Niacin (Synonyms: Nicotinic acid; Vitamin B3)

Name: Niacin
Brand: MedChemExpress
SKU: HY-B0143
Rating: 5.0 (9 reviews)

Cat. No.: HY-B0143 Purity: 99.93%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

Niacin (Vitamin B3) is an orally active water-soluble B3 vitamin that is an essential nutrient for humans. Niacin (Vitamin B3) plays a key role in energy metabolism, cell signaling cascades regulating gene expression and apoptosis. Niacin (Vitamin B3) is also used in the study of cardiovascular diseases.

For research use only. We do not sell to patients.

Niacin Chemical Structure

CAS No. : 59-67-6

Size	Price	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 61	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 61	In-stock	Estimated Time of Arrival: December 31
Solid
500 mg	USD 55	In-stock	Estimated Time of Arrival: December 31
1 g	USD 66	In-stock	Estimated Time of Arrival: December 31
5 g	USD 86	In-stock	Estimated Time of Arrival: December 31
10 g		Get quote
50 g		Get quote

or Bulk Inquiry

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 9 publication(s) in Google Scholar

Other Forms of Niacin:

Niacin-13C3,15N Get quote
Niacin-d₄ Get quote
Niacin-¹³C₆ Get quote
Niacin-¹⁵N,¹³C₃ Get quote
Niacin hydrochloride Get quote

8 Publications Citing Use of MCE Niacin

: Niacin purchased from MedChemExpress. Usage Cited in: Glia. 2018 Feb;66(2):256-278. [Abstract]; Representative images and quantitative analysis showing the effects of Niacin (1 mM, 10 min) and MK-6892 (10 lM, 10 min) on Akt phosphorylation levels in primary cultured microglia.

