Biotin-C2-maleimide

Name: Biotin-C2-maleimide
Brand: MedChemExpress
SKU: HY-D1546
Rating: 4.5 (1 reviews)

Cat. No.: HY-D1546 Purity: 99.48%: FAQs
Data Sheet SDS COA Handling Instructions Technical Support

Biotin-C2-maleimide is an antibody conjugate reagent, can bind to many biomolecules without significantly changing the biological activity of the target molecule.

For research use only. We do not sell to patients.

Biotin-C2-maleimide Chemical Structure

CAS No. : 139554-72-6

Size	Stock	Quantity
5 mg	In-stock	Estimated Time of Arrival: December 31
10 mg	In-stock	Estimated Time of Arrival: December 31
25 mg	In-stock	Estimated Time of Arrival: December 31
50 mg	In-stock	Estimated Time of Arrival: December 31
100 mg	In-stock	Estimated Time of Arrival: December 31
200 mg	Get quote
500 mg	Get quote

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Based on 1 publication(s) in Google Scholar

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Biological Activity
Purity & Documentation
References
Customer Review

Description

Biotin-C2-maleimide is an antibody conjugate reagent, can bind to many biomolecules without significantly changing the biological activity of the target molecule^[1]^[2].

In Vitro

Biotin-C2-maleimide can act bioconjugation with 5 eq. SUMO-1 probes^[1].
1. Biotin C2 maleimide (ATT Bioquest, dissolved in DMSO) is performed in Tris buffer (50 mM Tris, 300 mM NaCl; pH 7.0) after 15 min reduction with Tris (2-carboxyethyl)phosphine (TCEP, 120 μM) at room temperature.
2. After 1 h incubation, the unreacted Biotin is separated by Ni-NTA affinity chromatography followed by dialysis against PBS buffer (pH 7.4).

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

366.44

Formula

C₁₆H₂₂N₄O₄S

CAS No.

139554-72-6

Appearance

Solid

Color

White to off-white

SMILES

O=C(NCCN1C(C=CC1=O)=O)CCCC[C@@H]2SC[C@]([C@]2([H])N3)([H])NC3=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

-20°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility

In Vitro:

DMSO : 125 mg/mL (341.12 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.7290 mL	13.6448 mL	27.2896 mL
5 mM	0.5458 mL	2.7290 mL	5.4579 mL
10 mM	0.2729 mL	1.3645 mL	2.7290 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

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Concentration _(final)

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In Vivo Dissolution Calculator

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Calculation results:

Working solution concentration: mg/mL

Purity & Documentation

Purity: 99.48%

Select Batch:

Data Sheet (271 KB) SDS (393 KB)

COA (248 KB) HNMR (285 KB) RP-HPLC (248 KB) MS (69 KB)

Handling Instructions (2659 KB)

References

[1]. Benjamin GD, et al. Antibody conjugates and methods of making the antibody conjugates: United States, US20160361436A1. 2016-12-15.

[2]. Brüninghoff K, Wulff S, Dörner W, Geiss-Friedlander R, Mootz HD. A Photo-Crosslinking Approach to Identify Class II SUMO-1 Binders. Front Chem. 2022 May 30;10:900989. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.7290 mL	13.6448 mL	27.2896 mL	68.2240 mL
	5 mM	0.5458 mL	2.7290 mL	5.4579 mL	13.6448 mL
	10 mM	0.2729 mL	1.3645 mL	2.7290 mL	6.8224 mL
	15 mM	0.1819 mL	0.9097 mL	1.8193 mL	4.5483 mL
	20 mM	0.1364 mL	0.6822 mL	1.3645 mL	3.4112 mL
	25 mM	0.1092 mL	0.5458 mL	1.0916 mL	2.7290 mL
	30 mM	0.0910 mL	0.4548 mL	0.9097 mL	2.2741 mL
	40 mM	0.0682 mL	0.3411 mL	0.6822 mL	1.7056 mL
	50 mM	0.0546 mL	0.2729 mL	0.5458 mL	1.3645 mL
	60 mM	0.0455 mL	0.2274 mL	0.4548 mL	1.1371 mL
	80 mM	0.0341 mL	0.1706 mL	0.3411 mL	0.8528 mL
	100 mM	0.0273 mL	0.1364 mL	0.2729 mL	0.6822 mL