Eicosapentaenoic Acid (Synonyms: EPA; Timnodonic acid)

Name: Eicosapentaenoic Acid
Brand: MedChemExpress
SKU: HY-B0660
Rating: 5.0 (14 reviews)

Cat. No.: HY-B0660 Purity: 98.70%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

Eicosapentaenoic Acid (EPA) is an orally active Omega-3 long-chain polyunsaturated fatty acid (ω-3 LC-PUFA). Eicosapentaenoic Acid exhibits a DNA demethylating action that promotes the re-expression of the tumor suppressor gene CCAAT/enhancer-binding protein δ (C/EBPδ). Eicosapentaenoic Acid activates RAS/ERK/C/EBPβ pathway through H-Ras intron 1 CpG island demethylation in U937 leukemia cells. Eicosapentaenoic Acid can promote relaxation of vascular smooth muscle cells and vasodilation.

For research use only. We do not sell to patients.

Eicosapentaenoic Acid Chemical Structure

CAS No. : 10417-94-4

Size	Price	Stock	Quantity
Liquid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 73	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 73	In-stock	Estimated Time of Arrival: December 31
Liquid
50 mg	USD 66	In-stock	Estimated Time of Arrival: December 31
100 mg	USD 84	In-stock	Estimated Time of Arrival: December 31
500 mg	USD 336	In-stock	Estimated Time of Arrival: December 31
1 g		Get quote
5 g		Get quote

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Based on 14 publication(s) in Google Scholar

Other Forms of Eicosapentaenoic Acid:

Eicosapentaenoic acid ethyl ester In-stock
Eicosapentaenoic Acid-d₅ Get quote
Eicosapentaenoic acid (metformin) Get quote
Eicosapentaenoic Acid sodium Get quote
Eicosapentaenoic Acid (Standard) Get quote

14 Publications Citing Use of MCE Eicosapentaenoic Acid

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Biological Activity
Purity & Documentation
References
Customer Review

Description

IC₅₀ & Target

Human Endogenous Metabolite

Cellular Effect

Cell Line	Type	Value	Description	References
MDA-MB-468	IC₅₀	> 100 μM Compound: EPA	Antiproliferative activity against human MDA-MB-468 cells after 48 hrs by MTT assay Antiproliferative activity against human MDA-MB-468 cells after 48 hrs by MTT assay	[PMID: 22822908]
MDA-MB-468	EC₅₀	> 100 μM Compound: EPA	Proapoptotic activity in human MDA-MB-468 cells assessed as activation of caspase-3 by luminometry Proapoptotic activity in human MDA-MB-468 cells assessed as activation of caspase-3 by luminometry	[PMID: 22822908]
RBL-2H3	IC₅₀	> 50 μM Compound: 6; EPA	Antiallergic activity in rat RBL2H3 cells assessed as inhibition of DNP-HSA-mediated degranulation by measuring decrease in beta-hexosaminidase activity preincubated for 30 mins followed by DNP-HSA stimulation and measured after 30 mins by 4-nitrophenyl 2 Antiallergic activity in rat RBL2H3 cells assessed as inhibition of DNP-HSA-mediated degranulation by measuring decrease in beta-hexosaminidase activity preincubated for 30 mins followed by DNP-HSA stimulation and measured after 30 mins by 4-nitrophenyl 2	[PMID: 31618024]

In Vitro

C/EBPβ phosphorylated form is evident in Eicosapentaenoic Acid (EPA; 100 μM; 24 h) treated cells, whereas it was barely detectable in control, and OA or LA treated U937^[1].
Eicosapentaenoic Acid (100 μM; 1, 3, 24 h) causes a significant increase of H-Ras and N-Ras mRNA levels conditioning for 1 and 3 hours. K-Ras mRNA levels were not affected by Eicosapentaenoic Acid^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Clinical Trial

Molecular Weight

302.45

Formula

C₂₀H₃₀O₂

CAS No.

