2. Metabolic Enzyme/Protease Apoptosis
  3. Aldehyde Dehydrogenase (ALDH) Apoptosis
  4. Alda-1

Alda-1 is a potent and selective ALDH2 agonist, which activates wild-type ALDH2 and restores near wild-type activity to ALDH2*2.

For research use only. We do not sell to patients.

Alda-1 Chemical Structure

Alda-1 Chemical Structure

CAS No. : 349438-38-6

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Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 79 In-stock
Solution
10 mM * 1 mL in DMSO USD 79 In-stock
Solid
5 mg USD 46 In-stock
10 mg USD 72 In-stock
50 mg USD 116 In-stock
100 mg USD 209 In-stock
500 mg USD 627 In-stock
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Customer Review

Based on 18 publication(s) in Google Scholar

Alda-1 Related Antibodies

Top Publications Citing Use of Products

    Alda-1 purchased from MedChemExpress. Usage Cited in: Life Sci. 2018 Dec 15;215:227-235.  [Abstract]

    VEGF-α representative western blot and relative density of immunoblot in DMSO group and Alda1-treated group at 1 d, 7 d and 30 d post aged diabetic rats model established.

    Alda-1 purchased from MedChemExpress. Usage Cited in: J Immunol Res. 2018 Dec 24;2018:9807139.  [Abstract]

    Western blot assays show that 3-MA abrogates the Alda-1 induced expression increases in LC3BⅡ and Bcl2 and decreases in P62 and Bax.

    Alda-1 purchased from MedChemExpress. Usage Cited in: J Immunol Res. 2018 Dec 24;2018:9807139.  [Abstract]

    Western blot analysis of p-AMPK, AMPK, Bcl2 and Bax, LC3B and P62 expression in liver tissues.

    View All Aldehyde Dehydrogenase (ALDH) Isoform Specific Products:

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    ALDH1 ALDH2 ALDH3

    • Biological Activity

    • Protocol

    • Purity & Documentation

    • References

    • Customer Review

    Description

    Alda-1 is a potent and selective ALDH2 agonist, which activates wild-type ALDH2 and restores near wild-type activity to ALDH2*2.

    IC50 & Target

    ALDH2

     

    In Vivo

    Alda-1 treatment results in a significant decrease of 4-HNE-protein content in the plasma of apoE−/− mice. Alda-1 administration leads to a slight increase in gene expression related to neurogenesis (Nog), mitochondrial biogenesis (CYTB, ND1), and apoptosis (Bax, Gsk3b) in the Hp of apoE−/− mice. Alda-1 administration leads to 2 and 10 differentially expressed proteins in the FCx and Hp of apoE−/− mice, respectively[1].
    Alda-1 (1.5 mg/kg, b.w., IP) administration significantly increases the climbing time, tends to reduce the immobility time and increases the swimming time of the prenatally stressed rats in the forced swim test. Moreover, treatment of prenatally stressed rats with Alda-1 significantly increases number of entries into the open arms of the maze and the time spent therein, as assessed by elevated plus-maze test[2]. Alda-1 (8.5 mg/kg; IP) with glucose significantly lowers 4-HNE and FJB-positive cells in the cerebral cortex of Alda-1-treated rats than in DMSO-treated rats 24 h after glucose administration[3].
    Alda-1 (10 mg/kg per day) treatment prevents aldehydic overload, mitochondrial dysfunction and improves ventricular function in post-MI cardiomyopathy rats[4].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
    Molecular Weight

    324.16

    Formula

    C15H11Cl2NO3
    CAS No.
    Appearance

    Solid

    Color

    White to off-white

    SMILES

    O=C(NCC1=CC=C(OCO2)C2=C1)C3=C(Cl)C=CC=C3Cl
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage
    Powder -20°C 3 years
    4°C 2 years
    In solvent -80°C 6 months
    -20°C 1 month
    Solvent & Solubility
    In Vitro: 

    DMSO : ≥ 51 mg/mL (157.33 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    *"≥" means soluble, but saturation unknown.

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 3.0849 mL 15.4245 mL 30.8490 mL
    5 mM 0.6170 mL 3.0849 mL 6.1698 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
    =
    Concentration
    ×
    Volume
    ×
    Molecular Weight *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

    C1

    ×
    Volume (start)

    V1

    =
    Concentration (final)

    C2

    ×
    Volume (final)

    V2

    In Vivo:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    90% Corn Oil

      Solubility: ≥ 2.5 mg/mL (7.71 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

    For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  0.5% CMC-Na/saline water

      Solubility: 24 mg/mL (74.04 mM); Suspended solution; Need ultrasonic

    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Please enter your animal formula composition:
    %
    DMSO +
    % 
    +
    %
    Tween-80 +
    %
    Saline
    Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
    The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
    Calculation results:
    Working solution concentration: mg/mL
    Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
    Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
     If the continuous dosing period exceeds half a month, please choose this protocol carefully.
    Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
    Purity & Documentation

    Purity: 99.99%

    COA (249 KB) LCMS (108 KB)

    Handling Instructions (2659 KB)
    References
    Cell Assay
    [2]

    Spleen cells (4×106 cells/mL) are stimulated by optimal concentrations of concanavalin A (Con A; 2.5 μg/mL and 0.6 μg/mL) and lipopolysaccharide (LPS, 5 μg/mL) and are incubated in 96-well plates at final volume of 0.2 mL for 72 h. Cell proliferation is determined by adding 0.5 μCi of [3H]-thymidine per well at 16 h before the end of the incubation. The cultures are harvested with an automatic cell harvester, and [3H] thymidine incorporation is assessed using a liquid scintillation counter.

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.
    Animal Administration
    [2]

    After behavioral verification at three months of age, the animals are divided into the following four groups: control, control + Alda-1, prenatally stressed and prenatally stressed + Alda-1 (6 animals per group). Alda-1 injections are given intraperitoneally (i.p.) once daily at a dose of 1.5 mg/kg b.w. (dissolved in 1 mL/kg b.w. DMSO/water 50/50) for 14 days. At the same time, the control and prenatally stressed rats receive 1 mL/kg b.w. DMSO/water 50/50. The injections of Alda-1 and vehicle are given between 10 a.m and 11 a.m. In the last five days of Alda-1 treatment the behavioral parameters in the elevated plus maze test and then in the forced swim test are measured.

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 3.0849 mL 15.4245 mL 30.8490 mL 77.1224 mL
    5 mM 0.6170 mL 3.0849 mL 6.1698 mL 15.4245 mL
    10 mM 0.3085 mL 1.5424 mL 3.0849 mL 7.7122 mL
    15 mM 0.2057 mL 1.0283 mL 2.0566 mL 5.1415 mL
    20 mM 0.1542 mL 0.7712 mL 1.5424 mL 3.8561 mL
    25 mM 0.1234 mL 0.6170 mL 1.2340 mL 3.0849 mL
    30 mM 0.1028 mL 0.5141 mL 1.0283 mL 2.5707 mL
    40 mM 0.0771 mL 0.3856 mL 0.7712 mL 1.9281 mL
    50 mM 0.0617 mL 0.3085 mL 0.6170 mL 1.5424 mL
    60 mM 0.0514 mL 0.2571 mL 0.5141 mL 1.2854 mL
    80 mM 0.0386 mL 0.1928 mL 0.3856 mL 0.9640 mL
    100 mM 0.0308 mL 0.1542 mL 0.3085 mL 0.7712 mL
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    Alda-1 Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Alda-1349438-38-6Alda1Alda 1Aldehyde Dehydrogenase (ALDH)ApoptosisInhibitorinhibitorinhibit

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