Nitidine chloride

Name: Nitidine chloride
Brand: MedChemExpress
SKU: HY-N0498
Rating: 5.0 (3 reviews)

Cat. No.: HY-N0498 Purity: 99.75%: FAQs
Data Sheet SDS COA Handling Instructions

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Nitidine chloride, a potential anti-malarial lead compound derived from Zanthoxylum nitidum (Roxb) DC, exerts potent anticancer activity through diverse pathways, including inducing apoptosis, inhibiting STAT3 signaling cascade, DNA topoisomerase 1 and 2A, ERK and c-Src/FAK associated signaling pathway, also has anti-inflammatory activity. Nitidine chloride inhibits LPS-induced inflammatory cytokines production via MAPK and NF-kB pathway.

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Nitidine chloride Chemical Structure

CAS No. : 13063-04-2

Size	Price	Stock	Quantity
5 mg	USD 60	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 100	In-stock	Estimated Time of Arrival: December 31
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Based on 3 publication(s) in Google Scholar

Other Forms of Nitidine chloride:

Nitidine chloride (Standard) Get quote

3 Publications Citing Use of MCE Nitidine chloride

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Amebae Coccidia Leishmania Mite Plasmodium Toxoplasma Trypanosoma Schistosome

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STAT3 STAT5 STAT6 STAT1

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Topoisomerase Topo I Topo II

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ERK ERK1 ERK2 ERK5 ERK8

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p38 MAPK p38α p38β MAPK13 p38δ p38γ

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NF-κB NF-κB1/p50 RelA/p65 RelB c-Rel

Biological Activity
Purity & Documentation
References
Customer Review

Description

IC₅₀ & Target

Plasmodium

Cellular Effect

Cell Line	Type	Value	Description	References
HCT-116	IC₅₀	4.42 μM Compound: 1c	Antiproliferative activity against human HCT116 cells assessed as inhibition of cell viability after 48 hrs by MTT assay Antiproliferative activity against human HCT116 cells assessed as inhibition of cell viability after 48 hrs by MTT assay	[PMID: 26026362]
HeLa	IC₅₀	0.05 μg/mL Compound: 1	Growth inhibition of human HeLa S3 cells after 72 hrs Growth inhibition of human HeLa S3 cells after 72 hrs	[PMID: 10395504]
HL-60	IC₅₀	3.41 μM Compound: 1c	Antiproliferative activity against human HL60 cells assessed as inhibition of cell viability after 48 hrs by WST-1 assay Antiproliferative activity against human HL60 cells assessed as inhibition of cell viability after 48 hrs by WST-1 assay	[PMID: 26026362]
HT-29	IC₅₀	2.75 μM Compound: 1, 1-k6932	Cytotoxicity against human HT-29 cells after 72 hrs by MTS assay Cytotoxicity against human HT-29 cells after 72 hrs by MTS assay	[PMID: 21435753]

In Vitro

Nitidine chloride (0-32 μM, 24-48 h) inhibits the proliferation of A498 and 768-O cells^[1].
Nitidine chloride (8-16 μM, 24 h) induces apoptosis in A498 and 768-O cells^[1].
Nitidine chloride (0-16 μM, 48 h) induces apoptosis in A498 cells, causes cell cycle arrest, and inhibits the ERK pathway^[1].
Nitidine chloride (0-10 μM, 24 h) induces apoptosis in HSC3 and HSC4 cells, inducing apoptosis in OSCC cells through dephosphorylation of STAT3^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[1]

Cell Line:	768-O, A498
Concentration:	0, 4, 6, 8, 16, 32 μM
Incubation Time:	24, 48 h
Result:	Inhibited cell proliferation in a time- and dose-dependent manner.

Apoptosis Analysis^[1]

Cell Line:	768-O, A498
Concentration:	8, 16 μM
Incubation Time:	24 h
Result:	Increased apoptosis rate in a dose-dependent manner.

Western Blot Analysis^[1]

Cell Line:	A498
Concentration:	0, 8, 16 μM
Incubation Time:	48 h
Result:	Increased Bax expression, decreased Bcl-2 expression, increased p53 expression, decreased pro-caspase-3 expression, increased cleaved caspase-3 expression and PARP cleavage level, inhibited ERK1/2, p38 and Akt phosphorylation.

Cell Viability Assay^[2]

Cell Line:	HSC3, HSC4
Concentration:	0, 0.1, 5, 10 μM
Incubation Time:	24 h
Result:	Reduced cell viability.