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

Biological Activity
Purity & Documentation
References
Customer Review

Description

IC₅₀ & Target

Microbial Metabolite

Human Endogenous Metabolite

Cellular Effect

Cell Line	Type	Value	Description	References
A-431	EC₅₀	0.73 μM Compound: Niacin	Activation of GPR109A in human A431 cells assessed as suppression of forskolin-induced cAMP production after 30 mins Activation of GPR109A in human A431 cells assessed as suppression of forskolin-induced cAMP production after 30 mins	[PMID: 26784936]
Adipocyte	IC₅₀	0.07 μM Compound: Niacin	Inhibition of lipolysis in IBMX-stimulated human adipocytes assessed as glycerol release after 5 hrs Inhibition of lipolysis in IBMX-stimulated human adipocytes assessed as glycerol release after 5 hrs	[PMID: 19309152]
Adipocyte	EC₅₀	0.9 μM Compound: NA	Inhibition of isoproterenol-induced lipolysis in human subcutaneous adipocytes after 5 hrs Inhibition of isoproterenol-induced lipolysis in human subcutaneous adipocytes after 5 hrs	[PMID: 17452318]
CCRF-CEM	IC₅₀	> 500 μM Compound: Niacin	Cytotoxicity against human CEM cells assessed as growth inhibition after 72 hrs by coulter counter method Cytotoxicity against human CEM cells assessed as growth inhibition after 72 hrs by coulter counter method	[PMID: 28238612]
CHO	EC₅₀	0.027 μM Compound: Niacin	Agonist activity at GPR109a expressed in CHO cells assessed as inhibition of forskolin-stimulated cAMP production by HTRF assay Agonist activity at GPR109a expressed in CHO cells assessed as inhibition of forskolin-stimulated cAMP production by HTRF assay	[PMID: 20363624]
CHO	EC₅₀	1 μM Compound: nicotinic acid	Agonist activity at human GPR109A receptor expressed in CHO cells by [35S]GTPgammaS binding assay Agonist activity at human GPR109A receptor expressed in CHO cells by [35S]GTPgammaS binding assay	[PMID: 18752940]
CHO	IC₅₀	1 μM Compound: niacin	Agonist activity at human niacin receptor expressed in CHO cells by GTPgammaS binding assay Agonist activity at human niacin receptor expressed in CHO cells by GTPgammaS binding assay	[PMID: 18477506]
CHO	EC₅₀	51 nM Compound: niacin, Niaspan	Agonist activity at human GPR109a expressed in CHO cells assessed as decrease in forskolin-stimulated cAMP production by HTRF assay Agonist activity at human GPR109a expressed in CHO cells assessed as decrease in forskolin-stimulated cAMP production by HTRF assay	[PMID: 22435740]
CHO-K1	EC₅₀	1 μM Compound: Niacin	Agonist activity at human GPR109A expressed in CHOK1 cells by [35S]GTPgammaS binding assay Agonist activity at human GPR109A expressed in CHOK1 cells by [35S]GTPgammaS binding assay	[PMID: 19309152]
CHO-K1	EC₅₀	1 μM Compound: niacin	Agonist activity at human GPR109A expressed in CHOK1 cells by [35S]GTP-gamma-S binding assay Agonist activity at human GPR109A expressed in CHOK1 cells by [35S]GTP-gamma-S binding assay	[PMID: 17994679]
CHO-K1	EC₅₀	1.4 μM Compound: nicotinic acid	Agonist activity at human GPR109A receptor expressed in CHO-K1 cells by [35S]GTPgammaS guanine nucleotide exchange assay Agonist activity at human GPR109A receptor expressed in CHO-K1 cells by [35S]GTPgammaS guanine nucleotide exchange assay	[PMID: 18760600]
CHO-K1	EC₅₀	47 nM Compound: Nicotinic acid	Agonist activity at HCA2 receptor (unknown origin) expressed in CHOK1 cells assessed as ERK1/2 phosphorylation by ELISA Agonist activity at HCA2 receptor (unknown origin) expressed in CHOK1 cells assessed as ERK1/2 phosphorylation by ELISA	[PMID: 25737085]
CHO-K1	EC₅₀	527 nM Compound: NA	Agonist activity at cloned human GPR109A receptor expressed in CHO-K1 cells by [35S]GTPgammaS binding assay Agonist activity at cloned human GPR109A receptor expressed in CHO-K1 cells by [35S]GTPgammaS binding assay	[PMID: 20184326]
CHO-K1	EC₅₀	580 nM Compound: NA	Activation of GPR109A receptor in CHOK1 cells assessed as ERK1/2 MAP kinase activation by ELISA Activation of GPR109A receptor in CHOK1 cells assessed as ERK1/2 MAP kinase activation by ELISA	[PMID: 17452318]
CHO-K1	IC₅₀	67.3 μM Compound: NA	Agonist activity at GPR109A receptor expressed in CHOK1 cells assessed as inhibition of forskolin-induced intracellular cAMP production by Flashplate assay Agonist activity at GPR109A receptor expressed in CHOK1 cells assessed as inhibition of forskolin-induced intracellular cAMP production by Flashplate assay	[PMID: 17452318]
COS-7	EC₅₀	0.78 μM Compound: 3	Agonist activity at human recombinant GPR109a transfected in african green monkey COS-7 cells after 20 mins by [35S]GTPgammaS binding assay Agonist activity at human recombinant GPR109a transfected in african green monkey COS-7 cells after 20 mins by [35S]GTPgammaS binding assay	[PMID: 24900524]
HEK293	IC₅₀	> 500 μM Compound: niacin	Inhibition of human MATE1-mediated ASP+ uptake expressed in HEK293 cells after 1.5 mins by fluorescence assay Inhibition of human MATE1-mediated ASP+ uptake expressed in HEK293 cells after 1.5 mins by fluorescence assay	[PMID: 23241029]
HEK-293T	IC₅₀	249 nM Compound: Nicotinic acid	Displacement of [3H]-nicotinic acid from N-flag-tagged human HCA2 receptor expressed in HEK293T cell membranes by scintillation counting analysis Displacement of [3H]-nicotinic acid from N-flag-tagged human HCA2 receptor expressed in HEK293T cell membranes by scintillation counting analysis	[PMID: 25737085]
HEK-293T	EC₅₀	5 μM Compound: Niacin	Agonist activity at GPR109A receptor (unknown origin) expressed in HEK293T cell membranes after 30 mins by [35S]GTPgammaS binding based microbeta scintillation counting analysis Agonist activity at GPR109A receptor (unknown origin) expressed in HEK293T cell membranes after 30 mins by [35S]GTPgammaS binding based microbeta scintillation counting analysis	[PMID: 32199732]
HL-60	CC₅₀	> 200 μM Compound: Niacin	Cytotoxicity against human HL60 cells after 48 hrs by MTT assay Cytotoxicity against human HL60 cells after 48 hrs by MTT assay	[PMID: 28238612]
HSC-2	CC₅₀	> 200 μM Compound: Niacin	Cytotoxicity against human HSC2 cells after 48 hrs by MTT assay Cytotoxicity against human HSC2 cells after 48 hrs by MTT assay	[PMID: 28238612]
HSC-3	CC₅₀	> 200 μM Compound: Niacin	Cytotoxicity against human HSC3 cells after 48 hrs by MTT assay Cytotoxicity against human HSC3 cells after 48 hrs by MTT assay	[PMID: 28238612]
HSC-4	CC₅₀	> 200 μM Compound: Niacin	Cytotoxicity against human HSC4 cells after 48 hrs by MTT assay Cytotoxicity against human HSC4 cells after 48 hrs by MTT assay	[PMID: 28238612]
L1210	IC₅₀	> 500 μM Compound: Niacin	Cytotoxicity against mouse L1210 cells assessed as growth inhibition after 48 hrs by coulter counter method Cytotoxicity against mouse L1210 cells assessed as growth inhibition after 48 hrs by coulter counter method	[PMID: 28238612]

