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  4. Dihydrofluorescein diacetate

Dihydrofluorescein diacetate is a fluorimetric probe mainly used for oxidative stress measurements, in both cell-free systems and cellular models.

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Dihydrofluorescein diacetate Chemical Structure

Dihydrofluorescein diacetate Chemical Structure

CAS No. : 35340-49-9

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Based on 1 publication(s) in Google Scholar

Dihydrofluorescein diacetate Related Antibodies

Top Publications Citing Use of Products

1 Publications Citing Use of MCE Dihydrofluorescein diacetate

  • Biological Activity

  • Protocol

  • Purity & Documentation

  • References

  • Customer Review

Description

Dihydrofluorescein diacetate is a fluorimetric probe mainly used for oxidative stress measurements, in both cell-free systems and cellular models.

In Vitro

Dihydrofluorescein diacetate may be a superior fluorescent probe for many cell-based studies. It is a better fluorescent probe for detecting intracellular oxidants because it is more reactive toward specific oxidizing species. Dihydrofluorescein diacetate demonstrates fluorescence of linear structures, consistent with mitochondria, in reoxygenated endothelium[1]. Dihydrofluorescein diacetate is able to detect the presence of ROS in mitochondria. Dihydrofluorescein diacetate fluorescence was sharp and delineated thin filaments which corresponded in all details to TMRM-stained mitochondria. It enters mitochondria and reacts with ROS released in the matrix[2]. Dihydrofluorescein diacetate could be an useful and quantitative method for measuring the oxidative potential of nanoparticle-treated cells[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Dihydrofluorescein diacetate Related Antibodies
Molecular Weight

418.40

Formula

C24H18O7
CAS No.
Appearance

Solid

Color

White to yellow

Emission (Em)

616
Excitation (Ex)

518
SMILES

O=C(O)C1=CC=CC=C1C2C3=C(OC4=C2C=CC(OC(C)=O)=C4)C=C(OC(C)=O)C=C3
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility
In Vitro: 

DMSO : ≥ 106.5 mg/mL (254.54 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.3901 mL 11.9503 mL 23.9006 mL
5 mM 0.4780 mL 2.3901 mL 4.7801 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation
Dyeing Example
References
Cell Assay

Dihydrofluorescein diacetate is used at a final concentration of 5 μM for the cell-free studies. The excitation wavelength is 488 nm and the emission wavelength is 521 nm, the same as the excitation and emission wavelengths of the confocal microscope. All fluorescence measurements are performed on room temperature solutions. Human umbilical vein endothelium at first passage are cultured to confluence on 48 well plates. Cells are ished with PBS, then PBS with glucose, pH 7.4, is added to the cells. Dihydrofluorescein diacetate is added at a final concentration of 20 mM. Wells without cells are also loaded with fluorescent probes to serve as controls. Fluorescence measurements are made on a fluorescent platereader every 10 min at Exλ 488 nm and Emλ 510 (bandpass 5 nm) and 538 nm (bandpass 10 nm). The Emλ 510 nm and Emλ 538 nm values are added together[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.3901 mL 11.9503 mL 23.9006 mL 59.7514 mL
5 mM 0.4780 mL 2.3901 mL 4.7801 mL 11.9503 mL
10 mM 0.2390 mL 1.1950 mL 2.3901 mL 5.9751 mL
15 mM 0.1593 mL 0.7967 mL 1.5934 mL 3.9834 mL
20 mM 0.1195 mL 0.5975 mL 1.1950 mL 2.9876 mL
25 mM 0.0956 mL 0.4780 mL 0.9560 mL 2.3901 mL
30 mM 0.0797 mL 0.3983 mL 0.7967 mL 1.9917 mL
40 mM 0.0598 mL 0.2988 mL 0.5975 mL 1.4938 mL
50 mM 0.0478 mL 0.2390 mL 0.4780 mL 1.1950 mL
60 mM 0.0398 mL 0.1992 mL 0.3983 mL 0.9959 mL
80 mM 0.0299 mL 0.1494 mL 0.2988 mL 0.7469 mL
100 mM 0.0239 mL 0.1195 mL 0.2390 mL 0.5975 mL
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Dihydrofluorescein diacetate Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Dihydrofluorescein diacetate35340-49-9Fluorescent DyeInhibitorinhibitorinhibit

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