Loganin (Synonyms: Loganoside)

Name: Loganin
Brand: MedChemExpress
SKU: HY-N0512
Rating: 5.0 (4 reviews)

Cat. No.: HY-N0512 Purity: 99.82%: FAQs
Data Sheet SDS COA Handling Instructions Technical Support

Loganin is a type of iridoid glycoside compound that possesses anti-inflammatory, antioxidant, and antitumor properties, and offers protective effects against acute lung injury and pulmonary fibrosis. Loganin exerts its protective effects against LPS (HY-D1056)-mediated inflammation and oxidative stress by upregulating the Nrf2/HO-1 signaling pathway, and it reduces neuroinflammation caused by spinal cord injury (SCI).

For research use only. We do not sell to patients.

Loganin Chemical Structure

CAS No. : 18524-94-2

Size	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	In-stock	Estimated Time of Arrival: December 31
Solid
5 mg	In-stock	Estimated Time of Arrival: December 31
10 mg	In-stock	Estimated Time of Arrival: December 31
50 mg	In-stock	Estimated Time of Arrival: December 31
100 mg	In-stock	Estimated Time of Arrival: December 31
200 mg	Get quote
500 mg	Get quote

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Customer Review

Based on 4 publication(s) in Google Scholar

Other Forms of Loganin:

Loganin (Standard) Get quote

4 Publications Citing Use of MCE Loganin

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TNFRSF1A/CD120a TNFRSF5/CD40 TNFRSF6/Fas/CD95 TNFRSF7/CD27 TNFRSF8/CD30 TNFRSF9/4-1BB/CD137 TNFRSF16/NGF Receptor/CD271 TNFRSF18/GITR/CD357

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IL-1 IL-2 IL-3 IL-4 IL-5 IL-6 IL-8 IL-10 IL-12 IL-13 IL-15 IL-17 IL-23 IL-7 IL-33 IL-22

Biological Activity
Purity & Documentation
References
Customer Review

Description

Cellular Effect

Cell Line	Type	Value	Description	References
Caco-2	IC₅₀	1.28 μg/mL Compound: 1	Cytotoxic activity against Homo sapiens (human) Caco2 cells incubated for 4 hr followed by compound wash-out measured after doubling time by MTT assay Cytotoxic activity against Homo sapiens (human) Caco2 cells incubated for 4 hr followed by compound wash-out measured after doubling time by MTT assay	10.1007/s00044-011-9832-9
Caco-2	IC₅₀	3.45 μg/mL Compound: 1	Cytotoxic activity against Homo sapiens (human) Caco2 cells by clonogenic assay Cytotoxic activity against Homo sapiens (human) Caco2 cells by clonogenic assay	10.1007/s00044-011-9832-9
COLO 320	IC₅₀	1 μg/mL Compound: 1	Cytotoxic activity against Homo sapiens (human) COLO320 cells incubated for 4 hr followed by compound wash-out measured after doubling time by MTT assay Cytotoxic activity against Homo sapiens (human) COLO320 cells incubated for 4 hr followed by compound wash-out measured after doubling time by MTT assay	10.1007/s00044-011-9832-9
COLO 320	IC₅₀	2.2 μg/mL Compound: 1	Cytotoxic activity against Homo sapiens (human) COLO320 cells by clonogenic assay Cytotoxic activity against Homo sapiens (human) COLO320 cells by clonogenic assay	10.1007/s00044-011-9832-9
Huh-7	IC₅₀	16 μg/mL Compound: 5, ASB-00012351-010	Antiviral activity against HCV infected in human Huh7 cells assessed as inhibition of viral replication after 72 hrs by NS5A staining Antiviral activity against HCV infected in human Huh7 cells assessed as inhibition of viral replication after 72 hrs by NS5A staining	[PMID: 19904996]
MCF7	IC₅₀	4.85 μg/mL Compound: 1	Cytotoxic activity against Homo sapiens (human) MCF7 cells incubated for 4 hr followed by compound wash-out measured after doubling time by MTT assay Cytotoxic activity against Homo sapiens (human) MCF7 cells incubated for 4 hr followed by compound wash-out measured after doubling time by MTT assay	10.1007/s00044-011-9832-9
MCF7	IC₅₀	6.45 μg/mL Compound: 1	Cytotoxic activity against Homo sapiens (human) MCF7 cells by clonogenic assay Cytotoxic activity against Homo sapiens (human) MCF7 cells by clonogenic assay	10.1007/s00044-011-9832-9
PA-1	IC₅₀	1.45 μg/mL Compound: 1	Cytotoxic activity against Homo sapiens (human) PA1 cells incubated for 4 hr followed by compound wash-out measured after doubling time by MTT assay Cytotoxic activity against Homo sapiens (human) PA1 cells incubated for 4 hr followed by compound wash-out measured after doubling time by MTT assay	10.1007/s00044-011-9832-9
PA-1	IC₅₀	4.85 μg/mL Compound: 1	Cytotoxic activity against Homo sapiens (human) PA1 cells by clonogenic assay Cytotoxic activity against Homo sapiens (human) PA1 cells by clonogenic assay	10.1007/s00044-011-9832-9
WRL68	IC₅₀	1.86 μg/mL Compound: 1	Cytotoxic activity against Homo sapiens (human) WRL68 cells incubated for 4 hr followed by compound wash-out measured after doubling time by MTT assay Cytotoxic activity against Homo sapiens (human) WRL68 cells incubated for 4 hr followed by compound wash-out measured after doubling time by MTT assay	10.1007/s00044-011-9832-9
WRL68	IC₅₀	3.2 μg/mL Compound: 1	Cytotoxic activity against Homo sapiens (human) WRL68 cells by clonogenic assay Cytotoxic activity against Homo sapiens (human) WRL68 cells by clonogenic assay	10.1007/s00044-011-9832-9

