppressive properties (functionally compromises maturation of BMDC), which impairs LPS-induced up-regulation of maturation-associated markers (MHC class II, B7.2, B7.1 and CD40)[2].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Corticosterone Related Antibodies

    Cell Viability Assay[1]

    Cell Line: HEK293 cells
    Concentration: 100 nM
    Incubation Time: 30 min
    Result: Caused a significant enhancement of mEPSC amplitude (mEPSC represents the postsynaptic response to release of individual vesicles of glutamate).
    Increased the transmission of glutamatergic, and increased synaptic AMPAR currents via a Rab4-dependent mechanism.
    Profoundly increased surface GluR1 cluster density, cluster size and cluster fluorescence intensity.
    Significantly increased the amount of Rab4 that binded to WT-GDI, S45A-GDI, or S121A-GDI but not S213A-GDI.
    Induced the phosphorylation of GST-WTGDI, GST-S45AGDI, and GST-S121AGDI, but not GST-S213AGDI, and this effect was blocked in cells transfected with SGK1 small interfering RNA.
    Increased AMPAR surface expression via a mechanism dependent on GDI phosphorylation.

    Cell Viability Assay[2]

    Cell Line: BMDC cells
    Concentration: 1 µM
    Incubation Time: 48 h
    Result: Completely blocked the expression of MHC class II and B7.2 that induced by LPS, and maximally impaired BMDC cells maturation at 12 h.
    Reduced B7.1 by 50%, and slightly down-regulated CD40.
    In Vivo

    Corticosterone results in a marked reduction in the ability of BMDC cells to prime naive CD8+ T cells in vivo[2].
    Corticosterone (0.03 or 1 mg/kg; s.c.; single) downregulates expression of BDNF mRNA in dentate gyrus and CA1 of rats[3].
    Corticosterone (2.6 mg/kg; in animal feedings; 8 days) restores ethanol intake and preference to approximately normal preoperative levels in adrenalectomy (ADX) rats[4].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: Adult male Wistar rats (150-170 g; adrenalectomized)[3].
    Dosage: 0.03 or 1 mg/kg
    Administration: Subcutaneous injection; single.
    Result: Decreased expression of BDNF mRNA in dentate gyrus, with 25% and 50% lower for dosages of 0.03 and 1 mg/kg, respectively (3 h after administration).
    Reduced approximately 40% BDNF mRNA level as compared to the t=0 h control group (3 h after administration), but the level increased by 100% when 12 h after administration (compared to t=3 h and t=6 h group).
    Animal Model: Male Wistar rats (3-week-old; adrenalectomized)[4].
    Dosage: 2.6 mg/kg
    Administration: In animal feedings; 8 days.
    Result: Restored ethanol intake and preference of adrenalectomy (ADX) rats to approximately normal preoperative levels and to the levels observed in the sham-operated group (SH) rats.
    Clinical Trial
    Molecular Weight

    346.46

    Formula

    C21H30O4
    CAS No.
    Appearance

    Solid

    Color

    White to off-white

    SMILES

    C[C@@]12[C@@H](C(CO)=O)CC[C@@]1([H])[C@]3([H])CCC4=CC(CC[C@]4(C)[C@@]3([H])[C@@H](O)C2)=O
    Structure Classification
    Initial Source
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage
    Powder -20°C 3 years
    4°C 2 years
    In solvent -80°C 1 year
    -20°C 6 months
    Solvent & Solubility
    In Vitro: 

    DMSO : 100 mg/mL (288.63 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    Ethanol : 14.29 mg/mL (41.25 mM; Need ultrasonic)

    H2O : < 0.1 mg/mL (insoluble)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 2.8863 mL 14.4317 mL 28.8634 mL
    5 mM 0.5773 mL 2.8863 mL 5.7727 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

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    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

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    In Vivo:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 2.5 mg/mL (7.22 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

      Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
    • Protocol 2

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

      Solubility: ≥ 2.5 mg/mL (7.22 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

      Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.

    For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  0.5% CMC-Na/saline water

      Solubility: 2 mg/mL (5.77 mM); Suspended solution; Need ultrasonic and warming and heat to 60°C

    • Protocol 2

      Add each solvent one by one:  20% HP-β-CD in Saline

      Solubility: 4 mg/mL (11.55 mM); Suspended solution; Need ultrasonic

    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

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    Dosing volume
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    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Please enter your animal formula composition:
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    Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
    The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
    Calculation results:
    Working solution concentration: mg/mL
    Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
    Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
     If the continuous dosing period exceeds half a month, please choose this protocol carefully.
    Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
    Purity & Documentation

    Purity: 99.72%

    COA (252 KB) HNMR (252 KB) RP-HPLC (243 KB) LCMS (95 KB)

    Handling Instructions (2659 KB)
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    Ethanol / DMSO 1 mM 2.8863 mL 14.4317 mL 28.8634 mL 72.1584 mL
    5 mM 0.5773 mL 2.8863 mL 5.7727 mL 14.4317 mL
    10 mM 0.2886 mL 1.4432 mL 2.8863 mL 7.2158 mL
    15 mM 0.1924 mL 0.9621 mL 1.9242 mL 4.8106 mL
    20 mM 0.1443 mL 0.7216 mL 1.4432 mL 3.6079 mL
    25 mM 0.1155 mL 0.5773 mL 1.1545 mL 2.8863 mL
    30 mM 0.0962 mL 0.4811 mL 0.9621 mL 2.4053 mL
    40 mM 0.0722 mL 0.3608 mL 0.7216 mL 1.8040 mL
    DMSO 50 mM 0.0577 mL 0.2886 mL 0.5773 mL 1.4432 mL
    60 mM 0.0481 mL 0.2405 mL 0.4811 mL 1.2026 mL
    80 mM 0.0361 mL 0.1804 mL 0.3608 mL 0.9020 mL
    100 mM 0.0289 mL 0.1443 mL 0.2886 mL 0.7216 mL
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    Corticosterone Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Corticosterone50-22-617-Deoxycortisol 11β,21-Dihydroxyprogesterone Kendall's compound BGlucocorticoid ReceptorEndogenous MetaboliteiGluRIonotropic glutamate receptorsimmunosuppressionBMDCBDNFethanol intakeB7.2B7.1MHC class IICD40Inhibitorinhibitorinhibit

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