Kinetin riboside (Synonyms: N6-Furfuryladenosine)

Name: Kinetin riboside
Brand: MedChemExpress
SKU: HY-101055
Rating: 5.0 (3 reviews)

Cat. No.: HY-101055 Purity: 99.88%: FAQs
Data Sheet SDS COA Handling Instructions

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Kinetin riboside, a cytokinin analog, can induce apoptosis in cancer cells. It inhibits the proliferation of HCT-15 cells with an IC₅₀ of 2.5 μM.

For research use only. We do not sell to patients.

Kinetin riboside Chemical Structure

CAS No. : 4338-47-0

Size	Price	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 59	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 59	In-stock	Estimated Time of Arrival: December 31
Solid
50 mg	USD 54	In-stock	Estimated Time of Arrival: December 31
100 mg	USD 84	In-stock	Estimated Time of Arrival: December 31
200 mg		Get quote
500 mg		Get quote

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Based on 3 publication(s) in Google Scholar

2 Publications Citing Use of MCE Kinetin riboside

: Kinetin riboside purchased from MedChemExpress. Usage Cited in: Int J Biol Sci. 2020 Jun 27;16(13):2382-2391. [Abstract]; After treatment of GES-1 cells with 100 nM glucocorticoids or chemicals for 48 h, the protein expression levels of ACE2 is examined, the Kinetin riboside exhibits an activating effect on ACE2 expression.

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Biological Activity
Protocol
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Description

Kinetin riboside, a cytokinin analog, can induce apoptosis in cancer cells. It inhibits the proliferation of HCT-15 cells with an IC₅₀ of 2.5 μM.

IC₅₀ & Target

IC50: 2.5 μM (HCT-15 cells)^[1]

Cellular Effect

Cell Line	Type	Value	Description	References
Caco-2	GI₅₀	45.6 μM Compound: 20, FMA	Antitumor activity against human CaCo2 cells after 48 hrs by MTS assay Antitumor activity against human CaCo2 cells after 48 hrs by MTS assay	[PMID: 18588281]
HT-29	GI₅₀	60.2 μM Compound: 20, FMA	Antitumor activity against human HT29 cells after 48 hrs by MTS assay Antitumor activity against human HT29 cells after 48 hrs by MTS assay	[PMID: 18588281]
K562	GI₅₀	3.3 μM Compound: 20, FMA	Antitumor activity against human K562 cells after 48 hrs by MTS assay Antitumor activity against human K562 cells after 48 hrs by MTS assay	[PMID: 18588281]
MCF7	GI₅₀	45.6 μM Compound: 20, FMA	Antitumor activity against human MCF7 cells after 48 hrs by MTS assay Antitumor activity against human MCF7 cells after 48 hrs by MTS assay	[PMID: 18588281]
Platelet	IC₅₀	41.16 μM Compound: 2c	Inhibition of collagen-induced human platelet aggregation after 3 mins by light transmission aggregometry Inhibition of collagen-induced human platelet aggregation after 3 mins by light transmission aggregometry	[PMID: 25467153]
RD	EC₅₀	1.4 μM Compound: 26	Antiviral activity against Enterovirus 71 infected in human RD cells assessed as cell viability after 3 days by MTS assay Antiviral activity against Enterovirus 71 infected in human RD cells assessed as cell viability after 3 days by MTS assay	[PMID: 26854380]

In Vitro

Kinetin riboside displays antiproliferative and apoptogenic activity against various human cancer cell lines. Kinetin riboside is able to inhibit the proliferation in HCT-15 human colon cancer cells in a dose-dependent manner (IC₅₀=2.5 μM)^[1]. Kinetin riboside induces apoptosis in HeLa and mouse melanoma B16F-10 cells. Kinetin riboside disrupts the mitochondrial membrane potential and induces the release of cytochrome c and activation of caspase-3. Bad are up-regulated while Bcl-2 is down-regulated under kinetin riboside exposure^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Kinetin riboside significantly suppresses tumor growth. The most effective anti-melanoma response is elicited at 40 mg/kg^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

347.33

Formula

C₁₅H₁₇N₅O₅

CAS No.