In Vitro

Loganin (0-50 µM; 24 h) shows significant cytotoxicity to RAW264.7 at high concentrations^[3].
Loganin (0-30 µM; 1 h) significantly reduces the production of NO, PGE2, TNF-α, and IL-1β in RAW264.7 macrophages stimulated by LPS (100 ng/ml, 24 h) in a dose-dependent manner, and increases the expression of Nrf2 and HO-1 in a concentration-dependent manner^[3].
Loganin (30 µM; 1 h) alleviates LPS-mediated ROS generation in RAW264.7 macrophages^[3].
Loganin (0-150 μg/ml; 48 h) reduces the survival rate, migration and invasion ability of Huh7 cells^[6].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[3]

Cell Line:	RAW264.7 cells
Concentration:	0,5,10, 30 and 50 µM
Incubation Time:	24 h
Result:	Did not affect cytotoxicity at concentrations below 30 µM, but significant cytotoxicity was observed at 50 µM.

Western Blot Analysis^[3]

Cell Line:	RAW264.7 cells
Concentration:	0,5,10 and 30 µM
Incubation Time:	1 h
Result:	Significantly inhibited LPS-induced upregulation of iNOS and COX-2 . Compared with cells treated with loganin or LPS alone, Nrf2 protein expression was significantly upregulated in the nucleus of loganin-treated cells, but HO-1 expression was only observed in the cytoplasm.

Western Blot Analysis^[6]

Cell Line:	Huh7 cells
Concentration:	0、50、100 and 150 μg/ml
Incubation Time:	48 h
Result:	Increased E-cadherin levels and decreased N-cadherin and vimentin levels in cells.

In Vivo

Loganin (1-10 mg/kg; i.p.; once daily from day 1 to day 28 after SCI) dose-dependently reduced the degree of neurological damage in rats with SCI model^[4].
Loganin (80-200 mg/kg; i.g.; 8 weeks of Bleomycin induction, twice a week from week 4 (day 28)) concentration-dependently reduced BLM-induced lung injury in mice^[5].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Spinal cord injury rat model^[4]
Dosage:	1, 5 and 10 mg/kg
Administration:	i.p.; Administer once daily from 1 to 28 days after SCI
Result:	Improved the results of the inclined board test at a dose of 5 mg/kg on the 28th day after surgery and significantly improved the results of the inclined board test at a dose of 10 mg/kg from the 14th day after surgery.

Animal Model:	Bleomycin-induced pulmonary fibrosis model in mice^[5]
Dosage:	80、160 和 200 mg/kg
Administration:	i.g.; Bleomycin induction for 8 weeks, twice a week from the fourth week (day 28)
Result:	Improved alveolar structural destruction and interstitial inflammatory infiltration and reduced the hydroxyproline content in lung tissue.

