Polyphyllin I

Name: Polyphyllin I
Brand: MedChemExpress
SKU: HY-N0047
Rating: 5.0 (2 reviews)

Cat. No.: HY-N0047 Purity: 99.89%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

Polyphyllin I is a bioactive constituent extracted from Paris polyphylla, has strong anti-tumor activity. Polyphyllin I is an activator of the JNK signaling pathway and is an inhibitor of PDK1/Akt/mTOR signaling. Polyphyllin I induces autophagy, G2/M phase arrest and apoptosis.

For research use only. We do not sell to patients.

Polyphyllin I Chemical Structure

CAS No. : 50773-41-6

Size	Price	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 103	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 103	In-stock	Estimated Time of Arrival: December 31
Solid
5 mg	USD 66	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 110	In-stock	Estimated Time of Arrival: December 31
20 mg	USD 198	In-stock	Estimated Time of Arrival: December 31
50 mg		Get quote
100 mg		Get quote

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Customer Review

Based on 2 publication(s) in Google Scholar

Other Forms of Polyphyllin I:

Polyphyllin I (Standard) Get quote

1 Publications Citing Use of MCE Polyphyllin I

•FASEB J. 2020 Dec;34(12):16414-16431. [Abstract]

: Polyphyllin I purchased from MedChemExpress. Usage Cited in: FASEB J. 2020 Dec;34(12):16414-16431. [Abstract]; HEK293-T cells are co-transfected with pFUW vector and tau plasmids, or pFUW-CIP2A and tau plasmids, with or without Polyphyllin I (PPI; 0.5 or 1.0 μM) incubation for 48 h.

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Biological Activity
Purity & Documentation
References
Customer Review

Description

IC₅₀ & Target

JNK signaling^[2]
PDK1/Akt/mTOR signaling^[3]

Cellular Effect

Cell Line	Type	Value	Description	References
Bel-7402	IC₅₀	4.74 μM Compound: PPI	Cytotoxicity against human Bel7402 cells after 24 hrs by MTT assay Cytotoxicity against human Bel7402 cells after 24 hrs by MTT assay	[PMID: 27623551]
HepG2	IC₅₀	4.01 μM Compound: PPI	Cytotoxicity against human HepG2 cells after 24 hrs by MTT assay Cytotoxicity against human HepG2 cells after 24 hrs by MTT assay	[PMID: 27623551]
NCI-H1299	IC₅₀	< 1 μM Compound: PSI	Cytotoxicity against human NCI-H1299 cells assessed as reduction in cell viability after 48 hrs by MTT assay Cytotoxicity against human NCI-H1299 cells assessed as reduction in cell viability after 48 hrs by MTT assay	[PMID: 27721159]
NCI-H446	IC₅₀	< 1 μM Compound: PSI	Cytotoxicity against human NCI-H446 cells assessed as reduction in cell viability after 48 hrs by MTT assay Cytotoxicity against human NCI-H446 cells assessed as reduction in cell viability after 48 hrs by MTT assay	[PMID: 27721159]
NCI-H460	IC₅₀	< 1 μM Compound: PSI	Cytotoxicity against human NCI-H460 cells assessed as reduction in cell viability after 48 hrs by MTT assay Cytotoxicity against human NCI-H460 cells assessed as reduction in cell viability after 48 hrs by MTT assay	[PMID: 27721159]
NCI-H520	IC₅₀	< 1 μM Compound: PSI	Cytotoxicity against human NCI-H520 cells assessed as reduction in cell viability after 48 hrs by MTT assay Cytotoxicity against human NCI-H520 cells assessed as reduction in cell viability after 48 hrs by MTT assay	[PMID: 27721159]

In Vitro

Polyphyllin I (0.625-10 mg/mL; 24-72 h) inhibits the proliferation of three non-small cell lung cancer (NSCLC) cell lines, with the IC₅₀s of 1.24, 2.40, and 2.33 μg/ml for A549, H460, and SK-MES-1 cells, respectively^[1].
Polyphyllin I (2.5 mg/mL; 6-24 h) induces apoptosis of A549 cells^[1].
Polyphyllin I (0.25-2 μM; 24 h) increase in LC3-II expression and decrease in P62 expression in HGC-27 cells^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Proliferation Assay^[1]

Cell Line:	A549, H460, and SK-MES-1 cells^[1]
Concentration:	24, 48, 72 hours
Incubation Time:	24, 48, 72 hours
Result:	Markedly reduced proliferation capacity in vitro and in a concentration-dependent manner.

