2. Apoptosis Metabolic Enzyme/Protease NF-κB Immunology/Inflammation
  3. Apoptosis Endogenous Metabolite Reactive Oxygen Species Caspase
  4. Citicoline

Citicoline  (Synonyms: Cytidine diphosphate-choline; CDP-Choline; Cytidine 5'-diphosphocholine)

Cat. No.: HY-B0739 Purity: 99.87%
SDS
COA
Handling Instructions Technical Support

Citicoline is an endogenous intermediate in the synthesis of phosphatidylcholine which is a component of cell membranes. Citicoline inhibits reactive oxygen species (ROS) and apoptosis. Citicoline can be used for neurological disease and hearing loss study.

For research use only. We do not sell to patients.

Citicoline Chemical Structure

Citicoline Chemical Structure

CAS No. : 987-78-0

Size Price Stock Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in Water
ready for reconstitution
 In-stock
Solution
10 mM * 1 mL in Water In-stock
Solid
100 mg In-stock
500 mg In-stock
1 g   Get quote  
5 g   Get quote  

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 3 publication(s) in Google Scholar

Other Forms of Citicoline:

Citicoline Related Antibodies

Top Publications Citing Use of Products

View All Caspase Isoform Specific Products:

View All Isoforms
Caspase 1 Caspase 2 Caspase 3 Caspase 4 Caspase 5 Caspase 6 Caspase 7 Caspase 8 Caspase 9 Caspase 10 Caspase 12 Caspase Caspase 11 Caspase-13

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

Citicoline is an endogenous intermediate in the synthesis of phosphatidylcholine which is a component of cell membranes. Citicoline inhibits reactive oxygen species (ROS) and apoptosis. Citicoline can be used for neurological disease and hearing loss study[1][2][3].

IC50 & Target

Microbial Metabolite

 

Human Endogenous Metabolite

 

Caspase-8

 

Caspase-9

 

Caspase 3

 

In Vitro

Citicoline (1-100 μM, 24 h or 24 h before glutamate treatment) increases rats retinal cells viability after glutamate-induced excitotoxicity, and has synergistic effects with Tramiprosate (Homotaurine) (HY-14602)[1].
Citicoline (100 μM, 24 h before glutamate treatment and 30 min before HG treatment) decreases proapoptotic effects associated with neuroretinal degeneration such as glutamate-induced excitotoxicity and high glucose (HG)-induced neurotoxicity, and has synergistic effects with Tramiprosate in rats retinal cells[1].
Citicoline (100 μM, 36-48 h) reduces apoptosis in mice cochlear hair cells (HCs) and HEI-OC-1 cells after Neomycin (HY-150520) exposure, decreases the mRNA level of pro-apoptotic factor and increases anti-apoptotic factor, suppresses proapoptotic protein expression[2][3].
Citicoline (100 μM, 36-48 h) attenuates oxidative stress in mice cochlear HCs and HEI-OC-1 cells after Neomycin injury[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Citicoline Related Antibodies

Cell Viability Assay[1]

Cell Line: Rat retinal cells
Concentration: 1, 10, 100 μM
Incubation Time: 24 h or 24 h before glutamate treatment (100 μM)
Result: Did not affect viability of primary retinal cultures.
Significantly increased cell viability after glutamate-induced excitotoxicity, and had synergistic effects with homotaurine

Apoptosis Analysis[1]

Cell Line: Rat retinal cells
Concentration: 100 μM
Incubation Time: 24 h before glutamate treatment and 30 min before HG treatment
Result: Reduced apoptosis induced by administration of glutamate and HG, and had synergistic effects with homotaurine.

Apoptosis Analysis[3]

Cell Line: HEI-OC-1, mice cochlear tissue
Concentration: 10 μM
Incubation Time: 36 or 48 h (pretreatment for 12 h and then treated with 10 mM neomycin together for 12 and 24 h in cochlear tissue and HEI-OC-1, respectively, then allowed to recover in culture medium for another 12
Result: Showed significantly lower numbers of cleaved caspase 3-positive cells and TUNEL-positive cells than the neomycin-only group.
Significantly reduced the percentage of apoptosis.
The protein levels of cleaved caspase 3 were decreased compared with the Neomycin-only group.
Decreased the pro-apoptotic factor Bax, Casp3, Casp8, and Casp9, and increased anti-apoptotic factor Bcl-2 by qPT-PCR analysis.
In Vivo