In Vitro

Niacin (0-900 μM, 42 hours) significantly increases GSH levels and decreases ROS levels, and affects the expression of genes related to apoptosis and lipid metabolism^[1].
Niacin (0-40 μM, 24 hours) can inhibit cancer invasive activity at low dose but with no influence on proliferation^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

RT-PCR^[1]

Cell Line:	Cumulus cells and oocytes of prepubertal gilts
Concentration:	600 μM
Incubation Time:	42 hours
Result:	Up-regulated the relative expression of the anti-apoptotic gene BCL2 and lipid metabolism gene ACACA while down-regulated the pro-apoptotic gene BAX.

Cell Proliferation Assay^[2]

Cell Line:	Rat ascites hepatoma cell line of AH109A
Concentration:	0-40 μM
Incubation Time:	24 hours
Result:	Had no effect on the proliferation of AH109A cells but suppressed cell invasion from 2.5 μM to 40 μM.

In Vivo

Niacin (subcutaneous injection, 3-300 mg/kg, once) can induce vasodilation in a dose-dependent manner within minutes in male C57BL/6 mice^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Male C57BL/6 mice^[3]
Dosage:	3-300 mg/kg
Administration:	Subcutaneous injection; once
Result:	Induced vasodilation in a dose-dependent manner.

Clinical Trial

Molecular Weight

123.11

Formula

C₆H₅NO₂

CAS No.

59-67-6

Appearance

Solid

Color

White to off-white

SMILES

O=C(C1=CC=CN=C1)O

Structure Classification

Ketones, Aldehydes, Acids

Initial Source

Microorganisms

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Store at room temperature 3 years

In solvent	-80°C	1 year
	-20°C	6 months

Solvent & Solubility

In Vitro:

DMSO : 50 mg/mL (406.14 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : 10 mg/mL (81.23 mM; Need ultrasonic)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	8.1228 mL	40.6138 mL	81.2275 mL
5 mM	1.6246 mL	8.1228 mL	16.2455 mL
10 mM	0.8123 mL	4.0614 mL	8.1228 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: 2.08 mg/mL (16.90 mM); Suspended solution; Need ultrasonic

This protocol yields a suspended solution of 2.08 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.08 mg/mL (16.90 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.08 mg/mL (16.90 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: PBS
Solubility: 12.5 mg/mL (101.53 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.96%

