2. Apoptosis
  3. Apoptosis
  4. M47

M47 is a small molecule that selectively destabilizes Cryptochrome 1 (CRY1) and increases degradation of the CRY1 in the nucleus. M47 enhances apoptosis in Ras-transformed P53-deficient mouse skin fibroblast lines and enhances life span in p53 knockout mice. M47 can be used in research of cancer.

For research use only. We do not sell to patients.

M47 Chemical Structure

M47 Chemical Structure

CAS No. : 890808-56-7

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Based on 1 publication(s) in Google Scholar

M47 Related Antibodies

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Description

M47 is a small molecule that selectively destabilizes Cryptochrome 1 (CRY1) and increases degradation of the CRY1 in the nucleus. M47 enhances apoptosis in Ras-transformed P53-deficient mouse skin fibroblast lines and enhances life span in p53 knockout mice. M47 can be used in research of cancer[1].

In Vitro

M47 (0-10 μM; 0-120 h; U2OS Bmal1-dLuc cells) reduces the half-life of CRY1 in a dose-dependent manner and causes circadian changes[1].
M47 (10 μM; 24-44 h; U2OS cells) decreases the level of CRY1 in U2OS cells[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

M47 Related Antibodies

Western Blot Analysis[1]

Cell Line: U2OS cells
Concentration: 10 μM
Incubation Time: 24-44 hours
Result: Decreased CRY1 protein levels between 24 and 48 h.
In Vivo

M47 (5-1000 mg/kg; i.p.; daily, for 5 d; C57BL/6 J mice and p53 gene knockout C57BL/6 J mice) selectively reduces the half-life of CRY1 and is well tolerated with a good pharmacokinetic profile in vivo and enhances apoptosis[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: C57BL/6 J mice and p53 gene knockout C57BL/6 J mice[1]
Dosage: 5, 50, 300 and 1000 mg/kg
Administration: intraperitoneal injection, daily, for 5 days
Result: Prolonged the life span of p53 gene knockout mice.
Molecular Weight

471.93

Formula

C28H22ClNO4
CAS No.
Appearance

Solid

Color

Light yellow to light brown

SMILES

O=C(N1C2=C(CC1)C=CC=C2)C(OC3=CC=C4OC(C)(C5)C6=CC=C(C=C6)Cl)=C(C)C3=C4C5=O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 6.5 mg/mL (13.77 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.1190 mL 10.5948 mL 21.1896 mL
5 mM 0.4238 mL 2.1190 mL 4.2379 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

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In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation

Purity: 99.90%

SDS (393 KB)

COA (250 KB) LCMS (153 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.1190 mL 10.5948 mL 21.1896 mL 52.9740 mL
5 mM 0.4238 mL 2.1190 mL 4.2379 mL 10.5948 mL
10 mM 0.2119 mL 1.0595 mL 2.1190 mL 5.2974 mL
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M47 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

M47890808-56-7M 47M-47ApoptosisCRY1nucleusdegradationcancerp53U2OS Bmal1-dLuc cellsInhibitorinhibitorinhibit

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