Citicoline (500-1000 mg/kg; i.p.; 1 h before test) produces an anticonvulsant effect in pentylenetetrazole induced convulsion in male C57Bl/6 mice[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Male C57Bl/6 mice (23-27 g) (The solution of convulsant pentylenetetrazole (1%) was infused intravenously at a flow rate of 0.01 ml/sec.)[2]
Dosage: 500, 1000 mg/kg
Administration: Intraperitoneal injection (i.p.); 1 h before test
Result: Increased the threshold of clonic seizures and tonic phase of seizures with lethal outcome in a dose of 500 mg/kg.
The anticonvulsant effect was most pronounced in a dose of 1000 mg/kg.
Clinical Trial
Molecular Weight

488.32

Formula

C14H26N4O11P2
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

O[C@@H]([C@H]([C@H](N1C(N=C(C=C1)N)=O)O2)O)[C@H]2COP(OCC[N+](C)(C)C)(OP([O-])(O)=O)=O
Structure Classification
Initial Source

Widespread
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 2 years
-20°C 1 year
Solvent & Solubility
In Vitro: 

H2O : 50 mg/mL (102.39 mM; Need ultrasonic)

DMSO : < 1 mg/mL (insoluble or slightly soluble)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.0478 mL 10.2392 mL 20.4784 mL
5 mM 0.4096 mL 2.0478 mL 4.0957 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  PBS

    Solubility: 50 mg/mL (102.39 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
Purity & Documentation

Purity: 99.87%

SDS (393 KB)

COA (258 KB) LCMS (265 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
H2O 1 mM 2.0478 mL 10.2392 mL 20.4784 mL 51.1959 mL
5 mM 0.4096 mL 2.0478 mL 4.0957 mL 10.2392 mL
10 mM 0.2048 mL 1.0239 mL 2.0478 mL 5.1196 mL
15 mM 0.1365 mL 0.6826 mL 1.3652 mL 3.4131 mL
20 mM 0.1024 mL 0.5120 mL 1.0239 mL 2.5598 mL
25 mM 0.0819 mL 0.4096 mL 0.8191 mL 2.0478 mL
30 mM 0.0683 mL 0.3413 mL 0.6826 mL 1.7065 mL
40 mM 0.0512 mL 0.2560 mL 0.5120 mL 1.2799 mL
50 mM 0.0410 mL 0.2048 mL 0.4096 mL 1.0239 mL
60 mM 0.0341 mL 0.1707 mL 0.3413 mL 0.8533 mL
80 mM 0.0256 mL 0.1280 mL 0.2560 mL 0.6399 mL
100 mM 0.0205 mL 0.1024 mL 0.2048 mL 0.5120 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • No file chosen (Maximum size is: 1024 Kb)
  • If you have published this work, please enter the PubMed ID.
  • Your name will appear on the site.

Citicoline Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Citicoline987-78-0Cytidine diphosphate-choline CDP-Choline Cytidine 5'-diphosphocholineApoptosisEndogenous MetaboliteReactive Oxygen SpeciesCaspasereactive oxygen speciesneurologicalhearing lossInhibitorinhibitorinhibit

Your Recently Viewed Products:

Inquiry Online

Your information is safe with us. * Required Fields.

Product Name

  

Requested Quantity *

Applicant Name *

 

Salutation

Email Address *

 

Phone Number *

Department

 

Organization Name *

City

State

Country or Region *

      

Remarks

Bulk Inquiry

Inquiry Information

Product Name:
Citicoline
Cat. No.:
HY-B0739
Quantity:
MCE Japan Authorized Agent:

Customer Review

Thank You for Your Feedback!

Request for HNMR Report

Please fill out this form to request the QC report. We will send it to your Email address shortly.

Your information is safe with us. * Required Fields.

Product Name

  

Lot #

Applicant Name *

 

Email Address *

Phone Number

 

Department *

Organization Name *

 

Country or Region *

Remarks

SAFETY DATA SHEET (SDS)

English - EN (393 KB) Français - FR (393 KB) Deutsch - DE (393 KB) Norwegian - NO (393 KB) Español - ES (393 KB) Swedish - SV (393 KB)

Request for HNMR Report

We have received your request and will respond to you as soon as possible.