1. Anti-infection Autophagy
  2. Parasite Influenza Virus Autophagy
  3. Nitazoxanide

Nitazoxanide  (Synonyms: NTZ; NSC 697855)

Cat. No.: HY-B0217 Purity: 99.28%
Data Sheet Handling Instructions Technical Support

Nitazoxanide (NTZ), an anthelmintic agent, exhibits a broad spectrum of activities against a wide variety of helminths, protozoa, and enteric bacteria infecting animals and humans. Nitazoxanide inhibits Giardia lamblia trophozoite proliferation in axenic culture with an IC50 of 2.4 μM. Nitazoxanide can be used for the research of parasitic gastroenteritis. Nitazoxanide shows anti-Japanese encephalitis virus (JEV) activity in a mouse model.

For research use only. We do not sell to patients.

Nitazoxanide Chemical Structure

Nitazoxanide Chemical Structure

CAS No. : 55981-09-4

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Customer Review

Based on 12 publication(s) in Google Scholar

Other Forms of Nitazoxanide:

Top Publications Citing Use of Products
  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

Nitazoxanide (NTZ), an anthelmintic agent, exhibits a broad spectrum of activities against a wide variety of helminths, protozoa, and enteric bacteria infecting animals and humans. Nitazoxanide inhibits Giardia lamblia trophozoite proliferation in axenic culture with an IC50 of 2.4 μM[1]. Nitazoxanide can be used for the research of parasitic gastroenteritis. Nitazoxanide shows anti-Japanese encephalitis virus (JEV) activity in a mouse model[2].