Molecular Weight

390.38

Formula

C₁₇H₂₆O₁₀

CAS No.

18524-94-2

Appearance

Solid

Color

White to off-white

SMILES

O[C@H]([C@@H](O)[C@@H]1O)[C@](O[C@@H]1CO)([H])O[C@H]2[C@@]([C@H]3C)([H])[C@@](C[C@@H]3O)([H])C(C(OC)=O)=CO2

Structure Classification

Initial Source

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

Solvent & Solubility

In Vitro:

DMSO : 50 mg/mL (128.08 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.5616 mL	12.8080 mL	25.6161 mL
5 mM	0.5123 mL	2.5616 mL	5.1232 mL
10 mM	0.2562 mL	1.2808 mL	2.5616 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (6.40 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (6.40 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (6.40 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.82%

Select Batch:

Data Sheet (287 KB) SDS (393 KB)

COA (258 KB) HNMR (273 KB) LCMS (253 KB)

Handling Instructions (2659 KB)

References

[1]. Kim MJ, et al. Loganin protects against pancreatitis by inhibiting NF-κB activation. Eur J Pharmacol. 2015 Oct 15;765:541-50. [Content Brief]

[2]. Tsai WH, et al. Ba-Wei-Di-Huang-Wan through its active ingredient loganin counteracts substance P-enhanced NF-κB/ICAM-1 signaling in rats with bladder hyperactivity. Neurourol Urodyn. 2015 Aug 19. [Content Brief]

[3]. Park C, et al. Loganin Inhibits Lipopolysaccharide-Induced Inflammation and Oxidative Response through the Activation of the Nrf2/HO-1 Signaling Pathway in RAW264.7 Macrophages. Biol Pharm Bull. [Content Brief]

[4]. Huang X, et al. Loganin reduces neuroinflammation and promotes functional recovery after spinal cord injury through inhibiting NF-κB/NLRP3 signalling. Toxicon. 2023 Aug 15;232:107202. [Content Brief]

[5]. Chen L, et al. Protective effect and mechanism of loganin and morroniside on acute lung injury and pulmonary fibrosis. Phytomedicine. 2022 May;99:154030. [Content Brief]

[6]. CHEN, et al. Loganin inhibits Liver Cancer Cell Proliferation and Metastasis via Blocking Mitogen-Activated Protein Kinase Signaling Pathway. Indian Journal of Pharmaceutical Sciences 86.1 (2024).

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.5616 mL	12.8080 mL	25.6161 mL	64.0402 mL
	5 mM	0.5123 mL	2.5616 mL	5.1232 mL	12.8080 mL
	10 mM	0.2562 mL	1.2808 mL	2.5616 mL	6.4040 mL
	15 mM	0.1708 mL	0.8539 mL	1.7077 mL	4.2693 mL
	20 mM	0.1281 mL	0.6404 mL	1.2808 mL	3.2020 mL
	25 mM	0.1025 mL	0.5123 mL	1.0246 mL	2.5616 mL
	30 mM	0.0854 mL	0.4269 mL	0.8539 mL	2.1347 mL
	40 mM	0.0640 mL	0.3202 mL	0.6404 mL	1.6010 mL
	50 mM	0.0512 mL	0.2562 mL	0.5123 mL	1.2808 mL
	60 mM	0.0427 mL	0.2135 mL	0.4269 mL	1.0673 mL
	80 mM	0.0320 mL	0.1601 mL	0.3202 mL	0.8005 mL
	100 mM	0.0256 mL	0.1281 mL	0.2562 mL	0.6404 mL

Loganin Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Loganin18524-94-2LoganosideReactive Oxygen SpeciesTNF ReceptorInterleukin RelatedTumor Necrosis Factor ReceptorTNFRILInhibitorinhibitorinhibit

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Loganin (Synonyms: Loganoside)

Customer Review

Other Forms of Loganin:

Loganin Related Antibodies

4 Publications Citing Use of MCE Loganin

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•Related Screening Libraries:

•Related Small Molecules:

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View All TNF Receptor Isoform Specific Products:

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Biological Activity

Purity & Documentation

References

Customer Review

Loganin Related Antibodies

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

Loganin Related Classifications

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