4338-47-0

Appearance

Solid

Color

White to off-white

SMILES

OC[C@@H]1[C@@H](O)[C@@H](O)[C@H](N2C3=NC=NC(NCC4=CC=CO4)=C3N=C2)O1

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

Solvent & Solubility

In Vitro:

DMSO : 250 mg/mL (719.78 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.8791 mL	14.3955 mL	28.7911 mL
5 mM	0.5758 mL	2.8791 mL	5.7582 mL
10 mM	0.2879 mL	1.4396 mL	2.8791 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (5.99 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.08 mg/mL (5.99 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.08 mg/mL (5.99 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.88%

Select Batch:

Data Sheet (278 KB) SDS (393 KB)

COA (252 KB) LCMS (93 KB)

Handling Instructions (2659 KB)

References

[1]. Rajabi M, et al. Antiproliferative activity of kinetin riboside on HCT-15 colon cancer cell line. Nucleosides Nucleotides Nucleic Acids. 2012;31(6):474-81. [Content Brief]

[2]. Choi BH, et al. Kinetin riboside preferentially induces apoptosis by modulating Bcl-2 family proteins and caspase-3 in cancer cells. Cancer Lett. 2008 Mar 8;261(1):37-45. [Content Brief]

Cell Assay ^[2]	HeLa and mouse melanoma B16F-10 cells are treated with 5, 10, 20 μM kinetin riboside for 48 h. 15 μL of MTT solution (5 mg/mL) is added to each well and cells are maintained for 4 h at 37°C. Hundred microlitres of solubilizing solution is then added. After an overnight incubation at room temperature, absorbance at 490 nm is measured^[2]. MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Animal Administration ^[2]	Mice: Male C57BL/6 mice are injected B16 F-10 cells. After 5 days for tumor growth, kinetin riboside (10, 20, 40 mg/kg) is injected to tumor mass directly. Drug injection is performed once a 3 days for three times. After third injection of drug, mice are kept for 3 days with no injection and tumor mass is removed from each mouse and weighed^[2]. MCE has not independently confirmed the accuracy of these methods. They are for reference only.
References	[1]. Rajabi M, et al. Antiproliferative activity of kinetin riboside on HCT-15 colon cancer cell line. Nucleosides Nucleotides Nucleic Acids. 2012;31(6):474-81. [Content Brief] [2]. Choi BH, et al. Kinetin riboside preferentially induces apoptosis by modulating Bcl-2 family proteins and caspase-3 in cancer cells. Cancer Lett. 2008 Mar 8;261(1):37-45. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.8791 mL	14.3955 mL	28.7911 mL	71.9777 mL
	5 mM	0.5758 mL	2.8791 mL	5.7582 mL	14.3955 mL
	10 mM	0.2879 mL	1.4396 mL	2.8791 mL	7.1978 mL
	15 mM	0.1919 mL	0.9597 mL	1.9194 mL	4.7985 mL
	20 mM	0.1440 mL	0.7198 mL	1.4396 mL	3.5989 mL
	25 mM	0.1152 mL	0.5758 mL	1.1516 mL	2.8791 mL
	30 mM	0.0960 mL	0.4799 mL	0.9597 mL	2.3993 mL
	40 mM	0.0720 mL	0.3599 mL	0.7198 mL	1.7994 mL
	50 mM	0.0576 mL	0.2879 mL	0.5758 mL	1.4396 mL
	60 mM	0.0480 mL	0.2399 mL	0.4799 mL	1.1996 mL
	80 mM	0.0360 mL	0.1799 mL	0.3599 mL	0.8997 mL
	100 mM	0.0288 mL	0.1440 mL	0.2879 mL	0.7198 mL

Kinetin riboside Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Kinetin riboside4338-47-0N6-FurfuryladenosineApoptosisInhibitorinhibitorinhibit

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Kinetin riboside (Synonyms: N6-Furfuryladenosine)

Customer Review

Kinetin riboside Related Antibodies

2 Publications Citing Use of MCE Kinetin riboside

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•Related Screening Libraries:

•Related Small Molecules:

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Biological Activity

Protocol

Purity & Documentation

References

Customer Review

Kinetin riboside Related Antibodies

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Complete Stock Solution Preparation Table

Kinetin riboside Related Classifications

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