Cellular Effect
Cell Line Type Value Description References
A549 IC50
> 50 μM
Compound: NTZ
Antiproliferative activity against human A549 cells assessed as cell growth inhibition measured after 48 hrs by MTT assay
Antiproliferative activity against human A549 cells assessed as cell growth inhibition measured after 48 hrs by MTT assay
[PMID: 34055214]
BHK-21 CC50
25 μM
Compound: 105
Cytotoxicity against BHK21 cells assessed as reduction in cell viability after 16 hrs by CCK-8 assay
Cytotoxicity against BHK21 cells assessed as reduction in cell viability after 16 hrs by CCK-8 assay
[PMID: 28689975]
Caco-2 IC50
221 μM
Compound: Nitazoxanide
Antiprolferative activity against human Caco2 cells assessed as inhibition of cell proliferation by measuring [3H]-thymidine incorporation incubated for 48 hrs by liquid scintillometry
Antiprolferative activity against human Caco2 cells assessed as inhibition of cell proliferation by measuring [3H]-thymidine incorporation incubated for 48 hrs by liquid scintillometry
[PMID: 28601526]
Caco-2 IC50
26.8 μM
Compound: NTZ
Antiproliferative activity against human Caco-2 cells assessed as cell growth inhibition measured after 48 hrs by MTT assay
Antiproliferative activity against human Caco-2 cells assessed as cell growth inhibition measured after 48 hrs by MTT assay
[PMID: 34055214]
HEK-293T CC50
> 40 μM
Compound: Nitazoxanide
Cytotoxicity against human HEK293T cells assessed as decrease in cell viability after 72 hrs by CellTiter-Glo reagent based luminescence assay
Cytotoxicity against human HEK293T cells assessed as decrease in cell viability after 72 hrs by CellTiter-Glo reagent based luminescence assay
[PMID: 29469575]
HeLa IC50
35 μM
Compound: NTZ
Cytotoxicity against human HeLa cells assessed as inhibition of cell viability measured after 48 hrs by MTT assay
Cytotoxicity against human HeLa cells assessed as inhibition of cell viability measured after 48 hrs by MTT assay
[PMID: 34055214]
HeLa IC50
35 μM
Compound: NTZ
Antiproliferative activity against human HeLa cells assessed as cell growth inhibition measured after 48 hrs by MTT assay
Antiproliferative activity against human HeLa cells assessed as cell growth inhibition measured after 48 hrs by MTT assay
[PMID: 34055214]
HepG2 CC50
> 40 μM
Compound: Nitazoxanide
Cytotoxicity against human HepG2 cells assessed as decrease in cell viability after 72 hrs by CellTiter-Glo reagent based luminescence assay
Cytotoxicity against human HepG2 cells assessed as decrease in cell viability after 72 hrs by CellTiter-Glo reagent based luminescence assay
[PMID: 29469575]
HepG2 2.2.15 CC50
> 100 μM
Compound: 1, NTZ
Cytotoxicity against human HepG2(2.2.15) cells after 24 hrs by neutral red dye uptake assay
Cytotoxicity against human HepG2(2.2.15) cells after 24 hrs by neutral red dye uptake assay
[PMID: 21553812]
HL-60 IC50
20.1 μM
Compound: NTZ
Antiproliferative activity against human HL60 cells assessed as cell growth inhibition measured after 48 hrs by MTT assay
Antiproliferative activity against human HL60 cells assessed as cell growth inhibition measured after 48 hrs by MTT assay
[PMID: 34055214]
HT-29 IC50
> 50 μM
Compound: NTZ
Antiproliferative activity against human HT29 cells assessed as cell growth inhibition measured after 48 hrs by MTT assay
Antiproliferative activity against human HT29 cells assessed as cell growth inhibition measured after 48 hrs by MTT assay
[PMID: 34055214]
Huh-7 CC50
49 μM
Compound: 1, NTZ, PH-5776
Cytotoxicity against human Huh7.5 cells after 3 days by neutral red dye assay
Cytotoxicity against human Huh7.5 cells after 3 days by neutral red dye assay
[PMID: 22059983]
PC-3 IC50
44.7 μM
Compound: NTZ
Antiproliferative activity against human PC3 cells assessed as cell growth inhibition measured after 48 hrs by MTT assay
Antiproliferative activity against human PC3 cells assessed as cell growth inhibition measured after 48 hrs by MTT assay
[PMID: 34055214]
Peritoneal macrophage IC50
2 mM
Compound: 36, NTZ
Anti-inflammatory activity against mouse peritoneal macrophages assessed as reduction in LPS induced IL-6 level by ELISA analysis
Anti-inflammatory activity against mouse peritoneal macrophages assessed as reduction in LPS induced IL-6 level by ELISA analysis
[PMID: 32992245]
RAW264.7 IC50
1.54 mM
Compound: 36, NTZ
Anti-inflammatory activity against mouse RAW264.7 cells assessed as reduction in LPS induced IL-6 level by ELISA analysis
Anti-inflammatory activity against mouse RAW264.7 cells assessed as reduction in LPS induced IL-6 level by ELISA analysis
[PMID: 32992245]
U2OS CC50
25 μM
Compound: 105
Cytotoxicity against human U2OS cells assessed as reduction in cell viability after 16 hrs by CCK-8 assay
Cytotoxicity against human U2OS cells assessed as reduction in cell viability after 16 hrs by CCK-8 assay
[PMID: 28689975]
Vero IC50
10.74 μM
Compound: NTZ
Cytotoxicity against african green monkey Vero cells assessed as cell viability after 48 hrs by WST-1 assay
Cytotoxicity against african green monkey Vero cells assessed as cell viability after 48 hrs by WST-1 assay
[PMID: 23787289]
Vero CC50
833 μM
Compound: Nitazoxanide
Cytotoxicity against African green monkey Vero cells after 48 hrs by MTT assay
Cytotoxicity against African green monkey Vero cells after 48 hrs by MTT assay
[PMID: 25801157]
Vero CC50
833 μM
Compound: NIT, Alinia
Cytotoxicity against african green monkey Vero cells after 72 hrs by MTT assay
Cytotoxicity against african green monkey Vero cells after 72 hrs by MTT assay
[PMID: 21397502]
Vero CC50
833 μM
Compound: Nitazoxanide
Cytotoxicity against African green monkey Vero cells incubated for 24 to 48 hrs by SRB assay
Cytotoxicity against African green monkey Vero cells incubated for 24 to 48 hrs by SRB assay
[PMID: 28645659]
Vero CC50
833 μM
Compound: NTZ
Cytotoxicity against African green monkey Vero cells assessed as cell viability after 48 hrs by SRB assay
Cytotoxicity against African green monkey Vero cells assessed as cell viability after 48 hrs by SRB assay
[PMID: 24529307]
Vero C1008 CC50
> 35.5 μM
Compound: 48
Cytotoxicity against African green monkey Vero E6 cells by the CCK8 assay
Cytotoxicity against African green monkey Vero E6 cells by the CCK8 assay
[PMID: 32845145]
In Vitro

