2. Induced Disease Models Products Cell Cycle/DNA Damage Cytoskeleton Immunology/Inflammation Autophagy Apoptosis
  3. Nervous System Disease Models Microtubule/Tubulin NOD-like Receptor (NLR) Autophagy Apoptosis
  4. Alzheimer's Disease Models
  5. Colchicine

Colchicine, an orally active alkaloid, is a potent tubulin inhibitor and a microtubule disrupting agent. Colchicine inhibits microtubule polymerization with an IC50 of 3 nM. Colchicine is also a competitive antagonist of the α3 glycine receptors (GlyRs). Colchicine prevents non-steroidal anti-inflammatory drug (NSAID)-induced small intestinal injury by inhibiting activation of the NLRP3 inflammasome. Colchicine has extensive anti-inflammatory, immunosuppressive and strong anti-fibrosis effects and has the potential for gouty arthritis research.

For research use only. We do not sell to patients.

Colchicine Chemical Structure

Colchicine Chemical Structure

CAS No. : 64-86-8

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Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 66 In-stock
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10 mM * 1 mL in DMSO USD 66 In-stock
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Customer Review

Based on 35 publication(s) in Google Scholar

Other Forms of Colchicine:

Colchicine Related Antibodies

Top Publications Citing Use of Products

32 Publications Citing Use of MCE Colchicine

WB
IF

    Colchicine purchased from MedChemExpress. Usage Cited in: Oxid Med Cell Longev. 2020 Jul 15;2020:9173530.  [Abstract]

    Colchicine plays an antipyroptosis role by inhibiting intracellular oxidative stress. HUVECs are treated for 10 or 24 h with different doses of Colchicine (0-10 nM) in the presence of cholesterol crystal (0.5 mg/mL). Intracellular ROS level is detected using a DCFH-DA probe. JC-1 Staining Kit is used for the detection of mitochondrial membrane potential.

    Colchicine purchased from MedChemExpress. Usage Cited in: Oxid Med Cell Longev. 2020 Jul 15;2020:9173530.  [Abstract]

    Colchicine alleviates NLRP3 inflammasome activation relevant in pyroptosis. HUVECs are treated for 24 h with different doses of Colchicine (0-10 nM) in the presence of cholesterol crystal (0.5 mg/mL. Western blotting Is dedicated to examine the protein expression levels of NLRP3, ASC-1, procaspase-1, caspase-1, IL-18, and IL-1β. Colchicine treatment significantly blocks NLRP3 signal activity. Colchicine significantly inhibits CC-induced mRNA and protein expression of IL-18 and IL-1β, particularl

    Colchicine purchased from MedChemExpress. Usage Cited in: Biochim Biophys Acta. 2018 May;1862(5):1134-1147.  [Abstract]

    CS1 and Colchicine (Col) induce the phosphorylation of H1 (T18) and H3 (S29) as detected by western blotting.

    View All NOD-like Receptor (NLR) Isoform Specific Products:

    View All Isoforms
    NLRP3 NLRP1 NOD1 NOD2

    • Biological Activity

    • Purity & Documentation

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    Description

    Colchicine, an orally active alkaloid, is a potent tubulin inhibitor and a microtubule disrupting agent. Colchicine inhibits microtubule polymerization with an IC50 of 3 nM. Colchicine is also a competitive antagonist of the α3 glycine receptors (GlyRs). Colchicine prevents non-steroidal anti-inflammatory drug (NSAID)-induced small intestinal injury by inhibiting activation of the NLRP3 inflammasome. Colchicine has extensive anti-inflammatory, immunosuppressive and strong anti-fibrosis effects and has the potential for gouty arthritis research[1][2][3][4][5].

    IC50 & Target

    Microtubule/Tubulin[1]
    In Vitro

    Colchicine (5 μM; pretreated 24 h) inhibits MIRI-induced apoptosis of H9C2 by regulating the PI3K/AKT/eNOS pathway[3].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Colchicine Related Antibodies

    Apoptosis Analysis[3]

    Cell Line: H9C2 cells
    Concentration: 5 μM
    Incubation Time: Pretreated 24 h
    Result: Caused a significant reduction (4.490%) of early apoptotic cells as compared with the H/R only groups (6.182%).

    Western Blot Analysis[3]

    Cell Line: H9C2 cells
    Concentration: 5 μM
    Incubation Time: Pretreated 24 h
    Result: Observably increased the decreased phosphorylation levels of PI3K, AKT, and eNOS after H/R injury in H9C2 cell.
    In Vivo

    Colchicine can be used to induce Alzheimer's disease models[6].

