Histamine (Synonyms: Ergamine)

Name: Histamine
Brand: MedChemExpress
SKU: HY-B1204
Rating: 5.0 (9 reviews)

Cat. No.: HY-B1204 Purity: 99.99%: FAQs
Data Sheet SDS COA Handling Instructions

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Histamine is an organic nitrogen compound that participates in local immune responses, regulates intestinal physiological functions, and acts as a neurotransmitter.

For research use only. We do not sell to patients.

Histamine Chemical Structure

CAS No. : 51-45-6

Size	Price	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in Water ready for reconstitution	USD 66	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in Water	USD 66	In-stock	Estimated Time of Arrival: December 31
Solid
100 mg	USD 60	In-stock	Estimated Time of Arrival: December 31
200 mg		Get quote
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Based on 9 publication(s) in Google Scholar

Other Forms of Histamine:

Histamine phosphate In-stock
Histamine-α,α,β,β-d₄ dihydrochloride Get quote
Histamine dihydrochloride Get quote
Histamine (Standard) Get quote

9 Publications Citing Use of MCE Histamine

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View All Isoforms

H₁ Receptor H₂ Receptor H₃ Receptor H₄ Receptor Histamine Receptor

Biological Activity
Purity & Documentation
References
Customer Review

Description

Histamine is an organic nitrogen compound that participates in local immune responses, regulates intestinal physiological functions, and acts as a neurotransmitter.

IC₅₀ & Target

Human Endogenous Metabolite

Cellular Effect

Cell Line	Type	Value	Description	References
CHO	EC₅₀	640 nM Compound: Histamine	Agonist activity at human recombinant histamine H3 receptor expressed in CHO cells assessed as [35S]GTPgammaS binding after 1 hr by liquid scintillation counting Agonist activity at human recombinant histamine H3 receptor expressed in CHO cells assessed as [35S]GTPgammaS binding after 1 hr by liquid scintillation counting	[PMID: 20384344]
HEK293	EC₅₀	13 nM Compound: Histamine	Agonist activity at human SF-His6-tagged histamine H4 receptor expressed in HEK293 cells assessed as inhibition of forskolin-stimulated response incubated for 5 hrs by luciferase reporter gene assay Agonist activity at human SF-His6-tagged histamine H4 receptor expressed in HEK293 cells assessed as inhibition of forskolin-stimulated response incubated for 5 hrs by luciferase reporter gene assay	[PMID: 27007611]
HEK293	EC₅₀	94 nM Compound: Histamine	Agonist activity at mouse SF-His6-tagged histamine H4 receptor expressed in HEK293 cells assessed as inhibition of forskolin-stimulated response incubated for 5 hrs by luciferase reporter gene assay Agonist activity at mouse SF-His6-tagged histamine H4 receptor expressed in HEK293 cells assessed as inhibition of forskolin-stimulated response incubated for 5 hrs by luciferase reporter gene assay	[PMID: 27007611]
Sf9	EC₅₀	11 nM Compound: Histamine	Agonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assay Agonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assay	[PMID: 27007611]
Sf9	EC₅₀	11.6 nM Compound: 1	Activity at human recombinant histamine H4 receptor-RGS4 fusion protein expressed in Sf9 cells coexpressing Galphai2 and G-beta-1-gamma-2 by steady-state GTPase activity assay Activity at human recombinant histamine H4 receptor-RGS4 fusion protein expressed in Sf9 cells coexpressing Galphai2 and G-beta-1-gamma-2 by steady-state GTPase activity assay	[PMID: 18950149]
Sf9	EC₅₀	1200 nM Compound: 1	Activity at human recombinant histamine H2 receptor-Gsalpha fusion protein expressed in Sf9 cells by steady-state GTPase activity assay Activity at human recombinant histamine H2 receptor-Gsalpha fusion protein expressed in Sf9 cells by steady-state GTPase activity assay	[PMID: 18950149]
Sf9	EC₅₀	1200 nM Compound: 1	Activity at guinea pig recombinant histamine H2 receptor-Gsalpha fusion protein expressed in Sf9 cells by steady-state GTPase activity assay Activity at guinea pig recombinant histamine H2 receptor-Gsalpha fusion protein expressed in Sf9 cells by steady-state GTPase activity assay	[PMID: 18950149]
Sf9	EC₅₀	13 nM Compound: Histamine	Agonist activity at human histamine H3 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assay Agonist activity at human histamine H3 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assay	[PMID: 27007611]
Sf9	EC₅₀	190 nM Compound: Histamine	Agonist activity at human histamine H1 receptor expressed in sf9 cell membrane co-expressing RGS4 or RGS9 assessed as increase in 32Pi level incubated for 20 mins by liquid scintillation counting in presence of [gamma32P]GTP Agonist activity at human histamine H1 receptor expressed in sf9 cell membrane co-expressing RGS4 or RGS9 assessed as increase in 32Pi level incubated for 20 mins by liquid scintillation counting in presence of [gamma32P]GTP	[PMID: 27007611]
Sf9	EC₅₀	190 nM Compound: 1	Activity at human recombinant histamine H1 receptor expressed in Sf9 cells coexpressing RGS4 by steady-state GTPase activity assay Activity at human recombinant histamine H1 receptor expressed in Sf9 cells coexpressing RGS4 by steady-state GTPase activity assay	[PMID: 18950149]
Sf9	EC₅₀	25.1 nM Compound: 1	Activity at human recombinant histamine H3 receptor expressed in Sf9 cells coexpressing Galphai, G-beta-1-gamma-2 and RGS4 by steady-state GTPase activity assay Activity at human recombinant histamine H3 receptor expressed in Sf9 cells coexpressing Galphai, G-beta-1-gamma-2 and RGS4 by steady-state GTPase activity assay	[PMID: 18950149]

