N6-(2-Hydroxyethyl)adenosine

Name: N6-(2-Hydroxyethyl)adenosine
Brand: MedChemExpress
SKU: HY-W006957
Rating: 4.5 (1 reviews)

Cat. No.: HY-W006957 Purity: 99.76%: Data Sheet
SDS

COA
Handling Instructions Technical Support

Solubility

In Vivo Dissolution Calculator

N6-(2-Hydroxyethyl)adenosine is a purine nucleoside analog. N6-(2-Hydroxyethyl)adenosine inhibits NF-κB/Smad signaling pathway, exhibits anti-hyperglycemia, antioxidant, antitumor and anti-inflammatory and insecticidal activities. N6-(2-Hydroxyethyl)adenosine is orally active.

For research use only. We do not sell to patients.

N6-(2-Hydroxyethyl)adenosine Chemical Structure

CAS No. : 4338-48-1

Size	Price	Stock	Quantity

Free Sample (0.1 - 0.5 mg)		Apply Now
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 42	In-stock	Estimated Time of Arrival: April 29
Solution
10 mM * 1 mL in DMSO	USD 44	In-stock	Estimated Time of Arrival: April 29
Solid
50 mg	USD 40	In-stock	Estimated Time of Arrival: April 29
100 mg	USD 60	In-stock	Estimated Time of Arrival: April 29
200 mg		Get quote
500 mg		Get quote

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Customer Review

Based on 1 publication(s) in Google Scholar

1 Publications Citing Use of MCE N6-(2-Hydroxyethyl)adenosine

•Clin Transl Med. 2023 Jul;13(7):e1336. [Abstract]

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•Related Screening Libraries:

•Related Small Molecules:

View All NF-κB Isoform Specific Products:

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NF-κB NF-κB1/p50 RelA/p65 RelB c-Rel

Biological Activity
Purity & Documentation
References
Customer Review

Description

In Vitro

N6-(2-Hydroxyethyl)adenosine (5-20 µg/mL, 24 h) reduces the expression of LPS (HY0D1056)-induced TNF-α and IL-1β, increases the IL-10 secretion in RAW 264.7 cell^[3].
N6-(2-Hydroxyethyl)adenosine (5-20 µg/mL, 24 h) reduces TGF-β1-induced expression of collagen I, α-SMA and fibronectin in NRK-49F cell, exhibits anti-fibrosis effect^[3].
N6-(2-Hydroxyethyl)adenosine (0-300 µM, 24-72 h) induces the generation of ROS, decreases mitochondrial membrane potential, exhibits cytotoxicity in cancer cells SGC-7901 and AGS, and induces apoptosis and autophagy^[4].
N6-(2-Hydroxyethyl)adenosine (1 mg/mL, 60 h) exhibits insecticidal activity against Plutella xylostella^[5].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[4]

Cell Line:	SGC-7901 and AGS
Concentration:	0-300 µM
Incubation Time:	72 h
Result:	Inhibited cell viability.

Apoptosis Analysis^[4]

Cell Line:	SGC-7901 and AGS
Concentration:	0-150 µM
Incubation Time:	48 h
Result:	Induced apoptosis.

Real Time qPCR^[3]

Cell Line:	RAW 264.7 and NRK-49F
Concentration:	5-20 µg/mL
Incubation Time:	24 h
Result:	Inhibited the expression of TNF-α, IL-1β, collagen I, α-SMA and fibronectin. Increased the expression of IL-10.

In Vivo

N6-(2-Hydroxyethyl)adenosine (20-40 mg/kg, ip, once daily for 6 weeks) reduces the blood glucose level and diabetes-induced kidney damage, increases the activities of antioxidant enzymes (such as SOD, catalase CAT and GSH) in renal tissue, reduces the level of malondialdehyde (MDA), exhibits anti-inflammatory and antioxidant activities, and ameliorates Alloxan (HY-W017227)-induced diabetes in rats models^[2].
N6-(2-Hydroxyethyl)adenosine (75-150 mg/kg, po, once daily for 19 days) exhibits antitumor effect in SGC-7901 xenograft mouse models^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Alloxan-induced diabetes in rats models^[2]
Dosage:	20-40 mg/kg
Administration:	ip, once daily for 6 weeks
Result:	Reduced the levels of blood glucose, serum creatinine (Scr), blood urea nitrogen (BUN) and urine protein. Reduced the levels of TNF-α, IL-1β, IL-6, TGF-β1 and NF-κB in kidney tissue. Alleviated the damage of glomeruli and renal tubules.