In Vivo

Polyphyllin I (1.5 mg/kg; i.p. twice daily from day 2 to day 11) significantly inhibit the tumor growth of A549 cells in the nude mice^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	BALB/c nude mice (5 weeks old) were s.c. inoculated with A549 cells^[1]
Dosage:	1.5 mg/kg
Administration:	I.p. twice daily from day 2 to day 11
Result:	The tumor growth rate was much lower than that in cisplatin group and PBS group.

Molecular Weight

855.02

Formula

C₄₄H₇₀O₁₆

CAS No.

50773-41-6

Appearance

Solid

Color

White to off-white

SMILES

C[C@H](CC1)CO[C@]21O[C@@]3([H])C[C@@]4([H])[C@@]5([H])[C@]([C@](CC[C@H](O[C@]6([H])O[C@H](CO)[C@@H](O[C@]7([H])O[C@@H](CO)[C@H](O)[C@H]7O)[C@H](O)[C@H]6O[C@@]8([H])[C@H](O)[C@H](O)[C@@H](O)[C@H](C)O8)C9)(C)C9=CC5)([H])CC[C@]4(C)[C@@]3([H])[C@@H]2C

Structure Classification

Saccharides

Steroids

Initial Source

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (116.96 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : < 0.1 mg/mL (insoluble)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.1696 mL	5.8478 mL	11.6956 mL
5 mM	0.2339 mL	1.1696 mL	2.3391 mL
10 mM	0.1170 mL	0.5848 mL	1.1696 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (2.92 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 2

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (2.92 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.
Protocol 3

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 0.83 mg/mL (0.97 mM); Clear solution

This protocol yields a clear solution of ≥ 0.83 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (8.3 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.89%

Select Batch:

Data Sheet (279 KB) SDS (251 KB)

COA (255 KB) HNMR (351 KB) RP-HPLC (239 KB) MS (68 KB)

Handling Instructions (2659 KB)

References

[1]. Kong M, et al. Effects of polyphyllin I on growth inhibition of human non-small lung cancer cells and in xenograft. Acta Biochim Biophys Sin (Shanghai). 2010 Nov;42(11):827-33. [Content Brief]

[2]. Liu J, et al. Polyphyllin I induces G2/M phase arrest and apoptosis in U251 human glioma cells via mitochondrial dysfunction and the JNK signaling pathway. Acta Biochim Biophys Sin (Shanghai). 2017 Jun 1;49(6):479-486. [Content Brief]

[3]. He J, et al. Polyphyllin I induces autophagy and cell cycle arrest via inhibiting PDK1/Akt/mTOR signal and downregulating cyclin B1 in human gastric carcinoma HGC-27 cells. Biomed Pharmacother. 2019 Sep;117:109189. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	1.1696 mL	5.8478 mL	11.6956 mL	29.2391 mL
	5 mM	0.2339 mL	1.1696 mL	2.3391 mL	5.8478 mL
	10 mM	0.1170 mL	0.5848 mL	1.1696 mL	2.9239 mL
	15 mM	0.0780 mL	0.3899 mL	0.7797 mL	1.9493 mL
	20 mM	0.0585 mL	0.2924 mL	0.5848 mL	1.4620 mL
	25 mM	0.0468 mL	0.2339 mL	0.4678 mL	1.1696 mL
	30 mM	0.0390 mL	0.1949 mL	0.3899 mL	0.9746 mL
	40 mM	0.0292 mL	0.1462 mL	0.2924 mL	0.7310 mL
	50 mM	0.0234 mL	0.1170 mL	0.2339 mL	0.5848 mL
	60 mM	0.0195 mL	0.0975 mL	0.1949 mL	0.4873 mL
	80 mM	0.0146 mL	0.0731 mL	0.1462 mL	0.3655 mL
	100 mM	0.0117 mL	0.0585 mL	0.1170 mL	0.2924 mL

Polyphyllin I Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Polyphyllin I

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Other Forms of Polyphyllin I:

Polyphyllin I Related Antibodies

1 Publications Citing Use of MCE Polyphyllin I

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

View All JNK Isoform Specific Products:

View All mTOR Isoform Specific Products:

View All Akt Isoform Specific Products:

Biological Activity

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References

Customer Review

Polyphyllin I Related Antibodies

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Dilution Calculator

Complete Stock Solution Preparation Table

Polyphyllin I Related Classifications

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