In Vivo

Histamine can be used in animal modeling to create gastrointestinal ulcer models. After intravenous injection, the maximum concentration and AUC of histamine in liver and liver tumor tissues are higher than those in subcutaneous tissues^[1].

Induction of Gastrointestinal Ulcer^[2]

Background

Histamine can cause increased gastric acid secretion, decreased mucus production, reflux of pancreatic juice, poor gastric blood flow, and thus gastric ulcers. Stress can cause increased gastrointestinal motility, making the gastric folds more susceptible to damage when exposed to acid.

Specific Mmodeling Methods

Guinea Pig: male • albino • Administration: 5 mg/kg • i.p. • single dose

Note

(1) No eating or drinking is allowed 24 hours before the experiment.
(2) The animals are sacrificed 2 hours after histamine injection, and treated with 1% formalin to check for gastric ulcers.

Modeling Indicators

The ulcers were punctate or elongated. After the model was dissected, the ulcer index (the sum of the lengths of the lesions) was measured under a microscope to be 3.4 mm.

Correlated Product(s): Serotonin (HY-B1473A); Reserpine (HY-N0480)

Opposite Product(s): Cimetidine (HY-14289)

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Clinical Trial

Molecular Weight

111.15

Formula

C₅H₉N₃

CAS No.

51-45-6

Appearance

Solid

Color

White to yellow

SMILES

NCCC1=CN=CN1

Structure Classification

Others

Initial Source

Microorganisms

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

-20°C, stored under nitrogen, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen, away from moisture)

Solvent & Solubility

In Vitro:

H₂O : 125 mg/mL (1124.63 mM; Need ultrasonic)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	8.9970 mL	44.9851 mL	89.9701 mL
5 mM	1.7994 mL	8.9970 mL	17.9940 mL
10 mM	0.8997 mL	4.4985 mL	8.9970 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: PBS
Solubility: 100 mg/mL (899.70 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Purity: 99.99%

Select Batch:

Data Sheet (276 KB) SDS (645 KB)

COA (245 KB) HNMR (227 KB) RP-HPLC (241 KB) MS (176 KB)

Handling Instructions (2659 KB)

References

[1]. Rizell M, Naredi P, Lindnér P, Hellstrand K, Sarno M, Jansson PA. Histamine pharmacokinetics in tumor and host tissues after bolus-dose administration in the rat. Life Sci. 2002 Jan 11;70(8):969-76. [Content Brief]

[2]. Okabe S, et al. Effects of cimetidine, a histamine H2-receptor antagonist, on various experimental gastric and duodenal ulcers. Am J Dig Dis. 1977 Aug;22(8):677-84. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O	1 mM	8.9970 mL	44.9851 mL	89.9701 mL	224.9253 mL
	5 mM	1.7994 mL	8.9970 mL	17.9940 mL	44.9851 mL
	10 mM	0.8997 mL	4.4985 mL	8.9970 mL	22.4925 mL
	15 mM	0.5998 mL	2.9990 mL	5.9980 mL	14.9950 mL
	20 mM	0.4499 mL	2.2493 mL	4.4985 mL	11.2463 mL
	25 mM	0.3599 mL	1.7994 mL	3.5988 mL	8.9970 mL
	30 mM	0.2999 mL	1.4995 mL	2.9990 mL	7.4975 mL
	40 mM	0.2249 mL	1.1246 mL	2.2493 mL	5.6231 mL
	50 mM	0.1799 mL	0.8997 mL	1.7994 mL	4.4985 mL
	60 mM	0.1500 mL	0.7498 mL	1.4995 mL	3.7488 mL
	80 mM	0.1125 mL	0.5623 mL	1.1246 mL	2.8116 mL
	100 mM	0.0900 mL	0.4499 mL	0.8997 mL	2.2493 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Histamine Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Histamine51-45-6ErgamineHistamine ReceptorEndogenous MetaboliteInhibitorinhibitorinhibit

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Histamine (Synonyms: Ergamine)

Customer Review

Other Forms of Histamine:

Histamine Related Antibodies

9 Publications Citing Use of MCE Histamine

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

View All Histamine Receptor Isoform Specific Products:

Biological Activity

Purity & Documentation

References

Customer Review

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

Histamine Related Classifications

Keywords:

Your Recently Viewed Products:

Customer Review

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