    Induction of Alzheimer’s disease (AD)[6]
    Background
    Colchicine is a tubulin inhibitor that binds irreversibly to tubulin dimers and inhibits microtubule assembly with disruption of their polymerization, leading to neurofibrillary degeneration and synaptic loss. This causes neurotrophic factor intracellular trafficking to be disrupted, as well as axonal excitotoxicity and oxidative damage.
    Specific Mmodeling Methods
    Rat: Wistar • male • adult, weighing 180-220 g (acclimate: 1 weeks)
    Administration: 7.5 μg in 5 μL/site • intracerebroventricular (icv) injection • single dose
    Note
    (1) For induction of AD, rats are anesthetized using intraperitoneal (i.p.) Thiopental sodium (45 mg/kg) and then positioned in stereotaxic apparatus.
    (2) The stereotaxic coordinates are 0.8 mm posterior to bregma, 1.8 mm lateral to sagittal suture and 3.6 mm beneath the cortical surface.
    (3) Using a 28-gauge micro-syringe (10 μL) 15 μg Colchicine dissolved in 10 μL ACSF is injected bilaterally into the lateral ventricle (7.5 μg in 5 μL/site). The injection rate is 2 μL/min, then the micro-syringe is remained in place for 2 min to avoid withdrawal of the injected fluid.
    (4) Artificial cerebrospinal fluid (ACSF constituents in milli mole: NaCl 147, MgCl2 1.6, KCl 2.9, CaCl2 1.7, and Dextrose 2.2) is freshly prepared.
    Modeling Indicators
    Molecular changes: Induced indicators: Aβ 1-40 and Aβ 1-42 peptides level, MDA concentration, TNF-α level in both hippocampus and prefrontal cortex
    Decrease indicators: SOD activity in both hippocampus and prefrontal cortex
    Correlated Product(s): β-Amyloid (1-42), human TFA (HY-P1363)
    Opposite Product(s): Sacubitril/Valsartan (HY-18204A)

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: Specific-pathogen-free 8-week-old male mice (Wild-type C57BL/6 mice)[4]
    Dosage: 1 or 3 mg/kg
    Administration: Orally; 30 min prior to Indomethacin administration
    Result: Caused less mucosal inflammation and ulceration and a decrease in the size and numbers of lesions compared with vehicle-treated mice with Indomethacin (10 mg/kg; gavage).
    Clinical Trial
    Molecular Weight

    399.44

    Formula

    C22H25NO6
    CAS No.
    Appearance

    Solid

    Color

    White to light yellow

    SMILES

    CC(N[C@H](C1=C2)CCC3=CC(OC)=C(OC)C(OC)=C3C1=CC=C(OC)C2=O)=O
    Structure Classification
    Initial Source
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage
    Powder -20°C 3 years
    4°C 2 years
    In solvent -80°C 2 years
    -20°C 1 year
    Solvent & Solubility
    In Vitro: 

    DMSO : ≥ 48 mg/mL (120.17 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    H2O : ≥ 33.33 mg/mL (83.44 mM)

    *"≥" means soluble, but saturation unknown.

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 2.5035 mL 12.5175 mL 25.0350 mL
    5 mM 0.5007 mL 2.5035 mL 5.0070 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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    In Vivo:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  50% PEG400    50% Saline

      Solubility: 25 mg/mL (62.59 mM); Clear solution; Need ultrasonic

    For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  PBS

      Solubility: 2.78 mg/mL (6.96 mM); Clear solution; Need ultrasonic and warming and heat to 60°C

    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

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    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Calculation results:
    Working solution concentration: mg/mL
    This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
    Purity & Documentation

    Purity: 99.95%

    COA (262 KB) HNMR (276 KB) RP-HPLC (243 KB) LCMS (93 KB)

    Handling Instructions (2659 KB)
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    H2O / DMSO 1 mM 2.5035 mL 12.5175 mL 25.0350 mL 62.5876 mL
    5 mM 0.5007 mL 2.5035 mL 5.0070 mL 12.5175 mL
    10 mM 0.2504 mL 1.2518 mL 2.5035 mL 6.2588 mL
    15 mM 0.1669 mL 0.8345 mL 1.6690 mL 4.1725 mL
    20 mM 0.1252 mL 0.6259 mL 1.2518 mL 3.1294 mL
    25 mM 0.1001 mL 0.5007 mL 1.0014 mL 2.5035 mL
    30 mM 0.0835 mL 0.4173 mL 0.8345 mL 2.0863 mL
    40 mM 0.0626 mL 0.3129 mL 0.6259 mL 1.5647 mL
    50 mM 0.0501 mL 0.2504 mL 0.5007 mL 1.2518 mL
    60 mM 0.0417 mL 0.2086 mL 0.4173 mL 1.0431 mL
    80 mM 0.0313 mL 0.1565 mL 0.3129 mL 0.7823 mL
    DMSO 100 mM 0.0250 mL 0.1252 mL 0.2504 mL 0.6259 mL

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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    Colchicine Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Colchicine64-86-8Microtubule/TubulinNOD-like Receptor (NLR)AutophagyApoptosisOrallyalkaloidtubulinmicrotubuleα3 glycine receptorsGlyRsnon-steroidal anti-inflammatory drugNSAIDintestinal injuryimmunosuppressiveanti-fibrosisgouty arthritisAlzheimer’s diseaseADInhibitorinhibitorinhibit

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