Organoid Culture

Recombinant Protein Expression Service

An organoid is a 3D multicellular in vitro tissue construct that mimics the complex structure and functionality of corresponding in vivo organ. Organoids can be generated from stem cells in adult tissue or from pluripotent stem cells. Organoid structures provide a very useful tool as a model to study tissue development and disease, personalized medicine, drug screening and cell therapy
Organoids recapitulate multiple biological parameters including the spatial organization of heterogeneous tissue-specific cells, cell-cell interactions, and cell-matrix interactions, and certain physiological functions generated by tissue-specific cells within the organoid. They are more physiologically relevant than monolayer culture models and are far more amenable to manipulation of niche components, signalling pathways and genome editing than in vivo models.
Organoids can be fast expanded long-term, cryopreserved and applied to high-throughput analyses.

Organoids are generated from tissue-specific adult stem cells or ESCs/iPSCs. Organoids are typically cultured in an extracellular matrix (ECM) surrounded by culture media supplemented with niche factors specific to the organoid type. Common niche and ECM factors: R-spondin (RSPO1), EGF, Noggin, Activin A, Collagen.

Common Reagents for Organoid Culture

Recombinant Proteins

Cat. No	Product Name	Proteins	Function
HY-P70453C HY-P70453A	Human Wnt3a Surrogate Human Wnt3a	Wnt	An essential niche component for maintaining the proliferation of Lgr5-positive stem cells in various organoids, such as the intestinal, gastric, pancreatic and liver organoids.
HY-P7109 HY-P70590	Human EGF Mouse EGF	EGF	A growth factor for epithelial tissues; binding to EGF receptors, induces hyperplasic changes. Used for the generation of intestinal, liver, thyroid, and brain organoids.
HY-P7051A HY-P7086	Human Noggin Mouse Noggin	Noggin	An inhibitor of bone morphogenetic proteins that modulates cellular differentiation, proliferation, and apoptosis.
HY-P7114 HY-P76012	Human R-spondin-1 Mouse R-spondin-1	R-spondin	The ligand of Lgr5 and a niche factor that is required for the self-renewal of stem cells and activates Wnt signaling. An essential additive of the organoid culture system.
HY-P7014 HY-P7047A HY-P7177 HY-P70695 HY-P7172 HY-P7004	Human FGF-4 Human FGF-7 Human FGF-9 Human FGF-10 Human FGF-19 Human FGF-basic/FGF-2	FGF	FGFs play crucial roles in a wide variety of cellular functions, including cell proliferation, survival, metabolism, morphogenesis, and differentiation, as well as in tissue repair and regeneration. In a 3D extracellular matrix, FGF-2, FGF-7, FGF-9, and FGF-10 promote lung organoid formation.
HY-P7007 HY-P7008 HY-P7006	Human BMP-4 Human BMP-7 Human/Mouse/Rat BMP-2	BMP	BMPs play crucial roles in embryogenesis and development, and also in maintenance of adult tissue homeostasis. BMP-2 and BMP-4 are widely used in in vitro generation of hepatic cells from iPSCs and ESCs.
HY-P7420 HY-P7312	Human VEGF-A Mouse VEGF-A	VEGF	VEGF-A is required during embryogenesis to regulate the proliferation, migration, and survival of endothelial cells. It is used in the generation of vascular organoids.
HY-P70699	Mouse PDGF-BB	PDGF	PDGF-BB induces vascular smooth muscle cells (VSMC) specification and cell differentiation in the vascular.
HY-P7121	Human HGF	HGF	A known hepatocyte mitogen that can be used for the liver organoid culture.
HY-P70311	Human/Mouse/Rat Activin A	Activin A	A cytokine with multiple roles in development and homeostasis.In the case of intestinal organoids, it activates TGF-β signaling in PSCs to trigger endodermal differentiation.
HY-P7155A	Human DKK-1	DKK	A canonical WNT inhibitor that can induce retinal progenitors for self-organization.

Basement Membrane Matrix

Cat. No	Product Name	Application
HY-K6001	Basement Membrane Matrix (Phenol Red)	In vitro angiogenesis, tumor cell migration or invasion
HY-K6002	Basement Membrane Matrix	In vitro angiogenesis, tumor cell migration or invasion
HY-K6003	Basement Membrane Matrix GFR (Phenol Red)	Organoid culture, in vitro angiogenesis
HY-K6004	Basement Membrane Matrix GFR	Organoid culture, in vitro angiogenesis
HY-K6005	Basement Membrane Matrix HC (Phenol Red)	Transplantation/induction of tumorigenic models such as PDX, CDX
HY-K6006	Basement Membrane Matrix IPSC-qualified	Stem cell expansion and differentiation
HY-K6007	Basement Membrane Matrix for Organoid Culture	Organoid culture

Organoid culture kit

organoid medium	Cat. No	Types of tissue	Cat. No	Types of tissue
Tumor organoid medium	HY-K6101	Human breast cancer	HY-K6106	Human Cholangiocarcinoma
	HY-K6102	Human Lung Adenocarcinoma	HY-K6107	Human Cervical Cancer
	HY-K6103	Human Small Cell Lung Cancer	HY-K6108	Human Esophageal Cancer
	HY-K6104	Human Colorectal Cancer	HY-K6109	Human Endometrial Cancer
	HY-K6105	Human Gastric Cancer	HY-K6110	Human Pancreatic Cancer
	HY-K6111	Human Head and Neck Squamous Cell Carcinoma	HY-K6120	Mouse Colonic
Normal Tissue organoid Medium	HY-K6112	Human Colonic	HY-K6116	Human Kidney Tubular
	HY-K6113	Human Intestinal	HY-K6117	Human Liver Ductal
	HY-K6114	Human Gastric Epithelial	HY-K6118	Mouse Liver Ductal
	HY-K6115	Human Pancreatic	HY-K6119	Mouse Intestinal

Experiment validation

Measured by its ability to induce Topflash reporter activity in HEK293T human embryonic kidney cells. The ED₅₀ for this effect is 5.2 ng/mL. The ED₅₀ of human Wnt Surrogate from MedChemExpress's each Lot is lower than of Competitor P. The ED₅₀ is <0.2 ng/mL as measured by murine BALB/c 3T3 cells. Measured by its ability to inhbit BMP-4-induced alkalnephosphatase production by ATDC5 mouse chondrogenic cells in the presence of Recombinant Human BMP-4 (40 ng/mL), with an ED₅₀ of 4 ng/mL.

HY-K6002
Mouse liver ductal organoids
cultured by MedChemExpress (MCE) Basement
Membrane Matrix

HY-K6003
Mouse trachea organoids
cultured by MedChemExpress (MCE) Basement
Membrane Matrix GFR
(Phenol Red)

HY-K6006
Mouse intestinal organoid
cultured by MedChemExpress (MCE) Basement
Membrane Matrix
IPSC-qualified

D11

HY-K6005
Tumor volume in tumor cell-loaded tumor
model (CDX) mice by MedChemExpress (MCE) Basement
Membrane Matrix HC (Phenol Red)

Name
Email *

	Sorry, but the email address you supplied was invalid.