Select Batch:

Data Sheet (279 KB) SDS (393 KB)

COA (248 KB) HNMR (123 KB) RP-HPLC (225 KB) LCMS (98 KB)

Handling Instructions (2659 KB)

References

[1]. Areeg M Almubarak, et al. Supplementation with Niacin during in vitro maturation improves the quality of porcine embryos. Theriogenology. 2021 Jul 15;169:36-46. doi: 10.1016/j.theriogenology.2021.04.005. Epub 2021 Apr 18. [Content Brief]

[2]. Nobuhiro Hirakawa, et al. Anti-invasive activity of niacin and trigonelline against cancer cells. Biosci Biotechnol Biochem. 2005 Mar;69(3):653-8. [Content Brief]

[3]. Kang Cheng, et al. Antagonism of the prostaglandin D2 receptor 1 suppresses nicotinic acid-induced vasodilation in mice and humans. Proc Natl Acad Sci U S A. 2006 Apr 25;103(17):6682-7. [Content Brief]

[4]. Bruckert E, et al. Meta-analysis of the effect of nicotinic acid alone or in combination on cardiovascular events and atherosclerosis. Atherosclerosis. 2010 Jun;210(2):353-61. [Content Brief]

[5]. Wan P, et al. Pellagra: a review with emphasis on photosensitivity. Br J Dermatol. 2011 Jun;164(6):1188-200. [Content Brief]

[6]. Ishii N, et al. Pellagra among chronic alcoholics: clinical and pathological study of 20 necropsy cases. J Neurol Neurosurg Psychiatry. 1981 Mar;44(3):209-15. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O / DMSO	1 mM	8.1228 mL	40.6138 mL	81.2275 mL	203.0688 mL
	5 mM	1.6246 mL	8.1228 mL	16.2455 mL	40.6138 mL
	10 mM	0.8123 mL	4.0614 mL	8.1228 mL	20.3069 mL
	15 mM	0.5415 mL	2.7076 mL	5.4152 mL	13.5379 mL
	20 mM	0.4061 mL	2.0307 mL	4.0614 mL	10.1534 mL
	25 mM	0.3249 mL	1.6246 mL	3.2491 mL	8.1228 mL
	30 mM	0.2708 mL	1.3538 mL	2.7076 mL	6.7690 mL
	40 mM	0.2031 mL	1.0153 mL	2.0307 mL	5.0767 mL
	50 mM	0.1625 mL	0.8123 mL	1.6246 mL	4.0614 mL
	60 mM	0.1354 mL	0.6769 mL	1.3538 mL	3.3845 mL
	80 mM	0.1015 mL	0.5077 mL	1.0153 mL	2.5384 mL
DMSO	100 mM	0.0812 mL	0.4061 mL	0.8123 mL	2.0307 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Niacin Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Niacin59-67-6Nicotinic acid Vitamin B3Vitamin B3Vitamin B 3Vitamin B-3AutophagyEndogenous Metaboliteapoptosiscardiovascular diseasescancerInhibitorinhibitorinhibit

Please select a local distributor ナミキ商事株式会社コスモ・バイオ株式会社
Product Name			Salutation
Applicant Name *			Email Address *
Phone Number *			Organization Name *
Department *			Requested quantity *
Country or Region *
Remarks

Product Name			Lot #
Applicant Name *			Email Address *
Phone Number			Department *
Organization Name *			Country or Region *
Remarks

Name
Email *

	Sorry, but the email address you supplied was invalid.

Niacin (Synonyms: Nicotinic acid; Vitamin B3)

Customer Review

Other Forms of Niacin:

Niacin Related Antibodies

8 Publications Citing Use of MCE Niacin

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

Biological Activity

Purity & Documentation

References

Customer Review

Niacin Related Antibodies

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

Niacin Related Classifications

Keywords:

Your Recently Viewed Products:

Customer Review

Request for HNMR Report

SAFETY DATA SHEET (SDS)

Request for HNMR Report