Giardia lamblia, a flagellated protozoan, is the most common causative agent of persistent diarrhea worldwide[1].
Nitazoxanide exhibits effect on G. lamblia trophozoite proliferation in axenic culture with an IC50 of 2.4 μM[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay[1]

Cell Line: Human cancer colon Caco2 cells were incubated with increasing numbers of Giardia lamblia trophozoites (103 to 106 parasites per well)
Concentration: 30 μM
Incubation Time: 24 hours
Result: 70 to 90% of the trophozoites remained attached to the Caco2 cells for a period of 24 to 48 h in the absence of Nitazoxanide and at an initial inoculum density of 105 parasites per well.
The number of parasites still attached to Caco2 cells after 24 h decreased to less than 20% of the control value in the presence of 30 μM Nitazoxanide with an inoculum density of 105 trophozoites.
In Vivo

Nitazoxanide exhibits a wide spectrum of in vivo activity against a broad spectrum of intestinal parasites, such as Giardia lamblia, Entamoeba histolytica, Trichomonas vaginalis, the apicomplexan Cryptosporidium parvum, and enteric bacteria infecting animals and humans[1].
Nitazoxanide (50, 75 or 100 mg/kg/day; administered daily by intragastric for up to 25 days) reduces the mortality of Japanese encephalitis virus (JEV) strain-infected mice and protected mice from a lethal dose challenge of JEV[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Three-week old female Chinese Kunming mice (12–14 g body weight) were infected intraperitoneally with JEV[2]
Dosage: 50, 75 or 100 mg/kg/day
Administration: Administered intragastrically by gavage
Result: 50 mg/kg/day, 75 mg/kg/day and 100 mg/kg/day led to 30%, 70% and 90% mice survival, respectively.
Molecular Weight

307.28

Formula

C12H9N3O5S

CAS No.
Appearance

Solid

Color

Light yellow to yellow

SMILES

O=C(NC1=NC=C([N+]([O-])=O)S1)C2=CC=CC=C2OC(C)=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 2 years
-20°C 1 year
Solvent & Solubility
In Vitro: 

DMSO : ≥ 100 mg/mL (325.44 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.2544 mL 16.2718 mL 32.5436 mL
5 mM 0.6509 mL 3.2544 mL 6.5087 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
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Concentration
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Volume
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Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

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Volume (start)

V1

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C2

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In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 3.25 mg/mL (10.58 mM); Clear solution

    This protocol yields a clear solution of ≥ 3.25 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (32.5 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, PEG300/PEG400, Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 3.2544 mL 16.2718 mL 32.5436 mL 81.3590 mL
5 mM 0.6509 mL 3.2544 mL 6.5087 mL 16.2718 mL
10 mM 0.3254 mL 1.6272 mL 3.2544 mL 8.1359 mL
15 mM 0.2170 mL 1.0848 mL 2.1696 mL 5.4239 mL
20 mM 0.1627 mL 0.8136 mL 1.6272 mL 4.0680 mL
25 mM 0.1302 mL 0.6509 mL 1.3017 mL 3.2544 mL
30 mM 0.1085 mL 0.5424 mL 1.0848 mL 2.7120 mL
40 mM 0.0814 mL 0.4068 mL 0.8136 mL 2.0340 mL
50 mM 0.0651 mL 0.3254 mL 0.6509 mL 1.6272 mL
60 mM 0.0542 mL 0.2712 mL 0.5424 mL 1.3560 mL
80 mM 0.0407 mL 0.2034 mL 0.4068 mL 1.0170 mL
100 mM 0.0325 mL 0.1627 mL 0.3254 mL 0.8136 mL
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Nitazoxanide
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