Ononin (Synonyms: Ononoside; Formononetin 7-O-β-D-glucopyranoside)

Name: Ononin
Brand: MedChemExpress
SKU: HY-N0270
Rating: 5.0 (5 reviews)

Cat. No.: HY-N0270 Purity: 99.96%: FAQs
Data Sheet SDS COA Handling Instructions

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Ononin is an isoflavone that inhibits the growth of Pluchea lanceolata in soil.

For research use only. We do not sell to patients.

Ononin Chemical Structure

CAS No. : 486-62-4

Size	Price	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 58	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 58	In-stock	Estimated Time of Arrival: December 31
Solid
5 mg	USD 53	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 86	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 346	In-stock	Estimated Time of Arrival: December 31
100 mg	USD 528	In-stock	Estimated Time of Arrival: December 31
200 mg		Get quote
500 mg		Get quote

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This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 5 publication(s) in Google Scholar

Other Forms of Ononin:

Ononin (Standard) Get quote

5 Publications Citing Use of MCE Ononin

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Biological Activity
Purity & Documentation
Customer Review

Description

Ononin is an isoflavone that inhibits the growth of Pluchea lanceolata in soil.

Cellular Effect

Cell Line	Type	Value	Description	References
HepG2	IC₅₀	40.8 μM Compound: 9	Anticancer activity against human HepG2 cells assessed as cell viability after 48 hrs by MTT assay Anticancer activity against human HepG2 cells assessed as cell viability after 48 hrs by MTT assay	[PMID: 24974349]

Molecular Weight

430.40

Formula

C₂₂H₂₂O₉

CAS No.

486-62-4

Appearance

Solid

Color

White to off-white

SMILES

O[C@H]([C@@H](O)[C@H](O)[C@@H](CO)O1)[C@@H]1OC2=CC=C(C(C(C3=CC=C(OC)C=C3)=CO4)=O)C4=C2

Structure Classification

Initial Source

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (232.34 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.3234 mL	11.6171 mL	23.2342 mL
5 mM	0.4647 mL	2.3234 mL	4.6468 mL
10 mM	0.2323 mL	1.1617 mL	2.3234 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

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Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (4.83 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.08 mg/mL (4.83 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.08 mg/mL (4.83 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.96%

Select Batch:

Data Sheet (246 KB) SDS (393 KB)

COA (248 KB) HNMR (307 KB) LCMS (120 KB)

Handling Instructions (2659 KB)

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.3234 mL	11.6171 mL	23.2342 mL	58.0855 mL
	5 mM	0.4647 mL	2.3234 mL	4.6468 mL	11.6171 mL
	10 mM	0.2323 mL	1.1617 mL	2.3234 mL	5.8086 mL
	15 mM	0.1549 mL	0.7745 mL	1.5489 mL	3.8724 mL
	20 mM	0.1162 mL	0.5809 mL	1.1617 mL	2.9043 mL
	25 mM	0.0929 mL	0.4647 mL	0.9294 mL	2.3234 mL
	30 mM	0.0774 mL	0.3872 mL	0.7745 mL	1.9362 mL
	40 mM	0.0581 mL	0.2904 mL	0.5809 mL	1.4521 mL
	50 mM	0.0465 mL	0.2323 mL	0.4647 mL	1.1617 mL
	60 mM	0.0387 mL	0.1936 mL	0.3872 mL	0.9681 mL
	80 mM	0.0290 mL	0.1452 mL	0.2904 mL	0.7261 mL
	100 mM	0.0232 mL	0.1162 mL	0.2323 mL	0.5809 mL

Ononin Related Classifications

Others

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Ononin486-62-4Ononoside Formononetin 7-O-β-D-glucopyranosideOthersInhibitorinhibitorinhibit

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Ononin (Synonyms: Ononoside; Formononetin 7-O-β-D-glucopyranoside)

Customer Review

Other Forms of Ononin:

Ononin Related Antibodies

5 Publications Citing Use of MCE Ononin

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

Biological Activity

Purity & Documentation

Customer Review

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

Ononin Related Classifications

Keywords:

Your Recently Viewed Products:

Customer Review

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SAFETY DATA SHEET (SDS)

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