10417-94-4

Appearance

Liquid (Density: 0.943 g/cm³)

Color

Colorless to light yellow

SMILES

CC/C=C\C/C=C\C/C=C\C/C=C\C/C=C\CCCC(O)=O

Structure Classification

Ketones, Aldehydes, Acids

Initial Source

Microorganisms

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Pure form	-20°C	3 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

Ethanol : ≥ 100 mg/mL (330.63 mM)

DMSO : ≥ 30 mg/mL (99.19 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	3.3063 mL	16.5317 mL	33.0633 mL
5 mM	0.6613 mL	3.3063 mL	6.6127 mL
10 mM	0.3306 mL	1.6532 mL	3.3063 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% EtOH 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (8.27 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL EtOH stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.
Protocol 2

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: 2.08 mg/mL (6.88 mM); Suspended solution; Need ultrasonic

This protocol yields a suspended solution of 2.08 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 3

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: 2.08 mg/mL (6.88 mM); Suspended solution; Need ultrasonic

This protocol yields a suspended solution of 2.08 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 4

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.08 mg/mL (6.88 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 50% PEG300 50% Saline
Solubility: 10 mg/mL (33.06 mM); Suspended solution; Need ultrasonic
Protocol 2

Add each solvent one by one: 15% Cremophor EL 85% Saline
Solubility: 10 mg/mL (33.06 mM); Suspended solution; Need ultrasonic

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 98.70%

Select Batch:

Data Sheet (268 KB) SDS (476 KB)

COA (237 KB) HNMR (240 KB) LCMS (87 KB)

Handling Instructions (2659 KB)

References

[1]. Martins, J.G., EPA but not DHA appears to be responsible for the efficacy of omega-3 long chain polyunsaturated fatty acid supplementation in depression: evidence from a meta-analysis of randomized controlled trials. J Am Coll Nutr, 2009. 28(5): p. 525-42. [Content Brief]

[2]. Miaozhen Pan, et al. Dietary ω-3 polyunsaturated fatty acids are protective for myopia. Proc Natl Acad Sci U S A. 2021 Oct 26;118(43):e2104689118. [Content Brief]

[3]. Shun-he Wang, et al. Endogenous omega-3 long-chain fatty acid biosynthesis from alpha-linolenic acid is affected by substrate levels, gene expression, and product inhibition. RSC Adv., 2017, 7, 40946-40951.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO / Ethanol	1 mM	3.3063 mL	16.5317 mL	33.0633 mL	82.6583 mL
	5 mM	0.6613 mL	3.3063 mL	6.6127 mL	16.5317 mL
	10 mM	0.3306 mL	1.6532 mL	3.3063 mL	8.2658 mL
	15 mM	0.2204 mL	1.1021 mL	2.2042 mL	5.5106 mL
	20 mM	0.1653 mL	0.8266 mL	1.6532 mL	4.1329 mL
	25 mM	0.1323 mL	0.6613 mL	1.3225 mL	3.3063 mL
	30 mM	0.1102 mL	0.5511 mL	1.1021 mL	2.7553 mL
	40 mM	0.0827 mL	0.4133 mL	0.8266 mL	2.0665 mL
	50 mM	0.0661 mL	0.3306 mL	0.6613 mL	1.6532 mL
	60 mM	0.0551 mL	0.2755 mL	0.5511 mL	1.3776 mL
	80 mM	0.0413 mL	0.2066 mL	0.4133 mL	1.0332 mL
Ethanol	100 mM	0.0331 mL	0.1653 mL	0.3306 mL	0.8266 mL

Eicosapentaenoic Acid Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Eicosapentaenoic Acid10417-94-4EPA Timnodonic acidEndogenous MetaboliteHistone Demethylaselong-chain polyunsaturated fatty acidω-3 LC-PUFADNA demethylatingCCAATC/EBPδRASERKC/EBPβH-Ras intron 1 CpG islandInhibitorinhibitorinhibit

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Eicosapentaenoic Acid (Synonyms: EPA; Timnodonic acid)

Customer Review

Other Forms of Eicosapentaenoic Acid:

Eicosapentaenoic Acid Related Antibodies

14 Publications Citing Use of MCE Eicosapentaenoic Acid

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

View All Histone Demethylase Isoform Specific Products:

Biological Activity

Purity & Documentation

References

Customer Review

Eicosapentaenoic Acid Related Antibodies

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

Eicosapentaenoic Acid Related Classifications

Keywords:

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