2. Epigenetics Metabolic Enzyme/Protease NF-κB MAPK/ERK Pathway
  3. Methionine Adenosyltransferase (MAT) NF-κB p38 MAPK
  4. MAT2A inhibitor 2

MAT2A inhibitor 2 is an inhibitor of methionine adenosine transferase 2A (MAT2A) with oral activity. MAT2A inhibitor 2 can be used in osteoporosis study

For research use only. We do not sell to patients.

MAT2A inhibitor 2 Chemical Structure

MAT2A inhibitor 2 Chemical Structure

CAS No. : 13299-99-5

Size Price Stock Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 149 In-stock
Solution
10 mM * 1 mL in DMSO USD 149 In-stock
Solid
5 mg USD 135 In-stock
10 mg USD 210 In-stock
25 mg USD 380 In-stock
50 mg USD 570 In-stock
100 mg USD 850 In-stock
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Customer Review

Based on 1 publication(s) in Google Scholar

MAT2A inhibitor 2 Related Antibodies

Top Publications Citing Use of Products

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NF-κB NF-κB1/p50 RelA/p65 RelB c-Rel

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p38 MAPK p38α p38β MAPK13 p38δ p38γ

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

MAT2A inhibitor 2 is an inhibitor of methionine adenosine transferase 2A (MAT2A) with oral activity. MAT2A inhibitor 2 can be used in osteoporosis study[1]

IC50 & Target

MAT2A[1]
In Vitro

MAT2A inhibitor 2 (0.5, 1,2.5 μM, 48 h) inhibits ROS levels and the activation of NF-κB and MAPK pathways in osteoclasts[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

MAT2A inhibitor 2 Related Antibodies

Western Blot Analysis[1]

Cell Line: BMMs
Concentration: 0.5, 1, 2.5 μM
Incubation Time: 72 h
Result: Increased the protein levels of Nrf2, HO1, and NQO1.
Decreased phosphorylation level of p65, IκBα, p-p38, p-JNK and p-ERK.

Real Time qPCR[1]

Cell Line: BMMs
Concentration: 0.5, 1, 2.5 μM
Incubation Time: 48 h
Result: Inhibited the mRNA expression levels of NFATc1, c-FOS, TRAP, CTSK, and MMP9 in a concentration-dependent manner.
In Vivo

MAT2A inhibitor 2 (30, 100 mg/kg, orally, for 6 weeks) can prevent bone loss induced by ovariectomy (OVX) in mice[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Ovariectomy (OVX)-induced osteoporosis model in C57/BL mice[1]
Dosage: 30, 100 mg/kg
Administration: p.o. for 6 weeks
Result: Attenuated trabecular thickness and osteoclast surface/bone surface ratio (Oc.S/BS).
Molecular Weight

365.85

Formula

C18H24ClN3O3
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

O=C1C(C)=CC(C2=CC=CC(OC)=C2)=NN1CCN3CCOCC3.[H]Cl
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility
In Vitro: 

DMSO : 75 mg/mL (205.00 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.7334 mL 13.6668 mL 27.3336 mL
5 mM 0.5467 mL 2.7334 mL 5.4667 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.5 mg/mL (6.83 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.5 mg/mL (6.83 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.7334 mL 13.6668 mL 27.3336 mL 68.3340 mL
5 mM 0.5467 mL 2.7334 mL 5.4667 mL 13.6668 mL
10 mM 0.2733 mL 1.3667 mL 2.7334 mL 6.8334 mL
15 mM 0.1822 mL 0.9111 mL 1.8222 mL 4.5556 mL
20 mM 0.1367 mL 0.6833 mL 1.3667 mL 3.4167 mL
25 mM 0.1093 mL 0.5467 mL 1.0933 mL 2.7334 mL
30 mM 0.0911 mL 0.4556 mL 0.9111 mL 2.2778 mL
40 mM 0.0683 mL 0.3417 mL 0.6833 mL 1.7084 mL
50 mM 0.0547 mL 0.2733 mL 0.5467 mL 1.3667 mL
60 mM 0.0456 mL 0.2278 mL 0.4556 mL 1.1389 mL
80 mM 0.0342 mL 0.1708 mL 0.3417 mL 0.8542 mL
100 mM 0.0273 mL 0.1367 mL 0.2733 mL 0.6833 mL
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MAT2A inhibitor 2 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

MAT2A inhibitor 213299-99-5Methionine Adenosyltransferase (MAT)NF-κBp38 MAPKS-adenosylmethionine Synthetase Nuclear factor-κBNuclear factor-kappaBMAT2AInhibitorOsteoporosisBMMsOVXinhibitorinhibit

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