BRD4 degrader AT1

Name: BRD4 degrader AT1
Brand: MedChemExpress
SKU: HY-111433
Rating: 4.5 (1 reviews)

Cat. No.: HY-111433 Purity: 99.09%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

BRD4 degrader AT1 is a PROTAC connected by ligands for von Hippel-Lindau and BRD4 as a highly selective Brd4 degrader, with a K_d of 44 nM for Brd4^BD2 in cells.

For research use only. We do not sell to patients.

BRD4 degrader AT1 Chemical Structure

CAS No. : 2098836-45-2

Size	Price	Stock	Quantity
1 mg	USD 161	In-stock	Estimated Time of Arrival: December 31
5 mg	USD 340	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 590	In-stock	Estimated Time of Arrival: December 31
25 mg	USD 1200	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 2100	In-stock	Estimated Time of Arrival: December 31
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200 mg		Get quote

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Customer Review

Based on 1 publication(s) in Google Scholar

1 Publications Citing Use of MCE BRD4 degrader AT1

•Structure. 2024 Sep 28:S0969-2126(24)00385-X. [Abstract]

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von Hippel-Lindau (VHL) Cereblon MDM2 cIAP1

Biological Activity
Purity & Documentation
References
Customer Review

Description

BRD4 degrader AT1 is a PROTAC connected by ligands for von Hippel-Lindau and BRD4 as a highly selective Brd4 degrader, with a K_d of 44 nM for Brd4^BD2 in cells.

IC₅₀ & Target

Kd: 44 nM (Brd4^BD2, cell assay), 38.8 nM (Brd4^BD2 QVK, cell assay), 111 nM (Brd2^BD1, cell assay), 94 nM (Brd2^BD2, cell assay), 35 nM (Brd3^BD1, cell assay), 39 nM (Brd3^BD2, cell assay), 75 nM (Brd4^BD1, cell assay), 35 nM (Brd2^BD1 KEA, cell assay)^[1]

In Vitro

BRD4 degrader AT1 is a highly selective Brd4 degrader, with a K_d of 44 nM, for Brd4^BD2 in cells. BRD4 degrader AT1 also shows K_ds of 38.8 nM for a mutant Brd4^BD2 (QVK), 111 ± 14 nM for Brd2^BD1, 94 ± 9 nM for Brd2^BD2, 35 ± 3 nM for Brd3^BD1, 39 ± 8 nM for Brd3^BD2, 75 ± 23 nM for Brd4^BD1 and 35 ± 4 nM for a Brd2^BD1 mutant (KEA). BRD4 degrader AT1 (1-3 μM) causes remarkable Brd4-selective depletion and has no or little activity against Brd2 and Brd3^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

972.68

Formula

C₄₈H₅₈ClN₉O₅S₃

CAS No.

2098836-45-2

Appearance

Solid

Color

Off-white to light yellow

SMILES

CC1=C(C)SC2=C1C(C3=CC=C(Cl)C=C3)=N[C@@H](CC(NCCCCCCSC(C)(C)[C@H](NC(C)=O)C(N4[C@@H](C[C@@H](O)C4)C(NCC5=CC=C(C6=C(C)N=CS6)C=C5)=O)=O)=O)C7=NN=C(C)N27

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (102.81 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.0281 mL	5.1404 mL	10.2809 mL
5 mM	0.2056 mL	1.0281 mL	2.0562 mL
10 mM	0.1028 mL	0.5140 mL	1.0281 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 1.17 mg/mL (1.20 mM); Clear solution

This protocol yields a clear solution of ≥ 1.17 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (11.7 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 1.17 mg/mL (1.20 mM); Clear solution

This protocol yields a clear solution of ≥ 1.17 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (11.7 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 1.17 mg/mL (1.20 mM); Clear solution

This protocol yields a clear solution of ≥ 1.17 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (11.7 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.09%

Select Batch:

Data Sheet (270 KB) SDS (393 KB)

COA (245 KB) RP-HPLC (244 KB)

Handling Instructions (2659 KB)

References

[1]. Gadd MS, et al. Structural basis of PROTAC cooperative recognition for selective protein degradation. Nat Chem Biol. 2017 May;13(5):514-521. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	1.0281 mL	5.1404 mL	10.2809 mL	25.7022 mL
	5 mM	0.2056 mL	1.0281 mL	2.0562 mL	5.1404 mL
	10 mM	0.1028 mL	0.5140 mL	1.0281 mL	2.5702 mL
	15 mM	0.0685 mL	0.3427 mL	0.6854 mL	1.7135 mL
	20 mM	0.0514 mL	0.2570 mL	0.5140 mL	1.2851 mL
	25 mM	0.0411 mL	0.2056 mL	0.4112 mL	1.0281 mL
	30 mM	0.0343 mL	0.1713 mL	0.3427 mL	0.8567 mL
	40 mM	0.0257 mL	0.1285 mL	0.2570 mL	0.6426 mL
	50 mM	0.0206 mL	0.1028 mL	0.2056 mL	0.5140 mL
	60 mM	0.0171 mL	0.0857 mL	0.1713 mL	0.4284 mL
	80 mM	0.0129 mL	0.0643 mL	0.1285 mL	0.3213 mL
	100 mM	0.0103 mL	0.0514 mL	0.1028 mL	0.2570 mL