2. Immunology/Inflammation Metabolic Enzyme/Protease Stem Cell/Wnt JAK/STAT Signaling
  3. PD-1/PD-L1 HIF/HIF Prolyl-Hydroxylase STAT
  4. Panaxadiol

Panaxadiol  (Synonyms: 20(R)-Panaxadiol)

Cat. No.: HY-N0596 Purity: ≥98.0%
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Panaxadiol (20(R)-Panaxadiol) is an orally active HIF-1α/STAT3 inhibitor. Panaxadiol can suppress HIF-1α and STAT3 then lead to downregulation of programmed cell death-ligand 1 (PD-L1) expression. Panaxadiol shows anticancer, cardioprotective, anti-arrhythmic, and antioxidative activities.

For research use only. We do not sell to patients.

Panaxadiol Chemical Structure

Panaxadiol Chemical Structure

CAS No. : 19666-76-3

Size Price Stock Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 97 In-stock
Solution
10 mM * 1 mL in DMSO USD 97 In-stock
Solid
1 mg USD 45 In-stock
5 mg USD 96 In-stock
10 mg USD 120 In-stock
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Based on 1 publication(s) in Google Scholar

Other Forms of Panaxadiol:

Panaxadiol Related Antibodies

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Description

Panaxadiol (20(R)-Panaxadiol) is an orally active HIF-1α/STAT3 inhibitor. Panaxadiol can suppress HIF-1α and STAT3 then lead to downregulation of programmed cell death-ligand 1 (PD-L1) expression. Panaxadiol shows anticancer, cardioprotective, anti-arrhythmic, and antioxidative activities[1][2].

In Vitro

Panaxadiol (1-10 μM, 12 h) inhibits PD-L1 expression in human colon cancer cells[1].
Panaxadiol (1-10 μM, 12 h) inhibits the expression of PD-L1 by suppressing HIF-1α and STAT3 in HCT116 cells[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Panaxadiol Related Antibodies

Western Blot Analysis[1]

Cell Line: HCT116, SW620 and HT-29 colon cancer cells
Concentration: 1, 3, and 10 μM
Incubation Time: 12 hours
Result: Decreased the expression of PD-L1 protein and mRNA in a dose-dependent manner.

Western Blot Analysis[1]

Cell Line: HCT116 cells
Concentration: 1, 3, and 10 μM
Incubation Time: 12 hours
Result: Inhibited hypoxia-induced nuclear accumulation of HIF-1α in a dose-dependent manner.
Inhibited STAT3 phosphorylation at Tyr705 in a dose-dependent manner under both normoxic and hypoxic conditions.
In Vivo

Panaxadiol (oral gavage; 10 or 30 mg/kg; once every 3 days; 30 d) inhibits the growth of HCT116 cells in a xenograft model[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: BALB/c athymic nude mice injected with HCT116 cells[1]
Dosage: 10 or 30 mg/kg
Administration: Oral gavage; 10 or 30 mg/kg; once every 3 days; 30 days
Result: Inhibited the protein levels of HIF-1α, p-STAT-3 (Tyr705), PD-L1 and VEGF in tumour tissues in a dose-dependent manner.
Molecular Weight

460.73

Formula

C30H52O3
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

C[C@@]1(CC[C@@H]2O)[C@](C[C@H]3O)([H])[C@](CC[C@@]1([H])C2(C)C)(C)[C@@]4(C)[C@@]3([H])[C@]([C@](CCC5)(C)OC5(C)C)([H])CC4
Structure Classification
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 2 years
-20°C 1 year
Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (217.05 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Ethanol : 20 mg/mL (43.41 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.1705 mL 10.8523 mL 21.7047 mL
5 mM 0.4341 mL 2.1705 mL 4.3409 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
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Concentration
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Volume
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Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.5 mg/mL (5.43 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% Corn Oil

    Solubility: ≥ 2.5 mg/mL (5.43 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation

Purity: ≥98.0%

COA (255 KB) HNMR (271 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
Ethanol / DMSO 1 mM 2.1705 mL 10.8523 mL 21.7047 mL 54.2617 mL
5 mM 0.4341 mL 2.1705 mL 4.3409 mL 10.8523 mL
10 mM 0.2170 mL 1.0852 mL 2.1705 mL 5.4262 mL
15 mM 0.1447 mL 0.7235 mL 1.4470 mL 3.6174 mL
20 mM 0.1085 mL 0.5426 mL 1.0852 mL 2.7131 mL
25 mM 0.0868 mL 0.4341 mL 0.8682 mL 2.1705 mL
30 mM 0.0723 mL 0.3617 mL 0.7235 mL 1.8087 mL
40 mM 0.0543 mL 0.2713 mL 0.5426 mL 1.3565 mL
DMSO 50 mM 0.0434 mL 0.2170 mL 0.4341 mL 1.0852 mL
60 mM 0.0362 mL 0.1809 mL 0.3617 mL 0.9044 mL
80 mM 0.0271 mL 0.1357 mL 0.2713 mL 0.6783 mL
100 mM 0.0217 mL 0.1085 mL 0.2170 mL 0.5426 mL
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Panaxadiol Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Panaxadiol19666-76-320(R)-PanaxadiolPD-1/PD-L1HIF/HIF Prolyl-HydroxylaseSTATPD-1/Programmed death-ligand 1Hypoxia-inducible factorsHIFsHIF-PHanticancer activitycardioprotective activityanti-arrhythmic activityantioxidative activityHCT116SW620HT-29Inhibitorinhibitorinhibit

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