2. Anti-infection Autophagy
  3. Parasite Influenza Virus Autophagy
  4. Nitazoxanide

Nitazoxanide  (Synonyms: NTZ; NSC 697855)

Cat. No.: HY-B0217 Purity: 99.95%
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Nitazoxanide (NTZ), an anthelmintic agent, exhibits a broad spectrum of activities against a wide variety of helminths, protozoa, and enteric bacteria infecting animals and humans. Nitazoxanide inhibits Giardia lamblia trophozoite proliferation in axenic culture with an IC50 of 2.4 μM. Nitazoxanide can be used for the research of parasitic gastroenteritis. Nitazoxanide shows anti-Japanese encephalitis virus (JEV) activity in a mouse model.

For research use only. We do not sell to patients.

Nitazoxanide Chemical Structure

Nitazoxanide Chemical Structure

CAS No. : 55981-09-4

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Based on 12 publication(s) in Google Scholar

Other Forms of Nitazoxanide:

Nitazoxanide Related Antibodies

Top Publications Citing Use of Products

    Nitazoxanide purchased from MedChemExpress. Usage Cited in: Cell Death Dis. 2018 Oct 9;9(10):1032.  [Abstract]

    Western blot showing SQSTM1 and LC3 levels in LN229 cells after treatment with 200 μM Nitazoxanide (NTZ) and 200 nM Bafilomycin A1 (BAF) for 48 h.

    Nitazoxanide purchased from MedChemExpress. Usage Cited in: Cell Death Dis. 2018 Oct 9;9(10):1032.  [Abstract]

    Western blot showing the cell cycle and autophagy-related proteins after 48 h of treatment with 200 μM Nitazoxanide (NTZ) and/or 500 nM Torin 1 or 30 mM Chloroquine (CQ).

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    • Biological Activity

    • Purity & Documentation

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    Description

    Nitazoxanide (NTZ), an anthelmintic agent, exhibits a broad spectrum of activities against a wide variety of helminths, protozoa, and enteric bacteria infecting animals and humans. Nitazoxanide inhibits Giardia lamblia trophozoite proliferation in axenic culture with an IC50 of 2.4 μM[1]. Nitazoxanide can be used for the research of parasitic gastroenteritis. Nitazoxanide shows anti-Japanese encephalitis virus (JEV) activity in a mouse model[2].

    In Vitro

    Giardia lamblia, a flagellated protozoan, is the most common causative agent of persistent diarrhea worldwide[1].
    ? Nitazoxanide exhibits effect on G. lamblia trophozoite proliferation in axenic culture with an IC50 of 2.4 μM[1].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Nitazoxanide Related Antibodies

    Cell Viability Assay[1]

    Cell Line: Human cancer colon Caco2 cells were incubated with increasing numbers of Giardia lamblia trophozoites (103 to 106 parasites per well)
    Concentration: 30 μM
    Incubation Time: 24 hours
    Result: 70 to 90% of the trophozoites remained attached to the Caco2 cells for a period of 24 to 48 h in the absence of Nitazoxanide and at an initial inoculum density of 105 parasites per well.
    The number of parasites still attached to Caco2 cells after 24 h decreased to less than 20% of the control value in the presence of 30 μM Nitazoxanide with an inoculum density of 105 trophozoites.
    In Vivo

    Nitazoxanide exhibits a wide spectrum of in vivo activity against a broad spectrum of intestinal parasites, such as Giardia lamblia, Entamoeba histolytica, Trichomonas vaginalis, the apicomplexan Cryptosporidium parvum, and enteric bacteria infecting animals and humans[1].
    ? Nitazoxanide (50, 75 or 100 mg/kg/day; administered daily by intragastric for up to 25 days) reduces the mortality of Japanese encephalitis virus (JEV) strain-infected mice and protected mice from a lethal dose challenge of JEV[2].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: Three-week old female Chinese Kunming mice (12–14 g body weight) were infected intraperitoneally with JEV[2]
    Dosage: 50, 75 or 100 mg/kg/day
    Administration: Administered intragastrically by gavage
    Result: 50 mg/kg/day, 75 mg/kg/day and 100 mg/kg/day led to 30%, 70% and 90% mice survival, respectively.
    Molecular Weight

    307.28

    Formula

    C12H9N3O5S
    CAS No.
    Appearance

    Solid

    Color

    Light yellow to yellow

    SMILES

    O=C(NC1=NC=C([N+]([O-])=O)S1)C2=CC=CC=C2OC(C)=O
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage
    Powder -20°C 3 years
    4°C 2 years
    In solvent -80°C 2 years
    -20°C 1 year
    Solvent & Solubility
    In Vitro: 

    DMSO : ≥ 100 mg/mL (325.44 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    *"≥" means soluble, but saturation unknown.

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 3.2544 mL 16.2718 mL 32.5436 mL
    5 mM 0.6509 mL 3.2544 mL 6.5087 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

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    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
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    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

    C1

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    Volume (start)

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    C2

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    In Vivo:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 3.25 mg/mL (10.58 mM); Clear solution

      This protocol yields a clear solution of ≥ 3.25 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (32.5 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

      Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Please enter your animal formula composition:
    %
    DMSO +
    % 
    +
    %
    Tween-80 +
    %
    Saline
    Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
    The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
    Calculation results:
    Working solution concentration: mg/mL
    Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
    Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
     If the continuous dosing period exceeds half a month, please choose this protocol carefully.
    Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
    Purity & Documentation

    Purity: 99.95%

    COA (253 KB) RP-HPLC (253 KB)

    Handling Instructions (2659 KB)
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 3.2544 mL 16.2718 mL 32.5436 mL 81.3590 mL
    5 mM 0.6509 mL 3.2544 mL 6.5087 mL 16.2718 mL
    10 mM 0.3254 mL 1.6272 mL 3.2544 mL 8.1359 mL
    15 mM 0.2170 mL 1.0848 mL 2.1696 mL 5.4239 mL
    20 mM 0.1627 mL 0.8136 mL 1.6272 mL 4.0680 mL
    25 mM 0.1302 mL 0.6509 mL 1.3017 mL 3.2544 mL
    30 mM 0.1085 mL 0.5424 mL 1.0848 mL 2.7120 mL
    40 mM 0.0814 mL 0.4068 mL 0.8136 mL 2.0340 mL
    50 mM 0.0651 mL 0.3254 mL 0.6509 mL 1.6272 mL
    60 mM 0.0542 mL 0.2712 mL 0.5424 mL 1.3560 mL
    80 mM 0.0407 mL 0.2034 mL 0.4068 mL 1.0170 mL
    100 mM 0.0325 mL 0.1627 mL 0.3254 mL 0.8136 mL
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    Nitazoxanide Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Nitazoxanide55981-09-4NTZ NSC 697855NSC697855NSC 697855NSC-697855ParasiteInfluenza VirusAutophagyanthelmintichelminthsprotozoaentericbacteria infectingGiardia lambliatrophozoiteparasiticgastroenteritis ,virusJEVInhibitorinhibitorinhibit

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