Animal Model:	SGC-7901 xenograft mouse models^[4]
Dosage:	75-150 mg/kg
Administration:	po, once daily for 19 days
Result:	Reduced tumor volume and weight.

Molecular Weight

311.30

Formula

C₁₂H₁₇N₅O₅

CAS No.

4338-48-1

Appearance

Solid

Color

White to off-white

SMILES

OCCNC1=NC=NC2=C1N=CN2[C@H]3[C@H](O)[C@H](O)[C@@H](CO)O3

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (321.24 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	3.2124 mL	16.0618 mL	32.1236 mL
5 mM	0.6425 mL	3.2124 mL	6.4247 mL
10 mM	0.3212 mL	1.6062 mL	3.2124 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (8.03 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (8.03 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (8.03 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, PEG300/PEG400, Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Purity & Documentation

Purity: 99.76%

Select Batch:

Data Sheet (282 KB) SDS (393 KB)

COA (250 KB) HNMR (126 KB) RP-HPLC (275 KB)

Handling Instructions (2659 KB)

References

[1]. Man S, et al. Potential and promising anticancer drugs from adenosine and its analogs. Drug Discov Today. 2021 Jun;26(6):1490-1500. [Content Brief]

[2]. Wang X, et al., N6 -(2-hydroxyethyl)-adenosine from Cordyceps cicadae protects against diabetic kidney disease via alleviation of oxidative stress and inflammation. J Food Biochem. 2019 Feb;43(2):e12727. [Content Brief]

[3]. Zheng R, et al., N6-(2-Hydroxyethyl) Adenosine From Cordyceps cicadae Ameliorates Renal Interstitial Fibrosis and Prevents Inflammation via TGF-β1/Smad and NF-κB Signaling Pathway. Front Physiol. 2018 Sep 4;9:1229. [Content Brief]

[4]. Xie H, et al., N6-(2-hydroxyethyl)-Adenosine Induces Apoptosis via ER Stress and Autophagy of Gastric Carcinoma Cells In Vitro and In Vivo. Int J Mol Sci. 2020 Aug 13;21(16):5815. [Content Brief]

[5]. Fang M, et al., N6-(2-Hydroxyethyl)-Adenosine Exhibits Insecticidal Activity against Plutella xylostella via Adenosine Receptors. PLoS One. 2016 Sep 26;11(9):e0162859. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	3.2124 mL	16.0618 mL	32.1236 mL	80.3089 mL
	5 mM	0.6425 mL	3.2124 mL	6.4247 mL	16.0618 mL
	10 mM	0.3212 mL	1.6062 mL	3.2124 mL	8.0309 mL
	15 mM	0.2142 mL	1.0708 mL	2.1416 mL	5.3539 mL
	20 mM	0.1606 mL	0.8031 mL	1.6062 mL	4.0154 mL
	25 mM	0.1285 mL	0.6425 mL	1.2849 mL	3.2124 mL
	30 mM	0.1071 mL	0.5354 mL	1.0708 mL	2.6770 mL
	40 mM	0.0803 mL	0.4015 mL	0.8031 mL	2.0077 mL
	50 mM	0.0642 mL	0.3212 mL	0.6425 mL	1.6062 mL
	60 mM	0.0535 mL	0.2677 mL	0.5354 mL	1.3385 mL
	80 mM	0.0402 mL	0.2008 mL	0.4015 mL	1.0039 mL
	100 mM	0.0321 mL	0.1606 mL	0.3212 mL	0.8031 mL