Western Blot Analysis^[2]

Cell Line:	HSC3, HSC4, OSCC
Concentration:	0, 0.1, 5, 10 μM
Incubation Time:	24 h
Result:	Dephosphorylated STAT3 and induced the cleavage of PARP and caspase-3 in a concentration- and time-dependent manner.

In Vivo

Nitidine chloride (5 mg/kg, intraperitoneal injection, once daily, 5 weeks) inhibits tumor growth in mice and induces apoptosis in renal cancer cells in vivo^[1].
Nitidine chloride (0.375-10 mg/kg, intraperitoneal injection, once daily, 3 weeks) downregulates hypertrophy in mouse myocardium through AGT4B-mediated autophagy^[3].
Nitidine chloride (5-10 mg/kg, intraperitoneal injection, single dose) exerts anti-inflammatory effects in endotoxin-treated mice through Akt-mediated IL-10 production^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	A549 nude mouse xenograft model^[1]
Dosage:	5 mg/kg; daily; 5 weeks
Administration:	Intraperitoneal injection (i.p.)
Result:	Showed non-toxic effects, reduced tumor growth, and induced apoptosis in renal cancer cells in vivo.

Animal Model:	Mice, beagles^[3]
Dosage:	2.5, 5, 10 mg/kg; 0.375, 0.75, 1.5 mg/kg; daily; 4 weeks
Administration:	Intraperitoneal injection (i.p.)
Result:	Both induced dose-dependent cardiac hypertrophy and dysfunction, while medium and high doses led to death in beagle dogs. Cardiac autophagy levels in cardiomyocytes were reduced. Overexpression of ATG4B reversed cardiac hypertrophy and reduced autophagy levels.

Animal Model:	LPS induced endotoxemic mice^[4]
Dosage:	5, 10 mg/kg; single dose
Administration:	Intraperitoneal injection (i.p.)
Result:	Increased IL-10 production, inhibited inflammatory response, reduced mortality, and enhanced Akt activation.

Molecular Weight

383.82

Formula

C₂₁H₁₈ClNO₄

CAS No.

13063-04-2

Appearance

Solid

Color

Light yellow to yellow

SMILES

C[N+]1=CC2=CC(OC)=C(OC)C=C2C(C=CC3=C4)=C1C3=CC5=C4OCO5.[Cl-]

Structure Classification

Initial Source

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvent & Solubility

In Vitro:

DMSO : 2 mg/mL (5.21 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

*Nitidine chloride is usually formulated as a suspension.

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.6054 mL	13.0269 mL	26.0539 mL
5 mM	0.5211 mL	2.6054 mL	5.2108 mL
10 mM	---	---	---

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 0.5% CMC-Na/0.5% Tween-80 in Saline water
Solubility: 1 mg/mL (2.61 mM); Suspended solution; Need ultrasonic
Protocol 2

Add each solvent one by one: 15% Cremophor EL 85% Saline
Solubility: 2 mg/mL (5.21 mM); Suspended solution; Need ultrasonic

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

Purity & Documentation

Purity: 99.75%

Select Batch:

Data Sheet (284 KB) SDS (420 KB)

COA (251 KB) RP-HPLC (244 KB) MS (68 KB)

Handling Instructions (2659 KB)

References

[1]. Fang Z, et al. Nitidine chloride induces apoptosis and inhibits tumor cell proliferation via suppressing ERK signaling pathway in renal cancer. Food Chem Toxicol. 2014 Apr;66:210-6. [Content Brief]

[2]. Lee-Han Kim, et al. Nitidine chloride acts as an apoptosis inducer in human oral cancer cells and a nude mouse xenograft model via inhibition of STAT3. Oncotarget. 2017 Aug 24;8(53):91306-91315. [Content Brief]

[3]. Yang Hong, et al. Nitidine chloride induces cardiac hypertrophy in mice by targeting autophagy-related 4B cysteine peptidase. Acta Pharmacol Sin. 2023 Mar;44(3):561-572. [Content Brief]

[4]. Niao Yang, et al. Nitidine chloride exerts anti-inflammatory action by targeting Topoisomerase I and enhancing IL-10 production. Pharmacol Res. 2019 Oct:148:104368. [Content Brief]

[5]. Wang Z, et al. Nitidine chloride inhibits LPS-induced inflammatory cytokines production via MAPK and NF-kappaB pathway in RAW 264.7 cells. J Ethnopharmacol. 2012 Oct 31;144(1):145-50. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.6054 mL	13.0269 mL	26.0539 mL	65.1347 mL
DMSO	5 mM	0.5211 mL	2.6054 mL	5.2108 mL	13.0269 mL